Fabrication of an electrochemical biosensor with ZnO nanoflakes interface for methylglyoxal quantification in food samples

Abstract

Increased consumption of fried foods such as grilled chicken contains elevated levels of methylglyoxal (MG), which is associated with diabetes mellitus. Hence, in this work, glyoxalase 1(GLO 1) based, zinc oxide (ZnO) flakes interfaced mediator free electrochemical biosensor was developed to detect MG in grilled chicken. ZnO flakes were synthesized by direct precipitation method. X-ray diffractometer and field emission scanning electron microscope were used to study the structural and morphological characteristics of ZnO flakes. The immobilization of GLO 1 on Pt/ZnO flakes modified electrode was confirmed by Fourier transform infrared spectroscopy. Cyclic voltammetric and amperometric studies were carried out using Pt/ZnO flakes/GLO 1 working electrode. The developed biosensor exhibited linear range of 0.6–2.0 µM, sensitivity of 0.281 µA µM−1, LOD of 9 nM with a response time of <4 s and shelf life of 18 days (89%).

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Acknowledgements

The authors are grateful to the Department of Science and Technology, New Delhi, for their financial support (DST/TSG/PT/2008/28, SR/FST/ETI-284/2011(C) and (Nano Mission Council (No. SR/NM/PG-16/2007)). We also acknowledge SASTRA University, Thanjavur for extending infrastructural support to carry out the study.

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Correspondence to John Bosco Balaguru Rayappan.

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Jayaprakasan, A., Thangavel, A., Ramachandra Bhat, L. et al. Fabrication of an electrochemical biosensor with ZnO nanoflakes interface for methylglyoxal quantification in food samples. Food Sci Biotechnol 27, 9–17 (2018). https://doi.org/10.1007/s10068-017-0193-0

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Keywords

  • Biosensor
  • ZnO flakes
  • Human glyoxalase 1
  • Methylglyoxal
  • Cyclic voltammetry
  • Amperometry