Skip to main content
SpringerLink
Log in

Insect tea extract attenuates CCl4-induced hepatic damage through its antioxidant capacities in ICR mice

  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

The Insect tea extract (ITE) contained many polyphenols, the aim of the present study was to determine the preventive effects of ITE on CCl4-induced hepatic damage in mice. ITE treated mice could reduce hepatic injury compared to the control mice. The 200 mg/kg ITE increased TC, ALB, SOD, CAT, GSH-Px serum levels, and decreased ALT, AST, ALP, TG, BUN, NO, MDA levels compared to the control group. By histological observation, ITE reduced injury to hepatic cells, and these effects were close to that seen with the drug silymarin. The antioxidant related mRNA and protein expressions of Mn SOD, Gu/Zn SOD, CAT, and GSH-Px increased with ITE treatment in hepatic damage mice. ITE treated mice also showed higher IκB-α mRNA and protein expression, and lower NF-κB-p65, iNOS, COX-2 expressions than those of control mice. These results proved ITE has a prophylactic effect in protecting against hepatic injury through the antioxidant capacities.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Qian Y, Li GJ, Wang R, Zhou YL, Sun P, Zhao X. In vitro anticancer effects of insect tea in TCA8113 cells. J. Canc. Res. Ther. 10: 1045–1051 (2014)

    Article  Google Scholar 

  2. Zhao X, Wang R, Qian Y, Li G, Zhou Y, Sun P. In vivo preventive effects of insect tea on buccal mucosa cancer in ICR mice. J. Canc. Res. Ther. 10: 651–657 (2014)

    Google Scholar 

  3. Zhou YL, Wang R, Feng X, Zhao X. Preventive effect of insect tea against reserpine-induced gastric ulcers in mice. Exp. Ther. Med. 8: 1318–1324 (2014)

    Google Scholar 

  4. Cheng X, Xie CL, Wang J. Changes of serum nitric oxide level after acute hepatic injury in mice and the effect of yugan capsule on it. Chin. J. Integr. Tradit. West Med. 26: 58–60 (2006)

    Google Scholar 

  5. Sharifi R, Pasalar P, Kamalinejad M, Dehpour AR, Tavangar SM, Paknejad M, Mehrabani Natanzi M, Nourbakhsh M, Ahmadi Ashtiani HR, Akbari M, Rastegar H. The effect of silymarin (Silybum marianum) on human skin fibroblasts in an in vitro wound healing model. Pharm. Biol. 51: 298–303 (2013)

    Article  CAS  Google Scholar 

  6. Gound SS, Thakare VN, Khan S, Wadekar RR, Naik SR. Ameliorative effects of Tricholepis glaberrima in experimentally induced hepatic damage in rats: Modulation of cytokines functions. J. Ethnopharmacol. 160: 164–172 (2015)

    Article  Google Scholar 

  7. Zhao X, Wang Q. Antioxidant, antimutagenic and in vitro anticancer effects of crude polyphenols in Insect tea made from Kuding tea leaves. Mod. Food Sci. Technol. 31: 24–28 (2015)

    Google Scholar 

  8. Zhou YL, Feng X, Zhu K, Zhao X. Apoptosis inducing effects of crude polyphenols of Insect tea made by Kuding tea leavses in HepG2 human hepatoma cells. Sci. Technol. Food Ind. 9: 339–342 (2015)

    Google Scholar 

  9. Fu S, Wang F, Cao Y, Huang Q, Xiao J, Yang C, Popescu LM. Telocytes in human liver fibrosis. J. Cell Mol. Med. 19: 676–683 (2015)

    Article  CAS  Google Scholar 

  10. Xu GM, Peng XJ, Wang CJ, Wen LZ, Liu WL, Jiang XM. Extraction of polyphenols in aglossa dimidiata with ions Al3+ and Zn2+ sedimentation. J. TCM Univ. Hunan 2: 46–46 (2007)

    Google Scholar 

  11. Zhao X, Kim SY, Park KY. Bamboo salt has in vitro anticancer activity in HCT-116 cells and exerts anti-metastatic effects in vivo. J. Med. Food 16: 9–19 (2013)

    Article  Google Scholar 

  12. Zhao X, Song JL, Kil JH, Park KY. Bamboo salt attenuates CCl4-induced hepatic damage in Sprague-Dawley rats. Nutr. Res. Pract. 7: 273–280 (2013)

    Article  CAS  Google Scholar 

  13. Guo SY, Xu WX, Wen LZ, Huang YM, Wang F. The nutrient analysis and evaluation of Sanye insect-fermented tea. Chinese Bull. Entimol. 45: 128–132 (2008)

    CAS  Google Scholar 

  14. Wang R, Sun P, Zhao X. Protective effects of crude polysaccharide in Insect tea against carbon tetrachloride-induced hepatic damage in mice. Mod. Food Sci. Technol. 31:6–11 (2015)

