Effects of l-arginine on growth hormone and insulin-like growth factor 1

Abstract

l-Arginine has been reported to promote cellular and organismal growth. In this study, the effects of l-arginine on the expression of growth hormone (GH) and insulin-like growth factor 1 (IGF-1), the two key growth factors, are investigated in cultured GH3 pituitary epithelium and HepG2 cells, respectively. l-Arginine significantly induced the gene expression of GH and IGF-1 in GH3 pituitary epithelium and HepG2 hepatocytes respectively, and reduced IGF binding protein-1 gene expression in HepG2 cells assessed via quantitative polymerase chain reaction analysis. l-Arginine also significantly induced GH and IGF-1 hormone secretion from GH3 and HepG2 cells, respectively. In addition, the multi-target ELISA analysis conducted revealed that phosphorylation of p-38 MAPK, MEK, and JNK were significantly increased in HepG2 cells, suggesting l-arginine-induced activation of the MAPK signaling pathway. These results suggest that l-arginine promotes the synthesis and secretion of GH and IGF-1 in vitro and induces the MAPK signaling cascade in cultured hepatocytes.

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MSIP) (No. NRF-2016R1A2A2A05005483).

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Correspondence to Sung-Joon Lee.

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Oh, HS., Oh, S.K., Lee, J.S. et al. Effects of l-arginine on growth hormone and insulin-like growth factor 1. Food Sci Biotechnol 26, 1749–1754 (2017). https://doi.org/10.1007/s10068-017-0236-6

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Keywords

  • l-Arginine
  • Growth hormone
  • Insulin-like growth factor 1
  • Mitogen-activated protein kinase