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Effect of 2,2-azobis (2-amidinopropane) dihydrochloride oxidized casein on the microstructure and microrheology properties of emulsions

Food Science and Biotechnology volume 25, pages 1283–1290 (2016)Cite this article

Abstract

The impacts of protein oxidation on the droplet size and microrheology properties of casein emulsions with 20% oil content were investigated. The degree of protein oxidation was indicated by carbonyl concentration. The droplets in the emulsions of different-oxidation-degree casein had bimodal distribution, but their size altered due to oxidation. The effects of protein oxidation on the morphology, motion type, viscoelasticity, and stability of droplets were also investigated by microrheology analysis. The droplet motion was blocked by protein oxidation due to mean square displacement slope results. Solid–liquid balance values provided the liquid behavior dominating these emulsions. Oxidation of carbonyl concentration 16.72 raised the primary droplets, increased the elasticity, decreased the viscosity, and promoted the droplet motion rate, resulting in better stability of emulsions. Further oxidation promoted the aggregation of droplets and resulted in poor stability.

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References

  1. Matemu AO, Kayahara H, Murasawa H, Katayama S, Nakamura S. Improved emulsifying properties of soy proteins by acylation with saturated fatty acids. Food Chem. 124: 596–602 (2011)

    CAS  Article  Google Scholar 

  2. Mession JL, Roustel S, Saurel R. Interactions in casein micelle -pea protein system (Part I): Heat-induced denaturation and aggregation. Food hydrocolloid. [Available online] (2015)

  3. Garnier C, Michon C, Durand S, Cuvelier G, Doublier JL, Launay B. Iotacarrageenan/casein micelles interactions: Evidence at different scales. Colloid. Surface. B 31: 177–184 (2003)

    CAS  Article  Google Scholar 

  4. Liu Y, Guo R. Interaction between Casein and the Oppositely Charged Surfactant. Biomacromolecules 8: 2902–2908 (2007)

    CAS  Article  Google Scholar 

  5. Dickinson E. Structure formation in casein-based gels, foams, and emulsions. Colloid. Surface. A 288: 3–11 (2006)

    CAS  Article  Google Scholar 

  6. Shao Y, Tang CH. Characteristics and oxidative stability of soy proteinstabilized oil-in-water emulsions: Influence of ionic strength and heat pretreatment. Food hydrocolloid. 37: 149–158 (2014)

    CAS  Article  Google Scholar 

  7. Berton C, Ropers MH, Bertrand D, Viau M, Genot C. Oxidative stability of oilin-water emulsions stabilised with protein or surfactant emulsifiers in various oxidation conditions. Food Chem. 131: 1360–1369 (2012)

    CAS  Article  Google Scholar 

  8. Qiu C, Zhao M, Decker EA, McClements DJ. Influence of protein type on oxidation and digestibility of fish oil-in-water emulsions: Gliadin, caseinate, and whey protein. Food Chem. 175: 249–257 (2015)

    CAS  Article  Google Scholar 

  9. Jung T, Hohn A, Grune T. The proteasome and the degradation of oxidized proteins: Part II-protein oxidation and proteasomal degradation. Redox Biol. 2C: 99–104 (2013)

    Google Scholar 

  10. Semagoto HM, Liu D, Koboyatau K, Hu J, Lu N, Liu X, Regenstein JM, Zhou P. Effects of UV induced photo-oxidation on the physicochemical properties of milk protein concentrate. Food Res. Int. 62: 580–588 (2014)

    CAS  Article  Google Scholar 

  11. Waraho T, McClements DJ, Decker EA. Mechanisms of lipid oxidation in food dispersions. Trends Food Sci. Tech. 22: 3–13 (2011)

    CAS  Article  Google Scholar 

  12. Stadtman ER. Protein oxidation and aging. Free Radical. Res. 257: 1250–1258 (2006)

    Article  Google Scholar 

  13. Wu W, Wu X, Hua Y. Structural modification of soy protein by the lipid peroxidation product acrolein. LWT-Food Sci. Technol. 43: 133–140 (2010)

    CAS  Article  Google Scholar 

  14. Chen N, Zhao M, Sun W, Ren J, Cui C. Effect of oxidation on the emulsifying properties of soy protein isolate. Food Res. Int. 52: 26–32 (2013)

    CAS  Article  Google Scholar 

  15. Wu W, Zhang C, Kong X, Hua Y. Oxidative modification of soy protein by peroxyl radicals. Food Chem. 116: 295–301 (2009)

    CAS  Article  Google Scholar 

  16. Sun C, Liu R, Wu T, Liang B, Shi C, Zhang M. Effect of superfine grinding on the structural and physicochemical properties of whey protein and applications for microparticulated proteins. Food Sci. Biotechnol. 24: 1637–1643 (2015)

    CAS  Article  Google Scholar 

  17. Tisserand C, Fleury M, Brunel L, Bru P, Meunier G. Passive microrheology for measurement of the concentrated dispersions stability. Prog. Coll. Pol. Sci. S. 139: 101–105 (2012)

    CAS  Google Scholar 

  18. Scheidegger D, Radici PM, Vergara-Roig VA, Bosio NS, Pesce SF, Pecora RP, Romano JC, Kivatinitz SC. Evaluation of milk powder quality by protein oxidative modifications. J. Dairy Sci. 96: 3414–3423 (2013)