    Google Scholar 

  15. Lu B, Xu Y, Xu L, Cong X, Yin L, Li H, Peng J. Mechanism investigation of dioscin against CCl4-induced acute liver damage in mice. Environ. Toxicol. Phar. 34: 127–135 (2012)

    Article  CAS  Google Scholar 

  16. Xin X, Yang W, Yasen M, Zhao H, Aisa H. The mechanism of hepatoprotective effect of sesquiterpene rich fraction from Cichorum glandulosum Boiss. et Huet on immune reaction-induced liver injury in mice. J. Ethnopharmacol. 155: 1068–1075 (2014)

    CAS  Google Scholar 

  17. Wang T, Zhou ZX, Sun LX, Li X, Xu ZM, Chen M, Zhao GL, Jiang ZZ, Zhang LY. Resveratrol effectively attenuates a-naphthyl-isothiocyanate-induced acute cholestasis and liver injury through choleretic and anti-inflammatory mechanisms. Acta Pharmacol. Sin. 35: 1527–1536 (2014)

    Article  Google Scholar 

  18. Wang Z, Su B, Fan S, Fei H, Zhao W. P rotective effect of oligomeric proanthocyanidins against alcohol-induced liver steatosis and injury in mice. Biochem. Bioph. Res. Co. 458: 757–762 (2015)

    Article  CAS  Google Scholar 

  19. Patankar JV, Chandak PG, Obrowsky S, Pfeifer T, Diwoky C, Uellen A, Sattler W, Stollberger R, Hoefler G, Heinemann A, Battle M, Duncan S, Kratky D, Levak-Frank S. Loss of intestinal GATA4 prevents diet-induced obesity and promotes insulin sensitivity in mice. Am. J. Physiol. Endocrinol. Metab. 300: 478–488 (2011)

    Article  Google Scholar 

  20. Takahashi H, Shigefuku R, Yoshida Y, Ikeda H, Matsunaga K, Matsumoto N, Okuse C, Sase S, Itoh F, Suzuki M. Correlation between hepatic blood flow and liver function in alcoholic liver cirrhosis. World J. Gastroenterol. 20: 17065–17074 (2014)

    Article  Google Scholar 

  21. Araki H, Ueda H, Fujimoto S. Immunocytochemical localization of alphafetoprotein in the developing and carbon tetrachloride-treated rat liver. Acta Anat. 143: 169–177 (1992)

    Article  CAS  Google Scholar 

  22. Dabiri H, Azimi Rad M, Tavafzadeh R, Taheri E, Safakar S, Nazemalhosseini Mojarad E, Farzaneh N, Zali MR. Bacteriologic study of cirrhotic patients with non-neutrocytic ascites. Gastroenterol. Hepatol. Bed Bench. 7: 224–229 (2014)

    Google Scholar 

  23. Lee KJ, Jeong HG. Protective effect of Platycodi radix on carbon tetrachlorideinduced hepatotoxicity. Food Chem. Toxicol. 40: 517–525 (2002)

    Article  CAS  Google Scholar 

  24. Ioannidis I, de Groot H. Cytotoxicity of nitric oxide in Fu5 rat hepatoma cells: Evidence for co-operative action with hydrogen peroxide. Biochem. J. 296: 341–345 (1993)

    Article  CAS  Google Scholar 

  25. Thakore KN, Mehendale HM. Role of hepatocellular regeneration in CCl4 autoprotection. Toxicol. Pathol. 19: 47–58 (1991)

    CAS  Google Scholar 

  26. Krithika R, Verma RJ, Shrivastav PS, Suguna L. Phyllanthin of standardized phyllanthus amarus extract attenuates liver oxidative stress in mice and exerts cytoprotective activity on human hepatoma cell line. J. Clin. Exp. Hepatol. 1: 57–67 (2011)

    Article  CAS  Google Scholar 

  27. Remmer H, Hintze T, Frank H, Müh-Zange M. Cytochrome P-450 oxidation of alkanes originating as scission products during lipid peroxidation. Xenobiotica 14: 207–219 (1984)

    Article  CAS  Google Scholar 

  28. Wang B, Bai M, Bai Y, Li Q. Liver injury following renal ischemia reperfusion in rats. Transplant. P. 42: 3422–3426 (2010)

    Article  CAS  Google Scholar 

  29. Li W, Qu XN, Han Y, Zheng SW, Wang J, Wang YP. Ameliorative effects of 5-hydroxymethyl-2-furfural (5-HMF) from Schisandra chinensis on alcoholic liver oxidative injury in mice. Int. J. Mol. Sci. 16: 2446–2457 (2015)

