Mixed seeds juice with high antioxidant capacity and digestive enzyme activity and its application

Abstract

To investigate the synergistic and combined effects of a seed–vegetable combination, oilseed crops (safflower seed, evening primrose seed, and sesame seed) and cereals (whole buckwheat grain) were extracted with carrot using a low-speed juice extractor. Nutrient composition showed that the mixed seeds juice provided a well-balanced diet to supplement nutrient deficiency. Four assays of antioxidant activities showed that all seed juices exhibited higher activities than carrot juice. Similarly, mixed seeds juice displayed the highest α-amylase and protease activities, implicating the formulation in the improvement of the relatively low digestibility of seed. These results indicated that juice extraction significantly (p<0.001) influenced the nutrient contents, antioxidant activities, and digestion enzyme activities of seed juice. The synergistic and combined interactions of seeds and vegetables may be beneficial in enhancing biological functions and result in a well-balanced diet.

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References

  1. 1.

    Wang S, Meckling KA, Marcone MF, Kakuda Y, Tsao R. Synergistic, additive, and antagonistic effects of food mixtures on total antioxidant capacities. J. Agr. Food Chem. 59: 960–968 (2011)

    CAS  Article  Google Scholar 

  2. 2.

    Hotta Y, Nagatsu A, Liu W, Muto T, Narumiya C, Lu X, Yajima M, Ishikawa N, Miyazeki K, Kawai N. Protective effects of antioxidative serotonin derivatives isolated from safflower against postischemic myocardial dysfunction. Mol. Cell. Biochem. 238: 151–162 (2002)

    CAS  Article  Google Scholar 

  3. 3.

    Palter R, Lundin RE. A bitter principle of safflower; Matairesinol monoglucoside. Phytochemistry 9: 2407–2409 (1970)

    CAS  Article  Google Scholar 

  4. 4.

    Quettier-Deleu C, Gressier B, Vasseur J, Dine T, Brunet C, Luyckx M, Cazin M, Cazin JC, Bailleul F, Trotin F. Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J. Ethnopharmacol. 72: 35–42 (2000)

    CAS  Article  Google Scholar 

  5. 5.

    Zhang ZL, Zhou ML, Tang Y, Li FL, Tang YX, Shao JR, Xue WT, Wu YM. Bioactive compounds in functional buckwheat food. Food Res. Int. 49: 389–395 (2012)

    CAS  Article  Google Scholar 

  6. 6.

    Anilakumar KR, Pal A, Khanum F, Bawa AS. Nutritional, medicinal and industrial uses of sesame (Sesamum indicum L.) seeds -An overview. Agric. Conspec. Sci. 75: 159–168 (2010)

    Google Scholar 

  7. 7.

    Stonemetz D. A review of the clinical efficacy of evening primrose. Holist. Nurs. Pract. 22: 171–174 (2008)

    Article  Google Scholar 

  8. 8.

    Simon PW, Freeman RE, Vieira JV, Boiteux LS, Briard M, Nothnagel T, Michalik B, Kwon YS. Carrot. Vegetables II. Handbook of Plant Breeding. 2. Springer, New York, NY, USA. pp. 327–357 (2008)

  9. 9.

    Choi MH, Kim MJ, Jeon YJ, Shin HJ. Quality changes of fresh vegetable and fruit juice by various juicers. Korean Soc. Biotechnol. Bioeng. J. 29: 145–154 (2014)

    Google Scholar 

  10. 10.

    Iwe MO. Handbook of sensory methods and analysis. Rojoint Communication Services Ltd., Enugu, Nigeria. pp. 7-12 (2002)

  11. 11.

    MFDS. Korean Food Standards Codex. Available from: http://www. foodsafetykorea.go.kr/foodcode/index.jsp. Accessed Mar. 19, 2015.

  12. 12.

