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Response surface optimization of lipid and protein extractions from Spirulina platensis using ultrasound assisted osmotic shock method

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Abstract

In this study, we optimized the process for extracting lipids and proteins from wet biomasses of Spirulina sp. using a 4-kHz ultrasonic osmotic shock method with ultrasound enhancement at a constant frequency of 40 kHz. Optimization was conducted using a response surface methodology (RSM) at an osmotic NaCl concentration of 10–30%, solvent:biomass ratio of 5–15 v/w, and extraction times of 20–50 min. The present osmotic shock method with ultrasound irradiation increased lipid yields to 6.65% in the presence of 11.9% NaCl, a solvent:biomass ratio of 12:1 v/w, and a 22-min extraction time, and protein yields to 43.96% with 15.12% NaCl, a solvent:biomass ratio of 10:1 v/w, and a 30-min extraction time.

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Acknowledgement

The authors would like to thank Ministry of Research, Technology and Higher Education for their grants through Hibah Kompetensi of Year 2018. All staffs of Center of Biomass and Renewable Energy (CBIORE) are highly appreciated.

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Authors and Affiliations

  1. Department of Chemical Engineering, Faculty of Engineering, Diponegoro University, Jl. Prof Soedarto, SH Tembalang, Semarang, 50275, Indonesia

    H. Hadiyanto & Nais Pinta Adetya

  2. Master Program of Environmental Science, School of Postgraduate Studies, Diponegoro University, Jl. Imam Bardjo No 3-5, Semarang, Indonesia

    H. Hadiyanto

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  1. H. Hadiyanto
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  2. Nais Pinta Adetya
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Correspondence to Nais Pinta Adetya.

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Hadiyanto, H., Adetya, N.P. Response surface optimization of lipid and protein extractions from Spirulina platensis using ultrasound assisted osmotic shock method. Food Sci Biotechnol 27, 1361–1368 (2018). https://doi.org/10.1007/s10068-018-0389-y

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  • DOI: https://doi.org/10.1007/s10068-018-0389-y

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