    Article  CAS  Google Scholar 

  30. Semenov DE, Zhukova NA, Ivanova EP, Sorokina IV, Baiev DS, Nepomnyashchikh GI, Tolstikova TG, Biryukova MS. Hepatoprotective properties of betulonic acid amide and heptral in toxic liver injury induced by carbon tetrachloride in combination with ethanol. B. Exp. Biol. Med. 158: 336–341 (2015)

    Article  CAS  Google Scholar 

  31. Curry-McCoy TV, Osna NA, Nanji AA, Donohue TM. Chronic ethanol consumption results in atypical liver injury in copper/zinc superoxide dismutase deficient mice. Alcohol. Clin. Exp. Res. 34: 251–261 (2010)

    Article  CAS  Google Scholar 

  32. Pan H, Chen J, Shen K, Wang X, Wang P, Fu G, Meng H, Wang Y, Jin B. Mitochondrial modulation by epigallocatechin 3-gallate ameliorates cisplatin induced renal injury through decreasing oxidative/nitrative stress, inflammation and NF-kB in mice. PLoS ONE 10: e0124775 (2015)

    Article  Google Scholar 

  33. Kim HR, Shin DY, Chung KH. The role of NF-kB signaling pathway in polyhexamethylene guanidine phosphate induced inflammatory response in mouse macrophage RAW264.7 cells. Toxicol. Lett. 233: 148–155 (2015)

    Article  CAS  Google Scholar 

  34. Ma XY, Wang H, Ding B, Zhong H, Ghosh S, Lengyel P. The interferon-inducible p202a protein modulates NF-kappaB activity by inhibiting the binding to DNA of p50/p65 heterodimers and p65 homodimers while enhancing the binding of p50 homodimers. J. Biol. Chem. 278: 23008–23019 (2003)

    Article  CAS  Google Scholar 

  35. Murley JS, Kataoka Y, Hallahan DE, Roberts JC, Grdina DJ. Activation of NFkB and MnSOD gene expression by free radical scavengers in human microvascular endothelial cells. Free Radical Bio. Med. 30: 1426–1439 (2001)

    Article  CAS  Google Scholar 

  36. Kaur J, Shalini S, Bansal MP. Influence of vitamin E on alcohol-induced changes in antioxidant defenses in mice liver. Toxicol. Mech. Method. 20: 82–89 (2010)

    Article  CAS  Google Scholar 

  37. Xu D, Hu L, Su C, Xia X, Zhang P, Fu J, Wang W, Xu D, Du H, Hu Q, Song E, Song Y. Tetrachloro-p-benzoquinone induces hepatic oxidative damage and inflammatory response, but not apoptosis in mouse: The prevention of curcumin. Toxicol. Appl. Pharm. 280: 305–313 (2014)

    Article  CAS  Google Scholar 

  38. Das P, De T, Chakraborti T. Leishmania donovani secretory serine protease alters macrophage inflammatory response via COX-2 mediated PGE-2 production. Indian J. Biochem. Biophys. 51: 542–551 (2014)

    CAS  Google Scholar 

  39. Lin X, Liu X, Huang Q, Zhang S, Zheng L, Wei L, He M, Jiao Y, Huang J, Fu S, Chen Z, Li Y, Zhuo L, Huang R. Hepatoprotective effects of the polysaccharide isolated from Tarphochlamys affinis (Acanthaceae) against CCl4-induced hepatic injury. Biol. Pharm. Bull. 35: 1574–1580 (2012)

    Article  CAS  Google Scholar 

  40. Chen TH, Liao FT, Yang YC, Wang JJ. Inhibition of inducible nitric oxide synthesis ameliorates liver ischemia and reperfusion injury induced transient increase in arterial stiffness. Transplant. P. 46: 1112–1116 (2014)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Nephrology, The Ninth People’s Hospital of Chongqing, Chongqing, 400700, China

    Yuanjiang Liao

  2. Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, 400067, China

    Xiaochuan Zou, Cun Wang & Xin Zhao

  3. Chongqing Collaborative Innovation Center of Functional Food, Chongqing University of Education, Chongqing, 400067, China

    Xiaochuan Zou, Cun Wang & Xin Zhao

  4. Chongqing Engineering Technology Research Center for Functional Food, Chongqing University of Education, Chongqing, 400067, China

    Xiaochuan Zou, Cun Wang & Xin Zhao

  5. Chongqing Engineering Laboratory of Functional Food, Chongqing University of Education, Chongqing, 400067, China

    Xiaochuan Zou, Cun Wang & Xin Zhao

Authors
  1. Yuanjiang Liao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiaochuan Zou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xin Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xin Zhao.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liao, Y., Zou, X., Wang, C. et al. Insect tea extract attenuates CCl4-induced hepatic damage through its antioxidant capacities in ICR mice. Food Sci Biotechnol 25, 581–587 (2016). https://doi.org/10.1007/s10068-016-0081-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-016-0081-z

Keywords

Search

Navigation