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Release of N-nitrosamines and N-nitrosatable substances from baby bottle teats and rubber kitchen tools in Korea

  • Se-Jong Park
  • Mi-Jin Jeong
  • So-Ra Park
  • Jae Chun Choi
  • Heeju Choi
  • MeeKyung Kim
Article
  • 45 Downloads

Abstract

Ensuring the safety of baby bottle teats and kitchen tools made from rubber is critical. Therefore, the migration of N-nitrosamines and N-nitrosatable substances from 30 teats and 45 kitchen tools to artificial saliva was analyzed by liquid chromatography–tandem mass spectrometry. The method was validated by assessing the limits of detection (0.46–3.87 μg/kg), limits of quantification (1.38–11.73 μg/kg), and recoveries (86.3–108.6%) of seven compounds. Nitrosodimethylamine (NDMA), N-nitrosopiperidine, and N-nitrosomorpholine (NMOR) migrated from baby bottle teats at concentrations of not detected (ND) to 3.67 μg/kg. NDMA and NMOR concentrations ranged from ND to 1.72 μg/kg after migration from 45 rubber kitchen tools. N-nitrosatable substances ranged from ND to 42.16 μg/kg after migration from baby bottle teats but did not migrate from rubber kitchen tools. All tested products were considered safe for use, as N-nitrosamine and N-nitrosatable substance levels did not exceed the permitted management specifications.

Keywords

N-nitrosamines N-nitrosatable substances Baby bottle teat Rubber kitchen tool LC–MS/MS 

Notes

Acknowledgements

This research was supported by a Grant from the Korea Ministry of Food and Drug Safety (14161MFDS015).

Compliance with ethical standards

Conflict of interest

No potential conflicts of interest are reported by the authors.

