Effects of Chios mastic gum and exercise on physical characteristics, blood lipid markers, insulin resistance, and hepatic function in healthy Japanese men


The effects of Chios mastic gum (Pistacia lentiscus var. Chia) and exercise on physical characteristics, blood lipid markers, insulin resistance, and hepatic function were investigated in healthy, non-smoking Japanese men aged ≥ 40 years. Participants were allocated to control (C, 5 g/day placebo powder, n = 7), mastic (M, 5 g/day mastic powder, n = 7), or mastic plus physical activity groups (M + PA, 5 g/day mastic powder and 30-min exercise three times/week, n = 7), and measurements were taken at baseline, 3 and 6 months. Serum triglycerides were significantly reduced at 3 months in M and M + PA compared with C (P < 0.05). Serum insulin and homeostatic model assessment of insulin resistance values were significantly reduced at 3 and 6 months in M + PA, and at 6 months in M, compared with C (P < 0.05). These results indicate that Chios mastic gum intake for 6 months reduced serum triglyceride and insulin concentrations, and additional exercise enhanced the effect on insulin.

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  1. 1.

    Fukazawa T, Smyrniodis I, Konishi M, Takahashi M, Kim H, Numao S, Sakamoto S. Comparative study between Western and Asian pharmaceutical plants: the efficacy of the Greek mastic and the Japanese ume. Deltos. 43: 31–38 (2014)

    Google Scholar 

  2. 2.

    Lemonakis N, Magiatis P, Kostomitsopoulos N, Skaltsounis AL, Tamvakopoulos C. Oral administration of chios mastic gum or extracts in mice: quantification of triterpenic acids by liquid chromatography-tandem mass spectrometry. Planta Med. 77: 1916–1923 (2011)

    Article  CAS  PubMed  Google Scholar 

  3. 3.

    Zacharopoulos C, Barbikas E. Chios Mastiha Historic and Folkloric references. The Chios Mastiha Growers Association, Chios, pp. 2–37 (2008)

    Google Scholar 

  4. 4.

    Wellmann M. Pedanii ioscuridis Anazarbei de Materia Medica Libri Quinque. Weidmann, Berlin (1907)

    Google Scholar 

  5. 5.

    Huwez FU, Thirwell D, Cockayne A, Ala’Aldeen DA. Mastic gum kills Helicobacter pylori. New Engl. J. Med. 339: 1946 (1998)

    Article  CAS  PubMed  Google Scholar 

  6. 6.

    Dabos K, Sfika E, Vlatta LJ, Giannikopoulos G. The effect of mastic gum on Helicobacter pylori: a randomized pilot study. Phytomedicine. 17: 296–299 (2010)

    Article  CAS  PubMed  Google Scholar 

  7. 7.

    Topitsoglou-Themeli V, Kolokotronis A, Dangalis P, Lambrou D. Chios mastiha and oral hygiene: differentiation in microbial plaque formation. Pedodontia 2: 56–59 (1985)

    Google Scholar 

  8. 8.

    Takahashi K, Fukazawa M, Motohira H, Ochiai K, Nishikawa H, Miyata T. A pilot study on antiplaque effects of mastic chewing gum in oral cavity. J. Periodontol. 74: 501–505 (2003)

    Article  PubMed  Google Scholar 

  9. 9.

    Magiatis P, Melliou E, Skaltsounis AL, Chinou IB, Mitaku S. Chemical composition and antimicrobial activity of the essential oils of pistacia lentiscus var. chia. Planta Med. 65: 749–752 (1999)

    Article  CAS  PubMed  Google Scholar 

  10. 10.

    Triantafyllou A, Bikineyeva A, Dikalova A, Nazarewicz R, Lerakis S, Dikalov S. Anti-inflammatory activity of chios mastiha gum is associated with inhibition of TNF-alpha induced oxidative stress. Nutr. J. 10: 64 (2011)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. 11.

    Abdel-Rahman AHY, Youssef SAM. Mastiha as an antioxidant. J. Am. Oil Chem. Soc. 52: 423 (1975)

    Article  CAS  Google Scholar 

  12. 12.

    Triantafyllou A, Chaviaras N, Sergentanis TN, Protopappa E, Tsaknis JS. Chios mastic gum modulates serum biochemical parameters in a human population. J. Ethnopharmacol. 111: 43–49 (2007)

    Article  PubMed  Google Scholar 

  13. 13.

