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Enzymatic synthesis of structured lipids with behenic acid at the sn-1, 3 positions of triacylglycerols

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Abstract

A long chain saturated fatty acid (SFA), behenic acid, is incorporated into the sn-1, 3 positions of triacylglycerols in palm olein (POo) and high-oleic sunflower oil (HOS) by solvent-free interesterification catalyzed by Lipozyme RM IM. The enzymatic interesterified HOS (EIE-HOS) yielded 76.5% of BOO and BOB as compared to 45.6% in POo (EIE-POo). The sn-2 position of EIE-HOS displayed 5.3 mol% of SFA which is significantly lower compared to 13.5 mol% in EIE-POo (P < 0.001). The sn-1, 3 positions of EIE-POo exhibited greater amount of behenic acid (82.0 mol%) in relation to EIE-HOS (64.0 mol%) (P < 0.001). Due to the greater variety of constitutive triacylglycerol, EIE-POo showed greater differences between onset (To) and offset temperature (Tf) in the melting endotherms (76.99 °C) as compared to EIE-HOS (68.65 °C), and may offer more intensive cooling sensation and flavor release.

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Acknowledgements

The present study was supported by University of Malaya, Kuala Lumpur, Malaysia, through University Malaya Research Grant (RG274-14AF) and Malaysian Ministry of Education’s Fundamental Research Grant Scheme (FRGS) (FP023-2014B). The authors have no conflicts of interest to declare.

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  1. Unit of Research on Lipids (URL), Department of Chemistry, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Wai-Ming Kok, Cheng-Hock Chuah & Sit-Foon Cheng

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  1. Wai-Ming Kok
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  2. Cheng-Hock Chuah
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Correspondence to Sit-Foon Cheng.

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Kok, WM., Chuah, CH. & Cheng, SF. Enzymatic synthesis of structured lipids with behenic acid at the sn-1, 3 positions of triacylglycerols. Food Sci Biotechnol 27, 353–366 (2018). https://doi.org/10.1007/s10068-017-0271-3

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  • DOI: https://doi.org/10.1007/s10068-017-0271-3

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