References

  1. 1.
    Challis BC. Nutrition and nitrosamine formation. J. Proc. Nutr. Soc. 44: 95–100 (1985).CrossRefGoogle Scholar
  2. 2.
    IARC. List of Classifications. International Agency Research on Cancer, Lyon, France (2015). Available from: http://monographs.iarc.fr/ENG/Classification/latest_classif.php.
  3. 3.
    CEN. EN 12868: Child use and care articles–methods for determining the release of N-nitrosamines and N-nitrosatable Substances from elastomer or rubber teats and soothers. European Committee for Standardization (1999).Google Scholar
  4. 4.
    Bouma K, Nab FM, Schothorst RC. Migration of N-nitrosamines, N-nitrosatable substances and 2-mercaptobenzthiazol from baby bottle teats and soothers: a Dutch retail survey. Food Addit. Contam. 20: 853–858 (2003).CrossRefGoogle Scholar
  5. 5.
    EU. Commission Directive 93/11/EEC. Concerning the release of the N-nitrosamines and N-nitrosatable substances from elastomer or rubber teats and soothers. Official Journal of European Union L93/37 (1993). Available from: http://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:31993L0011&from=en.
  6. 6.
    ASTM. Standard specification for volatile N-nitrosamine levels in rubber nipples on pacifiers. F1313-90. American Society for Testing and Materials (2011). Available from: http://filesinstrument.com.cn/bbs/upfile/2007113010288.pdf;  https://doi.org/10.1520/f1313-90r99.
  7. 7.
    European Committee for Standardization. Child use and care articles. Methods for determining the release of N-nitrosamines and N-nitrosatable substances from elastomer or rubber teats and soothers. EN 12868 (1999).Google Scholar
  8. 8.
    Masahiro T, Kazuko M, Hideo Y, Yohya W. Determination of volatile N-nitrosamines in rubber nipples by gas chromatography using thermal energy analyzer. J. Anal. Sci. 2: 577–580 (1986).CrossRefGoogle Scholar
  9. 9.
    Nrishinha PS, Santosh C, Kushwaha Stephen WS, Steven GC. Nitrosamines in baby bottle nipples and pacifiers: occurrence, migration, and effect of infant formulas and fruit juices on in vitro formation of nitrosamines under simulated gastric conditions. J. Agric. Food Chem. 33: 428–433 (1985).CrossRefGoogle Scholar
  10. 10.
    Heavy DC, Fazio T. Estimation of volatile N-nitrosamines in rubber nipples for babies’ bottles. J. Food Chem. Toxicol. 20: 939–944 (1982).CrossRefGoogle Scholar
  11. 11.
    Sung JH, Kwak IS, Park SK, Kim HI, Lim HS, Park HJ, Kim SH. Liquid chromatography–tandem mass spectrometry determination of N-nitrosamines released from rubber or elastomer teats and soothers. Food Addit. Contam. 27: 1745–1754 (2010).CrossRefGoogle Scholar
  12. 12.
    Feng D, Wang H, Cheng X, Wang J, Ning L, Zhou Q, Zhou Y, Yang Q. Detection and toxicity assessment of nitrosamines migration from latex gloves in the Chinese market. Int. J. Hyg. Environ. Health. 212: 533–540 (2009).CrossRefGoogle Scholar
  13. 13.
    Feng D, Yang H, Qi D, Li Z. Extraction, confirmation, and screening of non-target compounds in silicone rubber teats by purge-and-trap and SPME combined with GC-MS. Polym. Test. 56: 91–98 (2016).CrossRefGoogle Scholar
  14. 14.
    Motoh M, Yamaguchi M, Kawamura Y. Analysis of N-nitrosamine migration from rubber teats and soothers. J. Anal. Chem. 4: 277–285 (2013).CrossRefGoogle Scholar
  15. 15.
    Sen NP, Kushwaha SC, Seaman SW, Clarkson SG. Nitrosamines in baby bottle nipples and pacifiers: Occurrence, migration, and effect of infant formulas and fruit juices on in vitro formation of nitrosamines under simulated gastric conditions. J Agric. Food Chem. 33: 428–433 (1985).CrossRefGoogle Scholar
  16. 16.
    Niessner G, Klampfl CW. Direct comparison of solid-phase extraction and solid-phase microextraction for the gas chromatographicdetermination of dibenzylamine in artificial saliva leachates from baby bottle teats. Anal. Chimi. Acta. 414: 133–140 (2000).CrossRefGoogle Scholar
  17. 17.
    Reche F, Garrigos MC, Marin ML, Canto A, Jimenez A. Optimization of parameters for the supercritical fluid extraction in the determination of N-nitrosamines in rubbers. J Chromatog A. 963: 419–426 (2002).CrossRefGoogle Scholar
  18. 18.
    MFDS. Standards and specifications for food utensil, containers and packages. 206-210. Ministry of Food and Drug Safety (2015). Available from: https://www.mfds.go.kr/eng/eng/download.do;jsessionid=mp8xvroGqonS7pLm4YMS2aPFE3a4135esS9AVWaRCcsDaaRjglpV6IphR6ABjjIu?boardCode=17837&boardSeq=70089&fileSeq=1.
  19. 19.
    AOAC. Appendix K, Part I: Guidelines for single laboratory validation of chemical methods for dietary supplements and botanicals. Association of Official Analytical Chemists (2013). Available from: http://www.eoma.aoac.org/app_k.pdf.
  20. 20.
    Bouma K, Nab FM, Schothorst RC. Migration of N-nitrosamines, N-nitrosatable substances and 2-mercaptobenzthiazol from baby bottle teats and soothers: a Dutch retail survey. Food Add. Contam. 20: 853–858 (2003).CrossRefGoogle Scholar
  21. 21.
    Feng D, Liu L, Zhao L, Zhou Q, Tan T. Evaluation of simulant migration of volatile nitrosamines from Latex Gloves and Balloons by HS-SPME-GC-MS. J Chromatog Sci. 50: 733–738 (2012).CrossRefGoogle Scholar

Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Se-Jong Park
    • 1
  • Mi-Jin Jeong
    • 1
  • So-Ra Park
    • 1
  • Jae Chun Choi
    • 1
  • Heeju Choi
    • 1
  • MeeKyung Kim
    • 1
  1. 1.Food Additives and Packaging DivisionNational Institute of Food and Drug Safety EvaluationCheongju-siRepublic of Korea

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