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Antioxidant and antimicrobial activities of fresh garlic and aged garlic by-products extracted with different solvents

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Abstract

This study aims to investigate antioxidative and antibacterial properties of fresh garlic (non-aged, NG) and aged garlic (AG) by-products extracted with distilled water, ethanol, or chloroform. To determine their antioxidative and antibacterial capacities, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assay, and H2O2 radical scavenging activity, Fe2+ chelating activity, total ferric reducing antioxidant power (FRAP), and disc diffusion tests were performed. Total phenol and flavonoid contents from distilled water extract of AG were significantly higher than those of NG. DPPH, ABTS, FRAP, and H2O2 scavenging activities of distilled water extract of AG were higher than those of NG. However, Fe2+ chelating activities of ethanol and chloroform extracts were higher than those of distilled water extracts for both NG and AG. Antibacterial effects of AG were higher than those of NG. In conclusion, aged garlic showed more potent antioxidant and antibacterial effects than fresh garlic.

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References

  1. Saravanan G, Prakash J. Effect of garlic (Allium sativum) on lipid peroxidation in experimental myocardial infarction in rats. J Ethnopharmacol. 94(1):155–158 (2004)

    Article  CAS  Google Scholar 

  2. Ide N, Lau BH. Garlic Compounds Protect Vascular Endothelial Cells from Oxidized Low Density Lipoprotein-induced Injury. J. Pharm. Pharmacol. 49(9):908–911 (1997)

    Article  CAS  Google Scholar 

  3. Amagase H, Petesch BL, Matsuura H, Kasuga S, Itakura Y. Recent advances on the nutritional effects associated with the use of garlic as a supplement: intake of garlic and its bioactive components. J. Nutr. 131(3):955S–962S (2001)

    Article  CAS  Google Scholar 

  4. Rose P, Whiteman M, Moore PK, Zhu YZ. Bioactive S-alk(en)yl cysteine sulfoxide metabolites in the genus Allium: the chemistry of potential therapeutic agents. Nat Prod Rep. 22(3):351–368 (2005)

    Article  CAS  Google Scholar 

  5. Song, K, Milner JA. Recent Advances on the Nutritional Effects Associated with the Use of Garlic as a Supplement: The Influence of Heating on the Anticancer Properties of Garlic. J. Nutr. 131(3):1054S–1057S (2001)

    Article  CAS  Google Scholar 

  6. Kim JS, Kang OJ, Gweon OC. Comparison of phenolic acids and flavonoids in black garlic at different thermal processing steps. J Funct Foods. 5(1):80–86 (2013)

    Article  CAS  Google Scholar 

  7. Shin JH, Choi DJ, Chung MJ, Kang MJ, Sung NJ. Changes of Physicochemical Components and Antioxidant Activity of Aged Garlic at Different Temperatures. J Korean Soc Food Sci Nutr. 37(9):1174–1181 (2008)

    Article  CAS  Google Scholar 

  8. Wang SJ, Lee JH, Choi MJ, Lee SK. Effects of aged black garlic extracts on the rheology of flour dough. J Korean Soc Food Sci Nutr. 41(3):430–435 (2012)

    Article  CAS  Google Scholar 

  9. Lee HH, Kim IJ, Kang ST, Kim YH, Lee JO, Ryu CH. Development of black garlic Yakju and its antioxidant activity. Korean J. Food Sci. Technol. 42(1): 69–74 (2010)

    CAS  Google Scholar 

  10. Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Meth. Enzymol. 299:152–178 (1999)

    Article  CAS  Google Scholar 

  11. Arvouet-Grand A, Vennat B, Pourrat A, Legret P. Standardization of propolis extract and identification of principal constituents. J Pharm Belg. 49(6):462–468 (1994)

    CAS  Google Scholar 

  12. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 181(4617):1199–1200 (1958)

    Article  CAS  Google Scholar 

  13. Re P, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26(9):1231–1237 (1999)

    Article  CAS  Google Scholar 

  14. Oyaizu, M. Studies on product of browning reaction prepared from glucose amine. Japanese Journal of Nutrition 44:307–315 (1986)

    Article  CAS  Google Scholar 

  15. Dinis TCP, Madeira VMC, Almeida LM. Action of phenolic derivates (acetoaminophen, salycilate, and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch. Biochem. Biophy. 315:161–169 (1994)

    Article  CAS  Google Scholar 

  16. Okamoto G, Hayase F, Kato H. Scavenging of active oxygen species by glycated proteins. Biosci. Biotechnol. Biochem. 56(6):928–931 (1992)