    Martins-Júnior HA, Wang AY, Alabourda J, Pires MA, Vega OB, Lebre DT. A validated method to quantify folic acid in wheat flour samples using liquid chromatography-tandem mass spectrometry. J. Brazil. Chem. Soc. 19: 971–977 (2008)

    Article  Google Scholar 

  13. 13.

    Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 26: 1199–1200 (1958)

    Article  Google Scholar 

  14. 14.

    Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio. Med. 26: 1231–1237 (1999)

    CAS  Article  Google Scholar 

  15. 15.

    Marcocci L, Packer L, Droy-Lefaix MT, Sekaki A, Gardes-Albert M. Antioxidant action of Ginkgo biloba extract EGb 761. Method. Enzymol. 234: 462–475 (1994)

    CAS  Article  Google Scholar 

  16. 16.

    Elarbi MB, Khemiri H, Jridi T, Hamida JB. Purification and characterization of a-amylase from safflower (Carthamus tinctorius L.) germinating seeds. C. R. Biol. 332: 426–432 (2009)

    Article  Google Scholar 

  17. 17.

    Oh DG, Jang YK, Woo JE, Kim JS, Lee CH. Metabolomics reveals the effect of garlic on antioxidant-and protease-activity during cheonggukjang (fermented soybean paste) fermentation. Food Res. Int. 82: 86–94 (2016)

    CAS  Article  Google Scholar 

  18. 18.

    Madaan N, Mudgal V, Mishra S, Srivastava A, Singh R. Studies on biochemical role of accumulation of heavy metals in Safflower. Open Nutraceuticals J. 4: 199–204 (2011)

    CAS  Article  Google Scholar 

  19. 19.

    Yu SY, Lee YJ, Kang SN, Lee SK, Jang JY, Lee HK, Lim JH, Lee OH. Analysis of food components of Carthamus tinctorius L. seed and its antimicrobial activity. Korean J. Food Preserv. 20: 227–233 (2013)

    Google Scholar 

  20. 20.

    Pyo YH, Ahn MS, Yim UK. Effects of tocopherols on the oxidation stability of evening primrose oil. J. Food Sci. Tech. Mys. 22: 255–260 (1990)

    CAS  Google Scholar 

  21. 21.

    Yoshida H, Takagi S. Effects of seed roasting temperature and time on the quality characteristics of sesame (Sesamum indicum) oil. J. Sci. Food Agr. 75: 19–26 (1997)

    CAS  Article  Google Scholar 

  22. 22.

    Grundy SM, Denke MA. Dietary influences on serum lipids and lipoproteins. J. Lipid Res. 31: 1149–1172 (1990)

    CAS  Google Scholar 

  23. 23.

    Yu SY, Lee YJ, Kim JD, Kang SN, Lee SK, Jang JY, Lee HK, Lim JH, Lee OH. Phenolic composition, antioxidant activity and anti-adipogenic effect of hot water extract from safflower (Carthamus tinctorius L.) seed. Nutrients 5: 4894–4907 (2013)

    Article  Google Scholar 

  24. 24.

    Vishwanath H, Anilakumar K, Harsha S, Khanum F, Bawa A. In vitro antioxidant activity of Sesamum indicum seeds. Asian J. Pharm. Clin. Res. 5: 56–60 (2012)

    Google Scholar 

  25. 25.

    Swain RR, Dekker EE. Seed germination studies. I. Purification and properties of an a-amylase from the cotyledons of germinating peas. Biochim. Biophys. Acta 122: 75–86 (1966)

    CAS  Google Scholar 

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Correspondence to Jeong Su Jang.

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Park, SH., Park, HJ., Kim, JY. et al. Mixed seeds juice with high antioxidant capacity and digestive enzyme activity and its application. Food Sci Biotechnol 26, 237–244 (2017). https://doi.org/10.1007/s10068-017-0032-3

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Keywords

  • juice
  • mixed seeds juice
  • antioxidant activity
  • nutritional composition
  • enzyme activity