    Petersen RK, Christensen KB, Assimopoulou AN, Fretté X, Papageorgiou VP, Kristiansen K, Kouskoumvekaki I. Pharmacophore-driven identification of PPARγ agonists from natural sources. J. Comput. Aided Mol. Des. 25: 107–116 (2011)

    Article  CAS  PubMed  Google Scholar 

  14. 14.

    Komiyama Y. Ministerial notification no. 430 of the Ministry of Health, Labour and Welfare: a basic direction for comprehensive implementation of national health promotion. Available from: http://www.mhlw.go.jp/file/06-Seisakujouhou-10900000-enkoukyoku/0000047330.pdf. Accessed Dec 27, 2012.

  15. 15.

    Vallianou I, Peroulis N, Pantazis P, Hadzopoulou-Cladaras M. Camphene, a plant-derived monoterpene, reduces plasma cholesterol and triglycerides in hyperlipidemic rats independently of HMG-CoA reductase activity. PLoS One 6: e20516 (2011)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. 16.

    Georgiadis I, Karatzas T, Korou M, Katsilambros N, Perrea D. Beneficial health effects of chios gum mastic and peroxisome proliferator-activates receptors: indications of common mechanisms. J. Med. Food. 18: 1–10 (2015)

    Article  CAS  PubMed  Google Scholar 

  17. 17.

    Takahashi K, Yoshimura Y, Kaimoto T, Kunii D, Komatsu T, Yamamoto S. Validation of a food frequency questionnaire based on food groups for estimating individual nutrient intake. Jpn. J. Nutr. 59: 221–232 (2001)

    Article  Google Scholar 

  18. 18.

    Georgiadis I, Karatzas T, Korou LM, Agrogiannis G, Vlachos IS, Pantopoulou A, Tzanetakou IP, Katsilambros N, Perrea DN. Evaluation of chios mastic gum on lipid and glucose metabolism in diabetic mice. J. Med. Food. 17: 393–399 (2014)

    Article  CAS  PubMed  Google Scholar 

  19. 19.

    Kartalis A, Didagelos M, Georgiadis I, Benetos G, Smyrnioudis N, Marmaras H, Voutas P, Zotika C, Garoufalis S, Andrikopoulos G. Effect of chios mastic gum on cholesterol and glucose levels of healthy volunteers: a prospective, randomized, placebo-controlled, pilot study (CHIOS-MASTIHA). Eur. J. Prev. Cardiol. 23: 722–729 (2016)

    Article  PubMed  Google Scholar 

  20. 20.

    Berger J, Moller D. The mechanism of action of PPARs. Annu. Rev. Med. 53: 409–435 (2002)

    Article  CAS  PubMed  Google Scholar 

  21. 21.

    Nissen SE, Nicholls SJ, Wolski K, Howey DC, McErlean E, Wang MD, Gomez EV, Russo JM. Effects of a potent and selective PPAR-alpha agonist in patients with atherogenic dyslipidemia or hypercholesterolemia: two randomized controlled trials. J. Am. Med. Assoc. 297: 1362–1373 (2007)

    Article  CAS  Google Scholar 

  22. 22.

    Wagner K, Wagner N. Peroxisome proliferator-activated receptor beta/delta (PPAR-β/δ) acts as a regulator of metabolism linked to multiple cellular functions. Pharmacol. Ther. 125: 423–435 (2010)

    Article  CAS  PubMed  Google Scholar 

  23. 23.

    Wang YX, Lee CH, Tiep S, Yu RT, Ham J, Kang H, Evans RM. Peroxisome-proliferator-activated receptor delta activates fat metabolism to prevent obesity. Cell 113: 159–170 (2003)

    Article  CAS  PubMed  Google Scholar 

  24. 24.

    Auboeuf D, Rieusset J, Fajas L, Vallier P, Frering V, Riou JP, Staels B, Auwerx J, Laville M, Vidal H. Tissue distribution and quantification of the expression of mRNAs of peroxisome proliferator-activated receptors and liver X receptor-alpha in humans: no alterations in adipose tissue of obese and NIDDM patients. Diabetes 46: 1319–1327 (1997)

    Article  CAS  PubMed  Google Scholar 

  25. 25.

    Fajas L, Auboeuf D, Raspé E, Schoonjans K, Lefebvre AM, Saladin R, Najib J, Laville M, Fruchart JC, Deeb S, Vidal-Puig A, Flier J, Briggs MR, Staels B, Vidal H, Auwerx J. The organization, promoter analysis and expression of the human PPAR gamma gene. J. Biol. Chem. 272: 18779–18789 (1997)

    Article  CAS  PubMed  Google Scholar 

  26. 26.

