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Optimization of ultrasonic-assisted extraction of phenolic compounds from Justicia spicigera leaves

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Abstract

A Box–Behnken design (Extraction-time, pulse-cycle, sonication-amplitude) was employed to extract phenolic compounds from Justicia spicigera leaves by ultrasonic-assisted extraction. The muicle leaves extracts were analyzed measuring total phenolic compounds and antioxidant capacity. According to response surface methodology the optimal conditions of ultrasonic-assisted extraction to obtain the highest soluble phenolic content were 2 min (extraction time) for 0.7 s (pulse cycle) at 55% of sonication amplitude. Under these optimal conditions, the total phenolic content was higher when was used ultrasonic-assisted extraction (54.02 mg/g) than stirring (46.46 mg/g) and thermal decoction (47.76 mg/g); however, the antioxidant capacity from J. spicigera extracts did not increase by ultrasonic-assisted extraction. The extracts or aqueous infusions from J. spicigera leaves are used for therapeutic proposes, therefore the ultrasonic-assisted extraction is a useful technology to improve the extraction of phytochemicals from J. spicigera leaves.

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Acknowledgements

This research was financially supported by Tecnólogico Nacional de Mexico (Grant No. 5611.15-P).

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Authors and Affiliations

  1. Laboratorio de Microbiología de Alimentos, Universidad de Guadalajara, Centro Universitario de los Altos, Carretera a Yahualica km 7.5, 47630, Tepatitlán de Morelos, Jalisco, Mexico

    Luis Miguel Anaya-Esparza

  2. Laboratorio Integral de Investigación en Alimentos, Instituto Tecnológico de Tepic, Av Instituto Tecnológico No 2595, Col Lagos del Country, 63175, Tepic, Nayarit, Mexico

    Dení Ramos-Aguirre, Víctor Manuel Zamora-Gasga & Efigenia Montalvo-González

  3. Facultad de Ciencias Naturales, Universidad Autonoma de Queretaro, Avenida de las Ciencias S/N, 76230, Juriquilla, Querétaro, Mexico

    Elhadi Yahia

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  1. Luis Miguel Anaya-Esparza
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  2. Dení Ramos-Aguirre
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  3. Víctor Manuel Zamora-Gasga
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Correspondence to Efigenia Montalvo-González.

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Anaya-Esparza, L.M., Ramos-Aguirre, D., Zamora-Gasga, V.M. et al. Optimization of ultrasonic-assisted extraction of phenolic compounds from Justicia spicigera leaves. Food Sci Biotechnol 27, 1093–1102 (2018). https://doi.org/10.1007/s10068-018-0350-0

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  • DOI: https://doi.org/10.1007/s10068-018-0350-0

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