Food Science and Biotechnology

, Volume 27, Issue 2, pp 353–366 | Cite as

Enzymatic synthesis of structured lipids with behenic acid at the sn-1, 3 positions of triacylglycerols

  • Wai-Ming Kok
  • Cheng-Hock Chuah
  • Sit-Foon Cheng


A long chain saturated fatty acid (SFA), behenic acid, is incorporated into the sn-1, 3 positions of triacylglycerols in palm olein (POo) and high-oleic sunflower oil (HOS) by solvent-free interesterification catalyzed by Lipozyme RM IM. The enzymatic interesterified HOS (EIE-HOS) yielded 76.5% of BOO and BOB as compared to 45.6% in POo (EIE-POo). The sn-2 position of EIE-HOS displayed 5.3 mol% of SFA which is significantly lower compared to 13.5 mol% in EIE-POo (P < 0.001). The sn-1, 3 positions of EIE-POo exhibited greater amount of behenic acid (82.0 mol%) in relation to EIE-HOS (64.0 mol%) (P < 0.001). Due to the greater variety of constitutive triacylglycerol, EIE-POo showed greater differences between onset (To) and offset temperature (Tf) in the melting endotherms (76.99 °C) as compared to EIE-HOS (68.65 °C), and may offer more intensive cooling sensation and flavor release.


Enzymatic interesterification reaction Structured lipids Behenic acid sn-1, 3 positions Lipozyme RM IM 



The present study was supported by University of Malaya, Kuala Lumpur, Malaysia, through University Malaya Research Grant (RG274-14AF) and Malaysian Ministry of Education’s Fundamental Research Grant Scheme (FRGS) (FP023-2014B). The authors have no conflicts of interest to declare.


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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  1. 1.Unit of Research on Lipids (URL), Department of Chemistry, Faculty of ScienceUniversity of MalayaKuala LumpurMalaysia

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