    Kahara T, Takamura T, Hayakawa T, Nagai Y, Yamaguchi H, Katsuki T, Katsuki K, Katsuki M, Kobayashi K. PPARγ gene polymorphism is associated with exercise-mediated changes of insulin resistance in healthy men. Metabolism 52: 209–212 (2003)

    Article  CAS  PubMed  Google Scholar 

  27. 27.

    Dedoussis GV, Kalioka AC, Psarras S, Chiou A, Mylona A, Papadopoulos NG, Andrikopoulos NK. Antiatherogenic effect of Pistacia lentiscus via GSH restoration and downregulation of CD36 mRNA expression. Atherosclerosis 174: 293–303 (2004)

    Article  CAS  PubMed  Google Scholar 

  28. 28.

    Sakamoto S. Case study: exercise therapy. Kyorin-shoin, Tokyo, pp. 86–167 (2000)

    Google Scholar 

  29. 29.

    Holloszy JO, Skinner JS, Toro G, Cureton TK. Effects of a six month program of endurance exercise on the serum lipids of middle-aged men. Am. J. Cardiol. 14: 753–760 (1964)

    Article  CAS  PubMed  Google Scholar 

  30. 30.

    Coll T, Rodriguez-Calvo R, Barroso E, Serrano L, Eyre E, Palomer X, Vazquez-Carrera M. Peroxisome proliferator-activated receptor (PPAR)β/δ: A new potential therapeutic target for the treatment of metabolic syndrome. Curr. Mol. Pharmacol. 2: 46–55 (2009)

    Article  CAS  PubMed  Google Scholar 

  31. 31.

    Castellano J, Guinda A, Delgado T, Rada M, Cayuela JA. Biochemical basis of the antidiabetic activity of oleanolic acid and related pentacyclic triterpenes. Diabetes 62: 1791–1799 (2013)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. 32.

    Borghouts LB, Keizer HA. Exercise and insulin sensitivity: a review. Int. Sports Med. 21: 1–12 (2000)

    Article  CAS  Google Scholar 

  33. 33.

    Goodyear LJ, Kahn BB. Exercise, glucose transport, and insulin sensitivity. Ann. Rev. Med. 49: 235–261 (1998)

    Article  CAS  PubMed  Google Scholar 

  34. 34.

    Gulve EA, Spina RJ. Effect of 7–10 days of cycle ergometer exercise on skeletal muscle GLUT-4 protein content. J. Appl. Physiol. 79: 1562–1566 (1995)

    Article  CAS  PubMed  Google Scholar 

  35. 35.

    Hardin DS, Azzarelli B, Edwards J, Wigglesworth J, Maianu L, Brechtel G, Johnson A, Baron A, Garvey WT. Mechanisms of enhanced insulin sensitivity in endurance-trained athletes: effects on blood flow and differential expression of GLUT-4 in skeletal muscles. J. Clin. Endocrinol. Metabolism. 80: 2437–2446 (1995)

    CAS  Google Scholar 

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We thank the General Manager of the Greek-Japanese Chamber of Commerce in Athens, Ms. Katerina Katopis, for initiating this study. We also thank the Chios Mastic Growers Association in Greece, particularly its President Mr. George Toumpos, for providing moral support for this study, and the following people for their role in supporting academic and cultural exchange between Japan and Greece: Ambassador of Greece to Japan, Ambassador Loukas Karatsolis; the former Ambassador of Greece to Japan, Ambassador Nikolaos Tsamados; the Counsellor of the Greek Embassy in Tokyo, Mr. Dionyssios Protopapas; the Ambassador of Japan to Greece, Ambassador Masuo Nishibayashi; and the former Ambassador of Japan to Greece, Ambassador Hiroshi Toda. The authors received no financial or material support for this research.

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Correspondence to Tomoko Fukazawa.

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Fukazawa, T., Smyrnioudis, I., Konishi, M. et al. Effects of Chios mastic gum and exercise on physical characteristics, blood lipid markers, insulin resistance, and hepatic function in healthy Japanese men. Food Sci Biotechnol 27, 773–780 (2018). https://doi.org/10.1007/s10068-018-0307-3

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  • Chios mastic gum
  • Physical activity
  • Triglycerides
  • Insulin