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Method validation of analytical method for 12 flavonol glycosides in foods using ultra high-performance liquid chromatography coupled with photodiode array detection

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Abstract

An analytical method for the simultaneous determination of 12 flavonol glycosides in buckwheat, black tea, and wild parsley using ultra high-performance liquid chromatography (UHPLC) coupled with a simple liquid extraction method using dimethyl sulfoxide (DMSO) was validated in precision, accuracy, and linearity. The UHPLC separation of target compounds was performed on a C18 column using a photodiode array (PDA) detector and the wavelength was fixed at 350 nm. The recovery values for flavonol glycosides ranged from 85.44 to 108.79%. The limits of detection and limits of quantification were less than 0.32 mg/kg and less than 0.97 mg/kg, respectively. The intraday and interday precisions were less than 13.69% for all the test samples. This method coupled with UHPLCPDA detection could be expected to provide more convenient sample preparation than conventional methods in the tested foods.

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Authors and Affiliations

  1. Food Standard Research Center, Korea Food Research Institute, Seongnam, Gyeonggi, 13539, Korea

    Jong-Chan Kim & You-Shin Shim

  2. Department of Food Biotechnology, University of Science & Technology, Daejeon, 34113, Korea

    You-Shin Shim

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  1. Jong-Chan Kim
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  2. You-Shin Shim
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Correspondence to You-Shin Shim.

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Kim, JC., Shim, YS. Method validation of analytical method for 12 flavonol glycosides in foods using ultra high-performance liquid chromatography coupled with photodiode array detection. Food Sci Biotechnol 25, 659–664 (2016). https://doi.org/10.1007/s10068-016-0116-5

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  • DOI: https://doi.org/10.1007/s10068-016-0116-5

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