Skip to main content
Log in

Effects of raw meat and process procedure on Nε-carboxymethyllysine and Nε-carboxyethyl-lysine formation in meat products

  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

This work aimed to investigate the effects of stored raw meat and process procedures on Nε-carboxymethyl-lysine (CML) and Nε-carboxyethyl-lysine (CEL) generation in meat products. Meat products of raw pork were sterilized and pasteurized at different storage times (0-4 months) and the CML/CEL contents were determined. The results showed that the extent of lipid and protein oxidation of raw pork increased with increasing storage time. A linear correlation was found between thiobarbituric acid-reactive substances value/carbonyl content and CML/CEL in sterilized meat products, indicating that stored raw pork could promote CML/CEL formation under high temperature processing. Furthermore, mild heating temperatures seemed to favor CML formation, while high temperature could accelerate CEL generation. Therefore, formation kinetics of CML and CEL might be different for different process procedures. These results suggested that stored raw meat and processing temperature could significantly affect CML and CEL generation in meat products.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Singh R, Barden A, Mori T, Beilin L. Advanced glycation end-products: A review. Diabetologia 44: 129–146 (2001)

    Article  CAS  Google Scholar 

  2. Li M, Zeng M, He Z, Zheng Z, Qin F, Tao G, Zhang S, Chen J. Increased accumulation of protein-bound Nε-(carboxymethyl) lysiNε in tissues of healthy rats after chronic oral Nε-(carboxymethyl) lysiNε. J. Agr. Food Chem. 63: 1658–1663 (2015)

    Article  CAS  Google Scholar 

  3. Yamagishi S, Matsui T, Nakamura K. Possible link of food-derived advanced glycation end products (AGEs) to the development of diabetes. Med. Hypotheses 71: 876–878 (2008)

    Article  CAS  Google Scholar 

  4. Takeuchi M, Yamagishi SI. Possible involvement of advanced glycation endproducts (AGEs) in the pathogeNεsis of Alzheimer’s disease. Curr. Pharm. Design 14: 973–978 (2008)

    Article  CAS  Google Scholar 

  5. Sun X, Tang J, Wang J, Rasco BA, Lai K, Huang Y. Formation of advanced glycation endproducts in ground beef under pasteurisation conditions. Food Chem. 172: 802–807 (2015)

    Article  CAS  Google Scholar 

  6. Chen G, Smith JS. Determination of advanced glycation endproducts in cooked meat products. Food Chem. 168: 190–195 (2015)

    Article  CAS  Google Scholar 

  7. Poulsen MW, Hedegaard RV, Andersen JM, de Courten B, Bügel S, Nielsen J, Skibsted LH, Dragsted LO. Advanced glycation endproducts in food and their effects on health. Food Chem. Toxicol. 60: 10–37 (2013)

    Article  CAS  Google Scholar 

  8. Hull GL, Woodside JV, Ames JM, Cuskelly GJ. Nε-(carboxymethyl)lysiNε content of foods commonly consumed in a Western style diet. Food Chem. 131: 170–174 (2012)

    Article  CAS  Google Scholar 

  9. Cheng L, Jin C, Zhang Y. Investigation of Variations in the Acrylamide and Nε-(Carboxymethyl) LysiNε Contents in Cookies during Baking. J. Food Sci. 79: T1030–T1038 (2014)

    Article  CAS  Google Scholar 

  10. Goldberg T, Cai W, Peppa M, Dardaine V, Baliga BS, Uribarri J, Vlassara H. Advanced glycoxidation end products in commonly consumed foods. J. Am. Diet. Assoc. 104: 1287–1291 (2004)

    Article  CAS  Google Scholar 

  11. Lima M, Assar SH, Ames JM. Formation of Nε-(carboxymethyl) lysiNε and loss of lysiNε in casein glucose-fatty acid model systems. J. Agr. Food Chem. 58: 1954–1958 (2010)

    Article  CAS  Google Scholar 

  12. Fu Q, Li L, Li B. Formation of Nε-(Carboxymethyl) lysiNε in saccharide-lysiNε model systems by different heat treatments. Int. J. Food Eng. 8: 12578–12570 (2012)

    Article  Google Scholar 

  13. Han L, Li L, Li B, Zhao D, Li Y, Xu Z, Liu G. Glyoxal derived from triglyceride participating in diet-derived Nε-carboxymethyllysiNε formation. Food Res. Int. 51: 836–840 (2013)

    Article  CAS  Google Scholar 

  14. Martin J, Brooks J, Brooks T, Legako J, Starkey J, Jackson S, Miller M. Storage length, storage temperature, and lean formulation influence the shelf-life and stability of traditionally packaged ground beef. Meat Sci. 95: 495–502 (2013)

    Article  CAS  Google Scholar 

  15. Utrera M, Parra V, Estévez M. Protein oxidation during frozen storage and subsequent processing of different beef muscles. Meat Sci. 96: 812–820 (2014)

    Article  CAS  Google Scholar 

  16. Cheon HS, Cho WI, Yi JY, Chung MS. Optimization of sterilization conditions for retorted meatballs based on prediction of heat peNεtration. Food Sci. Biotechnol. 24: 1661–1666 (2015)

