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Antioxidant activities of honeybee products and their mixtures

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Abstract

In this study, the total phenolic content (TPC), antioxidant activity, and free radical scavenging activities (FRSA) of 70 samples comprising honeybee products (honey, pollen, royal jelly, and propolis) and their mixtures were determined. The TPC was determined in accordance with the Folin–Ciocalteu method, antioxidant activity with phosphomolybdenum, and FRSA with the 1,1-diphenyl-2-picryl hydrazyl (DPPH) assays. Honeybee propolis showed the greatest TPC, antioxidant activity, and FRSA followed by pollen, honey, and royal jelly, respectively. A positive correlation was observed between TPC and antioxidant activity of honey, pollen and mixed samples (respectively, r=0.91, r=0.93 and r=0.92) (p<0.01). Similarly, honey and mixed samples exhibited positive correlations with TPC and FRSA (respectively, r=0.98 and r=0.92) (p<0.01). It was concluded that honeybee products and their mixtures have antioxidant activity and FRSA and these effects may be attributed to their phenolic content.

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References

  1. Halliwell B, Gutteridge JMC. The antioxidant of human extracellular fluids. Arch. Biochem. Biophys. 280: 1–8 (1990)

    Article  CAS  Google Scholar 

  2. Nagai T, Sakai M, Inoue R, Inoue H, Suzuki N. Antioxidative activities of some commercially honeys, royal jelly, and propolis. Food Chem. 5: 237–240 (2001)

    Article  Google Scholar 

  3. Koc AN, Silici S, Kasap F, Oz H, Mavus-Buldu H, Ercal BD. Antifungal activity of the honeybee products against Candida spp. and Trichosporon spp. J. Med. Food 14: 128–134 (2011)

    Article  Google Scholar 

  4. Kanbur M, Eraslan G, Silici S. Antioxidant effect of propolis against exposure to propetamphos in rats. Ecotox. Environ. Safe. 72: 909–915 (2009)

    Article  CAS  Google Scholar 

  5. Silici S, Ekmekcioglu O, Eraslan G, Demirtas A. Antioxidative effect of royal jelly in cisplatin induced testes damage. Urology 74: 545–551 (2009)

    Article  Google Scholar 

  6. Bogdanov S. Nature and origin of the antibacterial substances in honey. LWTFood Sci. Technol. 30: 748–753 (1997)

    CAS  Google Scholar 

  7. Gheldof N, Engeseth NJ. Antioxidant capacity of honeys from various floral sources based on the determination of oxygen radical absorbance capacity and inhibition of in vitro lipoprotein oxidation in human serum samples. J. Agr. Food. Chem. 50: 3050–3055 (2002)

    Article  CAS  Google Scholar 

  8. Al-Mamary M, Al-Meeri A, Al-Habori M. Antioxidant activities and total phenolics of different types of honey. Nutr. Res. 22: 1041–1047 (2002)

    Article  CAS  Google Scholar 

  9. Ferreres F, Garcia-Viguera C, Tomas-Lorente F, Tomas-Barberan FA. Hesperetin: A marker of the floral origin of citrus honey. J. Agr. Food. Chem. 61: 121–123 (1993)

    Article  CAS  Google Scholar 

  10. Haydak MH. Honey bee nutrition. Annu. Rev. Entomol. 15: 143–156 (1970)

    Article  Google Scholar 

  11. Marcucci MC, Ferreres F, Garcia-Viguera C, Bankova VS, de Castro SL, Dantas AP, Valente PH, Paulino N. Phenolic compounds from Brazilian propolis with pharmacological activities. J. Ethnopharmacol. 74: 105–112 (2001)

    Article  CAS  Google Scholar 

  12. Greenaway W, May J, Scaysbrook T, Whatley FR. Identification by gas chromatography-mass spectrometry of 150 compounds in propolis. Z. Naturforsch. C 46: 111–121 (1991)

    CAS  Google Scholar 

  13. Yonar ME, Yonar SM, Pala, A, Silici S, Saglam N. Trichlorfon-induced haematological and biochemical changes in Cyprinus carpio: Ameliorative effect of propolis. Dis. Aquat. Organ. 114: 209–2016 (2015)

    Article  CAS  Google Scholar 

  14. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. Am. J. Enol. Viticult. 16: 144–158 (1965)

    CAS  Google Scholar 

  15. Silici S, Sagdic O, Ekici L. Total phenolic content, antiradical, antioxidant and antimicrobial activities of Rhododendron honeys. Food Chem. 121: 238–243 (2010)

    Article  CAS  Google Scholar 

  16. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formulation of a phosphomolybdenum complex: Specific application of vitamin E. Anal. Biochem. 269: 337–341 (1999)

    Article  CAS  Google Scholar 

  17. Gyamfi MA, Yonamine M, Aniya Y. Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally-induced liver injuries. Gen. Pharmacol. 32: 661–667 (1999)

    Article  CAS  Google Scholar 

  18. Estevinho L, Pereira AP, Moreira L, Dias LG, Pereira E. Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey. Food Chem. Toxicol. 46: 3774–3779 (2008)

