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Chymotrypsin isolation from jumbo squid (Dosidicus gigas) hepatopancreas: Partial characterization and effect on muscle collagen

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Abstract

Chymotrypsin was purified from jumbo squid hepatopancreas (HP) with 2.4-fold and yield 1.9%, and characterized with a molecular weight of 31 kDa, as estimated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE). Chymotrypsin effect over collagen extracted from the mantle, fins and arms of the jumbo squid was evaluated. The enzyme exhibited the maximum activity at pH 7 and 65°C using Suc-Ala-Ala-Pro-Phe-p-nitroanilide (SAAPNA) as a substrate and it was identified using the specific inhibitors N-tosyl-L-phenylalaninechloromethyl ketone (TPCK) and phenyl methyl sulfonyl fluoride (PMSF), showing residual activities of 6% and 0%, respectively. Furthermore, high activity was observed in the pH range of 4.0 to 8.0. Purified enzyme showed a moderate in vitro activity using muscle collagen as a substrate. Although further research is needed, the results suggest that the enzyme has a potential application where acidic or slightly alkaline conditions are needed.

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Authors and Affiliations

  1. Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Blvd. Luis Encinas y Rosales s/n. Apdo. Postal 1658, C.P. 83000 Col. Centro, Hermosillo, Sonora, México

    Enrique Marquez-Rios, Ana Gloria Villalba-Villalba, Josafat Marina Ezquerra-Brauer, Betzabe Ebenhezer Lopez-Corona & Wilfrido Torres-Arreola

  2. Ingeniería Ambiental. Universidad Estatal de Sonora, Unidad Académica Hermosillo. Ley Federal del Trabajo S/N, Hermosillo, Sonora, México

    Octavio Cota-Arriola & Ana Gloria Villalba-Villalba

  3. Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Blvd. Luis Encinas y Rosales s/n. Apdo. Postal 1658, C.P. 83000 Col. Centro, Hermosillo, Sonora, México

    Victor Manuel Ocaño-Higuera

Authors
  1. Enrique Marquez-Rios
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  2. Octavio Cota-Arriola
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  3. Ana Gloria Villalba-Villalba
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  4. Josafat Marina Ezquerra-Brauer
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  5. Victor Manuel Ocaño-Higuera
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  6. Betzabe Ebenhezer Lopez-Corona
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  7. Wilfrido Torres-Arreola
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Correspondence to Wilfrido Torres-Arreola.

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Marquez-Rios, E., Cota-Arriola, O., Villalba-Villalba, A.G. et al. Chymotrypsin isolation from jumbo squid (Dosidicus gigas) hepatopancreas: Partial characterization and effect on muscle collagen. Food Sci Biotechnol 25, 1011–1016 (2016). https://doi.org/10.1007/s10068-016-0163-y

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  • DOI: https://doi.org/10.1007/s10068-016-0163-y

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