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Anti-oxidative and anti-inflammatory activities of devil’s club (Oplopanax horridus) leaves

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Abstract

This study aimed to investigate the anti-oxidative properties of the ethanolic extracts of the devil’s club (Oplopanax horridus) leaves, stems, and roots. Furthermore, the anti-inflammatory activity of the leaf extract was analyzed. The leaf extract had higher total phenolic and flavonoid contents and anti-oxidative activity (radical scavenging, reducing power, and inhibition of lipid oxidation) than the root and stem extracts. The leaf extract also had anti-inflammatory effects. It significantly reduced lipopolysaccharide (LPS)-induced nitric oxide (NO; 71.0% at 50 μg/mL), tumor necrosis factor (TNF)-α (87.6% at 100 μg/mL), and interleukin (IL)-6 (36.2% at 100 μg/mL) production in murine RAW 264.7 macrophages. Furthermore, LPS-induced inducible nitric oxide synthase (iNOS) expression was decreased by the leaf extract (IC50=24.4 μg/mL). The ultra performance liquid chromatography-diode array detector (UPLC-DAD) analysis showed that the leaf extract contained gallic acid, protocatechuic acid, chlorogenic acid, and maltol. These findings suggest that the leaf extract could be utilized as a functional food material because of its anti-oxidative and anti-inflammatory activities.

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Authors and Affiliations

  1. Division of Strategic Food Research, Korea Food Research Institute, Seongnam, Gyeonggi, 13539, Korea

    Mi Jang, Young-Chul Lee, Hee-Do Hong, Young Kyoung Rhee, Tae-Gyu Lim, Kyung-Tack Kim & Chang-Won Cho

  2. Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University, Yongin, Gyeonggi, 17104, Korea

    Mi Jang

  3. Department of Food, Nutrition, and Packaging Sciences, Clemson University, Clemson, SC, 29634, USA

    Feng Chen

  4. Division of Applied Life Sciences (BK21 plus)/Department of Food Science & Technology, and Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Gyeongnam, 52828, Korea

    Hyun-Jin Kim

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  1. Mi Jang
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  2. Young-Chul Lee
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Correspondence to Chang-Won Cho.

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Jang, M., Lee, YC., Hong, HD. et al. Anti-oxidative and anti-inflammatory activities of devil’s club (Oplopanax horridus) leaves. Food Sci Biotechnol 26, 213–220 (2017). https://doi.org/10.1007/s10068-017-0029-y

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  • DOI: https://doi.org/10.1007/s10068-017-0029-y

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