    Article  CAS  Google Scholar 

  19. Pichichero E, Canuti L, Canini A. Characterisation of the phenolic and flavonoid fractions and antioxidant power of Italian honeys of different botanical origin. J. Sci. Food Agr. 89: 609–616 (2009)

    Article  CAS  Google Scholar 

  20. Dobre I, Gadei G, Patrascu L, Elisei AM, Segal R. The antioxidant activity of selected Romanian honeys. The Annals of the University Dunarea de Jos of Galati Fascicle VI, Food Technol. 34: 67–73 (2010)

    CAS  Google Scholar 

  21. Bertoncelj J, Dobersek U, Jamnik M, Golob T. Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey. Food Chem. 105: 822–828 (2007)

    Article  CAS  Google Scholar 

  22. Fatrcova-Sramkova K, Nazkova J, Kacaniova M, Mariassyova M, Rovna K, Stricik M. Antioxidant and antimicrobial properties of monofloral bee pollen. J. Environ. Sci. Heal. B 48: 133–138 (2013)

    Article  CAS  Google Scholar 

  23. Leja M, Mareczek A, Wyzgolik G, Klepacz-Baniak J, Czekonska K. Antioxidative properties of bee pollen in selected plant species. Food Chem. 100: 237–240 (2007)

    Article  CAS  Google Scholar 

  24. Kroyer G, Hegedus N. Evaluation of bioactive properties of pollen extracts as functional dietary food supplement. Innov. Food Sci. Emerg. 2: 171–174 (2001)

    Article  CAS  Google Scholar 

  25. Morais M, Moreira L, Feas X, Estevinho LM. Honeybee-collected pollen from five Portuguese Natural Parks: Palynological origin, phenolic content, antioxidant properties and antimicrobial activity. Food Chem. Toxicol. 49: 1096–1101 (2011)

    Article  CAS  Google Scholar 

  26. Mejias E, Montenegro G. The antioxidant activity of Chilean honey and bee pollen produced in the Llaima Volcano’s zones. J. Food Quality 35: 315–322 (2012)

    Article  CAS  Google Scholar 

  27. Le Blanc BW, Davis OK, Boue S, De Lucca A, Deeby T. Antioxidant activity of Sonoran Desert bee pollen. Food Chem. 115: 1299–1305 (2009)

    Article  Google Scholar 

  28. Marghitas LA, Stanciu OG, Dezmirean DS, Popescu O, Bogdanov S, Campos M. In vitro antioxidant capacity of honeybee-collected pollen of selected floral origin harvested from Romania. Food Chem. 115: 878–883 (2009)

    Article  CAS  Google Scholar 

  29. Guo H, Kozuma Y, Yonekura M. Isolation and properties of antioxidative peptides fom water-soluble royal jelly protein hydrolysate. Food Sci. Technol. Res. 11: 222–230 (2005)

    Article  CAS  Google Scholar 

  30. Eraslan G, Kanbur M, Silici S, Altýnordulu S, Karabacak M. Effects of Cypermethrin on some biochemical changes in rats: The protective role of propolis. Exp. Anim. Tokyo 57: 453–460 (2008)

    Article  CAS  Google Scholar 

  31. Kumazawa S, Hamasaka T, Nakayama T. Antioxidant activity of propolis of various geographic origins. Food Chem. 84: 329–339 (2004)

    Article  CAS  Google Scholar 

  32. Gulcin I, Bursal E, Sehitoglu MH, Bilsel M, Goren AC. Polyphenol contents and antioxidant activity of lyophilized aqueus extract of propolis from Erzurum, Turkey. Food Chem. Toxicol. 48: 2227–2238 (2010)

    Article  Google Scholar 

  33. Moreira L, Dias LD, Pereira JA, Estevinho L. Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal. Food Chem. Toxicol. 46: 3482–3485 (2008)

    Article  CAS  Google Scholar 

  34. Buratti S, Bendetti S, Cosio MS. Evaluation of the antioxidant power of honey, propolis and royal jelly by amperometric flow injection analysis. Talanta 71: 1387–1392 (2007)

    Article  CAS  Google Scholar 

  35. Manthey JA, Grohmann K. Phenols in citrus peel byproducts. Concentrations of hydroxycinnamates and polymethoxylated flavones in citrus peel molasses. J. Agr. Food Chem. 49: 3268–3273 (2001)

    CAS  Google Scholar 

  36. Nagai T, Inoue R. Preparation and the functional properties of water extract and alkaline extract of royal jelly. Food Chem. 84: 181–186 (2004)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Erciyes University, Safiye Cikrikcioglu Vocational School, Kayseri, 38039, Turkey

    Duran Özkök

  2. Department of Agricultural Biotechnology, Erciyes University, Agriculture Faculty, Kayseri, 38039, Turkey

    Sibel Silici

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  1. Duran Özkök
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  2. Sibel Silici
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Correspondence to Duran Özkök.

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Özkök, D., Silici, S. Antioxidant activities of honeybee products and their mixtures. Food Sci Biotechnol 26, 201–206 (2017). https://doi.org/10.1007/s10068-017-0027-0

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  • DOI: https://doi.org/10.1007/s10068-017-0027-0

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