    Article  Google Scholar 

  17. Desmond EM, Kenny TA. Effect of pelvic suspension and cooking method on the processing and sensory properties of hams prepared from two pork muscles. Meat Sci. 69: 425–431 (2005)

    Article  CAS  Google Scholar 

  18. AOAC. Official Method of Analysis of AOAC Intl. 16th ed. Method 928.08. Association of Offical Analytical Chemists, Gaithersburg, MD, USA (2002)

  19. AOAC. Official Method of Analysis of AOAC Intl. 17th ed. Method 950.46. Association of Official Analytical Chemists, Gaithersburg, MD, USA (2002)

  20. Wang LL, Xiong YL. Inhibition of lipid oxidation in cooked beef patties by hydrolyzed potato protein is related to its reducing and radical scavenging ability. J. Agr. Food Chem. 53: 9186–9192 (2005)

    Article  CAS  Google Scholar 

  21. Park D, Xiong YL, Alderton AL. Concentration effects of hydroxyl radical oxidizing systems on biochemical properties of porciNε muscle myofibrillar protein. Food Chem. 101: 1239–1246 (2006)

    Article  Google Scholar 

  22. Gornall AG, Bardawill CJ, David MM. Determination of serum proteins by means of the biuret reaction. J. Biol. Chem. 171: 751–766 (1949)

    Google Scholar 

  23. Liu G, Xiong YL, Butterfield DA. Chemical, physical, and gel-forming properties of oxidized myofibrillar, whey, and soy protein isolates. J. Food Sci. 65: 811–818 (2000)

    Article  CAS  Google Scholar 

  24. Zhang G, Huang G, Xiao L, Mitchell AE. Determination of advanced glycation endproducts by LC-MS/MS in raw and roasted almonds (Prunus dulcis). J. Agr. Food Chem. 59: 12037–12046 (2011)

    Article  CAS  Google Scholar 

  25. Kong B, Zhang H, Xiong YL. Antioxidant activity of spice extracts in a liposome system and in cooked pork patties and the possible mode of action. Meat Sci. 85: 772–778 (2010)

    Article  CAS  Google Scholar 

  26. Brewer MS, Ikins W, Harbers C. TBA values, sensory characteristics, and volatiles in ground pork during long-term frozen storage: Effects of packaging. J. Food Sci. 57: 558–563 (1992)

    Article  CAS  Google Scholar 

  27. Vieira C, Diaz MY, Martínez B, García-Cachán MD. Effect of frozen storage conditions (temperature and length of storage) on microbial and sensory quality of rustic crossbred beef at different stages of aging. Meat Sci. 83: 398–404 (2009)

    Article  CAS  Google Scholar 

  28. Stadtman ER. Protein oxidation and aging. Free Radical Res. 40: 1250–1258 (2006)

    Article  CAS  Google Scholar 

  29. Stadtman ER, Levine RL. Free radical-mediated oxidation of free amino acids and amino acid residues in proteins. Amino Acids 25: 207–218 (2003)

    Article  CAS  Google Scholar 

  30. Estévez M. Protein carbonyls in meat systems: A review. Meat Sci. 89: 259–279 (2011)

    Article  Google Scholar 

  31. Srey C, Hull GL, Connolly L, Elliott CT, del Castillo MD, Ames JM. Effect of inhibitor compounds on Nε-(Carboxymethyl)lysiNε (CML) and Nε-(Carboxyethyl)lysiNε (CEL) formation in model foods. J. Agr. Food Chem. 58: 12036–12041 (2010)

    Article  CAS  Google Scholar 

  32. Arribas-Lorenzo G, Morales FJ. Analysis, distribution, and dietary exposure of glyoxal and methylglyoxal in cookies and their relationship with other heatinduced contaminants. J. Agr. Food Chem. 58: 2966–2972 (2010)

    Article  CAS  Google Scholar 

  33. Ahmed N, Mirshekar-Syahkal B, Kennish L, Karachalias N, Babaei-Jadidi R, Thornalley PJ. Assay of advanced glycation endproducts in selected beverages and food by liquid chromatography with tandem mass spectrometric detection. Mol. Nutr. Food Res. 49: 691–699 (2005)

    Article  CAS  Google Scholar 

  34. Li CQ, Xiong YL, Chen J. Oxidation-induced unfolding facilitates myosin crosslinking in myofibrillar protein by microbial transglutaminase. J. Agr. Food Chem. 60: 8820–8827 (2012)

    Google Scholar 

  35. Fu MX, Requena JR, Jenkins AJ, Lyons TJ, Baynes JW, Thorpe SR. The advanced glycation end product, Nε-(carboxymethyl)lysiNε, is a product of both lipid peroxidation and glycoxidation reactions. J. Biol. Chem. 271: 9982–9986 (1996)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jie Chen.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yu, L., Gao, C., Zeng, M. et al. Effects of raw meat and process procedure on Nε-carboxymethyllysine and Nε-carboxyethyl-lysine formation in meat products. Food Sci Biotechnol 25, 1163–1168 (2016). https://doi.org/10.1007/s10068-016-0185-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-016-0185-5

Keywords

Navigation