Abstract
Weissella confusa AJ53 isolated from fermented Uttapam batter supplemented with Piper betle L. leaves produced an exopolysaccharide (EPS) from sucrose. The optimum culture conditions were determined for maximum production. EPS AJ53 was characterized using spectral analysis as an unbranched linear (α-1→6 linked) dextran, homopolymer of D-glucose. Melting and degradation temperatures of EPS AJ53 were 103.84 and 265°C, respectively. EPS AJ53 exhibited antioxidant activities for scavenging of hydroxyl, DPPH, and nitric oxide radicals, and metal ion chelating and poor reducing abilities. A fat binding ability, oil emulsifying activity, and syneresis-prevention activity due to a water holding capacity were demonstrated. Application as an adjunct to provide value addition to food products is indicated. A potent flocculating ability enables EPS AJ53 to be used for water treatment and cryoprotectant applications as an alternative cryoprotective agent. The yield and properties of EPS AJ53 indicate multiple uses.
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Dubey, A.K., Jeevaratnam, K. Structural characterization and functional evaluation of an exopolysaccharide produced by Weissella confusa AJ53, an isolate from fermented Uttapam batter supplemented with Piper betle L. leaves. Food Sci Biotechnol 24, 2117–2124 (2015). https://doi.org/10.1007/s10068-015-0281-y
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DOI: https://doi.org/10.1007/s10068-015-0281-y