    Article  CAS  Google Scholar 

  17. Meena MR, Sethi VIJAY. Antimicrobial activity of essential oils from spices. J Food Sci. Technol. 31(1):68–70 (1994)

    CAS  Google Scholar 

  18. Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 Chinese medicinal plants associated with anticancer. Life Sci. 74(17):2157–2184 (2004)

    Article  CAS  Google Scholar 

  19. Bozin B, Mimica-Dukic N, Samojlik I, Goran A, Igic R. Phenolics as antioxidants in garlic (Allium sativum L., Alliaceae). Food Chem. 111(4):925–929 (2008)

    Article  CAS  Google Scholar 

  20. Nencini C, Menchiari A, Franchi GG, Micheli L. In vitro antioxidant activity of aged extracts of some Italian Aliium species. Plant Foods Hum Nutr. 66(1):11–16 (2011)

    Article  CAS  Google Scholar 

  21. Colin-Gonzalez AL, Santana RA, Silva-Islas CA, Chanez-Cardenas ME, Santamaria A, Maldonado PD. The antioxidant mechanisms underlying the aged garlic extract- and S-allyl cysteine-induced protection. Oxid Med Cell Longev. 2012:16 (2012)

    Article  Google Scholar 

  22. Bae SE, Cho SY, Won YD, Lee SH, Park HJ. Changes in S-allyl cysteine contents and physicochemical properties of black garlic during heat treatment. Lebenson Wiss Technol. 55(1):397–402 (2014)

    Article  CAS  Google Scholar 

  23. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Lebenson Wiss Technol. 28(1):25–30 (1995)

    Article  CAS  Google Scholar 

  24. Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic. Biol. Med. 20(7):933–956 (1996)

    Article  CAS  Google Scholar 

  25. Martysiak-Żurowska D, Weronika W. A comparison of ABTS and DPPH methods for assessing the total antioxidant capacity of human milk. Acta Sci Pol Technol Aliment. 11(1):83–89 (2012)

    Google Scholar 

  26. Liu C, Yang X, Yao Y, Huang W, Sun W, Ma,Y. Determination of antioxidant activity in garlic (Allium sativum) extracts subjected to boiling process in vitro. Journal of Food and Nutrition Research 2(7):383–387 (2014)

    Article  Google Scholar 

  27. Yin MC, Cheng WS. Antioxidant and antimicrobial effects of four garlic-derived organosulfur compounds in ground beef. Meat Sci. 63(1):23–28 (2003)

    Article  Google Scholar 

  28. Saha B, Iglesias M, Dimming IW, Streat M. Sorption of trace heavy metals by thiol containing chelating resins. Solvent Extraction and Ion Exchange 18(1):133–167 (2000)

    Article  CAS  Google Scholar 

  29. Ide N, Matsuura H, Itakura Y. Scavenging effect of aged garlic extract and its constituents on active oxygen species. Phytother Res. 10(4):340–341 (1996)

    Article  Google Scholar 

  30. Ide N, Lau BHS, Ryu K, Matsuura H, Itakura Y. Antioxidant effects of fructosyl arginine, a Maillard reaction product in aged garlic extract. J. Nutr. Biochem. 10(6):372–376 (1999)

    Article  CAS  Google Scholar 

  31. Dziri S, Hassen I, Fatnassi S, Mrabet Y, Casabianca H, Hanchi B, Hosni K. Phenolic constituents, antioxidant and antimicrobial activities of rosy garlic (Allium roseum var. odoratissimum). J Funct Foods. 4(2):423–432 (2012)

  32. Benkeblia N. Antimicrobial activityof essential oil extracts of various onions (Allium cepa) and garlic (Allium sativum). Lebenson Wiss Technol. 37(2):263–268 (2004)

    Article  CAS  Google Scholar 

  33. Alzoreky NS, Nakahara K. Antibacterial activity of extracts from some edible plants commonly consumed in Asia. Int J Food Microbiol. 80(3):223–230 (2003)

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by the Brain Korea 21 Plus Project of Department of Food Science and Biotechnology of Animal Resources, Konkuk University in 2017.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 143-701, Republic of Korea

    Hyun-Joo Jang, Hyun-Jin Lee, Dong-Kyu Yoon, Da-Som Ji, Ji-Han Kim & Chi-Ho Lee

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  1. Hyun-Joo Jang
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Correspondence to Chi-Ho Lee.

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Jang, HJ., Lee, HJ., Yoon, DK. et al. Antioxidant and antimicrobial activities of fresh garlic and aged garlic by-products extracted with different solvents. Food Sci Biotechnol 27, 219–225 (2018). https://doi.org/10.1007/s10068-017-0246-4

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  • DOI: https://doi.org/10.1007/s10068-017-0246-4

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