    CAS  Article  Google Scholar 

  19. Liu Q, Lu Y, Han J, Chen Q, Kong B. Structure-modification by moderate oxidation in hydroxyl radical-generating systems promote the emulsifying properties of soy protein isolate. Food Struct. 6: 27–28 (2015)

    Article  Google Scholar 

  20. Ray M, Rousseau D. Stabilization of oil-in-water emulsions using mixtures of denatured soy whey proteins and soluble soybean polysaccharides. Food Res. Int. 52: 298–307 (2013)

    CAS  Article  Google Scholar 

  21. Slattery CW, Evard R. A model for the formation and structure of casein micelles from subunits of variable composition. Biochim. Biophys. Acta 317: 529–538 (1973)

    CAS  Article  Google Scholar 

  22. Dickinson E. Milk protein interfacial layers and relationship to the emulsion stability and rheology. Colloid. Surface. B 20: 197–210 (2001)

    CAS  Article  Google Scholar 

  23. Walstra P. Principles of emulsion formation. Chem. Eng. Sci. 48: 333–349 (1992)

    Article  Google Scholar 

  24. Liang Y, Wong SS, Pham SQ, Tan JJ. Effects of globular protein type and concentration on the physical properties and flow behaviors of oil-in-water emulsions stabilized by micellar casein–globular protein mixtures. Food Hydrocolloid. 54: 89–98 (2016)

    CAS  Article  Google Scholar 

  25. McCarthy NA, Kelly AL, O’Mahony JA, Fenelon MA. Sensitivity of emulsions stabilised by bovine ß-casein and lactoferrin to heat and CaCl2. Food Hydrocolloid. 35: 420–428 (2014)

    CAS  Article  Google Scholar 

  26. Liang Y, Patel H, Matia-Merino L, Ye A, Golding M. Structure and stability of heat-treated concentrated dairy-protein-stabilised oil-in-water emulsions: A stability map characterisation approach. Food Hydrocolloid. 33: 297–308 (2013)

    CAS  Article  Google Scholar 

  27. Moakes RJA, Norton ASIT. Preparation and characterisation of whey protein fluid gels: The effects of shear and thermal history. Food Hydrocolloid. 45: 227–235 (2015)

    CAS  Article  Google Scholar 

  28. Moschakis T, Murrayb BS, Dickinson E. On the kinetics of acid sodium casein ate g elation usin g particle t rackin g to p robe the m icrorheology. J. Colloid. Interf. Sci. 345: 278–285 (2010)

    CAS  Article  Google Scholar 

  29. Phoon PY, San Martin-Gonzalez MF, Narsimhan G. Effect of hydrolysis of soy ß-conglycinin on the oxidative stability of O/W emulsions. Food Hydrocolloid. 35: 429–443 (2014)

    CAS  Article  Google Scholar 

  30. Nishinari K, Fang Y, Guo S, Phillips GO. Soy proteins: A review on composition, aggregation and emulsificatio. Food Hydrocolloid. 39: 301–318 (2014)

    CAS  Article  Google Scholar 

  31. Hebishy E, Buffa M, Guamis B, Blasco-Moreno A, Trujillo AJ. Physical and oxidative stability of whey protein oil-in-water emulsions produced by conventional and ultra high-pressure homogenization: Effects of pressure and protein concentration on emulsion characteristics. Innov. Food Sci. Emerg. 32: 79–90 (2015)

    CAS  Article  Google Scholar 

  32. Liang HN, Tang CH. pH-dependent emulsifying properties of pea [Pisum sativum (L.)] proteins. Food Hydrocolloid. 33: 309–319 (2013)

    CAS  Article  Google Scholar 

  33. Yousfi M, Sebastien Alix, Lebeau M, Soulestin J, Lacrampe MF, Krawczak P. Evaluation of rheological properties of non-Newtonian fluids in micro rheology compounder: Experimental procedures for a reliable polymer melt viscosity measurement. Polym. Test. 40: 207–217 (2014)

    CAS  Article  Google Scholar 

  34. Sun C, Wu T, Liu R, Liang B, Tian Z, Zhang E, Zhang M. Effects of superfine grinding and microparticulation on the surface hydrophobicity of whey protein concentrate and its relation to emulsions stability. Food Hydrocolloid. 51: 512–518 (2015)

    CAS  Article  Google Scholar 

  35. Moschakis T. Microrheology and particle tracking in food gels and emulsions. Curr. Opin. Colloid In. 18: 311–323 (2013)

    CAS  Article  Google Scholar 

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Affiliations

  1. Key Laboratory of Food Nutrition and Safety (Tianjin University of Science & Technology), Ministry of Education, Tianjin, 300457, China

    Jianming Wang, Yaoyao Tan, Sisi Niu & Jinghua Yu

  2. College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, China

    Hui Xu, Sisi Niu & Jinghua Yu

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  1. Jianming Wang
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  2. Yaoyao Tan
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  3. Hui Xu
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  4. Sisi Niu
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  5. Jinghua Yu
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Correspondence to Jianming Wang.

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Wang, J., Tan, Y., Xu, H. et al. Effect of 2,2-azobis (2-amidinopropane) dihydrochloride oxidized casein on the microstructure and microrheology properties of emulsions. Food Sci Biotechnol 25, 1283–1290 (2016). https://doi.org/10.1007/s10068-016-0202-8

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  • DOI: https://doi.org/10.1007/s10068-016-0202-8

Keywords

  • casein
  • protein oxidation
  • emulsion stability
  • microrheology
  • microstructure