Porcine hemoglobin promotes lipid excretion to feces more strongly than globin protein in rats
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In an effort to clarify whether the lipid-lowering effect of hemoglobin is due to globin protein or heme, this study investigated the effects of dietary porcine hemoglobin (PH) and porcine globin (PG) on lipids contents of serum, liver, and feces in rats. Five-week-old male Wistar rats were divided into 3 dietary groups of 7 rats each, with one group receiving a control diet and the other groups receiving diets containing 1.25% (w/w) PH or 1.18% (w/w) PG for 4 weeks. The PH diet decreased triacylglycerol content in serum and cholesterol in serum and liver, whereas the PG diet reduced triacylglycerol content in serum and cholesterol in liver. Fecal lipid excretion in the PH group was significantly higher than in the PG group. Therefore, PH affected lipid excretion to feces due to globin protein as well as heme.
Keywordsporcine hemoglobin globin heme lipid excretion cholesterol
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- 1.Nowak B, von Mueffling T. Porcine blood cell concentrates for food products: Hygiene, composition, and preservation. J. Food Protect. 69: 2183–2192 (2006)Google Scholar
- 13.Reeves PG, Nielsen FH, Fahey GC Jr. AIN-93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J. Nutr. 123: 1939–1951 (1993)Google Scholar
- 16.Van De Kamer JH, Bokkel Huinink HT, Weyers HA. Rapid method for the determination of fat in feces. J. Biol. Chem. 177: 347–355 (1949)Google Scholar
- 19.Markwell MA, McGroarty EJ, Bieber LL, Tolbert NE. The subcellular distribution of carnitine acyltransferases in mammalian liver and kidney. A new peroxisomal enzyme. J. Biol. Chem. 248: 3426–3432 (1973)Google Scholar
- 22.Hsu RY, Lardy HA. Malic enzyme. Vol. 13, pp.230–235, In: Methods Enzymol. Lowebsein LM (ed). Academic Press, New York, NY, USA (1969)Google Scholar
- 24.Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275 (1951)Google Scholar
- 26.Uzel C, Conrad ME. Absorption of heme iron. Semin. Hematol. 35: 27–34 (1998)Google Scholar
- 28.Uemura K, Hosomi R, Fukunaga K, Nishiyama T, Yoshida M. Effect of difference in chemical species of dietary iron on iron status in rats. Trace Nutr. Res. 27: 13–16 (2010) (in Japanese)Google Scholar
- 29.Moriyama T, Kishimoto K, Nagai K, Urade R, Ogawa T, Utsumi S, Maruyama N, Maebuchi M. Soybean beta-conglycinin diet suppresses serum triglyceride levels in normal and genetically obese mice by induction of beta-oxidation, downregulation of fatty acid synthase, and inhibition of triglyceride absorption. Biosci. Biotech. Bioch. 68: 352–359 (2004)CrossRefGoogle Scholar
- 32.Morita T, Oh-hashi A, Takei K, Ikai M, Kasaoka S, Kiriyama S. Cholesterollowering effects of soybean, potato and rice proteins depend on their low methionine contents in rats fed a cholesterol-free purified diet. J. Nutr. 127: 470–477 (1997)Google Scholar
- 33.Nagata Y, Ishiwaki N, Sugano M. Studies on the mechanism of antihypercholesterolemic action of soy protein and soy protein-type amino acid mixtures in relation to the casein counterparts in rats. J. Nutr. 112: 1614–1625 (1982)Google Scholar