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Anti-inflammatory and anti-genotoxic activity of branched chain amino acids (BCAA) in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages

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Abstract

The purpose of this study was to evaluate the anti-inflammatory and anti-genotoxic activity of branched-chain amino acids (BCAAs) in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages. BCAAs inhibited LPS-induced NO production, with 100 mM leucine having the most pronounced effect, suppressing NO production by 81.15%. Valine and isoleucine also reduced NO production by 29.65 and 42.95%, respectively. Furthermore, BCAAs suppressed the inducible nitric oxide synthase mRNA expression. Additionally, BCAAs decreased the mRNA expression of interleukin-6 and cyclooxygenase-2 which are proinflammatory mediators. Anti-genotoxic activities of BCAAs were assessed using the alkaline comet assay and valine, isoleucine, and leucine significantly (p < 0.05) decreased tail length of DNA (damaged portion) to 254.8 ± 7.5, 235.6 ± 5.6, and 271.5 ± 19.9 μm compared than positive control H2O2 (434.3 ± 51.3 μm). These results suggest that BCAAs can be used in the pharmaceutical or functional food industries as anti-inflammatory agents or anti-cancer agents.

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Acknowledgements

This research was supported by the High Value-added Food Technology Development Program (313021-3) of the Ministry of Agriculture, Food and Rural Affairs (Korea).

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Authors and Affiliations

  1. Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul, 05029, Korea

    Jae Hoon Lee, Hyue Ju Jin & Hyun-Dong Paik

  2. Department of Food and Nutrition, Kyungnam University, Changwon, 51767, Korea

    Eunju Park & Yunjeong Lee

  3. Department of Food Science and Technology, Department of Interdisciplinary Bio IT Materials, Seoul National University of Science and Technology, Seoul, 01811, Korea

    Seung Jun Choi

  4. R&D Center, Daesang Corporation, Icheon, 17384, Korea

    Gyu Whan Lee

  5. Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Korea

    Pahn-Shick Chang

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  1. Jae Hoon Lee
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Lee, J.H., Park, E., Jin, H.J. et al. Anti-inflammatory and anti-genotoxic activity of branched chain amino acids (BCAA) in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages. Food Sci Biotechnol 26, 1371–1377 (2017). https://doi.org/10.1007/s10068-017-0165-4

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  • DOI: https://doi.org/10.1007/s10068-017-0165-4

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