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Comparative study of fecal microbiota in patients with type II diabetes after consumption of apple juice for 4 weeks

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Abstract

The effect of cloudy apple juice on fecal microbiota of type 2 diabetics was studied. Five volunteers consumed apple juice while 5 control volunteers received an isocaloric control beverage daily for 4 weeks. DGGE profile analysis showed high diversity between volunteers that did not change over the intervention period using primers for Firmicutes, Bacteroidetes, bifidobacteria, enterococci, and enterobacteria. An exception was observed using lactobacilli primers, perhaps as the result of the dietary influence. Consumption of apple juice was not correlated with changes in DGGE profiles. Quantitative PCR was used to investigate the effect of apple juice on bacterial counts in different subgroups. Apple juice did not lead to significantly (p>0.05) different numbers of total bacteria, enterobacteria, bifidobacteria, lactobacilli, or Bacteroidetes, but caused a significant (p<0.05) decrease in numbers of enterococci, and a smaller but also significant decrease in numbers of Firmicutes, when comparing before and after intervention with apple juice.

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Authors and Affiliations

  1. Department of Microbiology and Biotechnology, Max Rubner-Institut Federal Research Institute for Nutrition and Food, Kiel, Germany

    Gyu-Sung Cho & Charles M. A. P. Franz

  2. Department of Safety and Quality of Fruit and Vegetables, Karlsruhe, Germany

    Anja König, Alexander Hanak & Melanie Huch

  3. Department of Physiology and Biochemistry of Nutrition, Karlsruhe, Germany

    Stephanie Seifert, Alexander Roth, Achim Bub & Bernhard Watzl

Authors
  1. Gyu-Sung Cho
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  2. Anja König
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  3. Stephanie Seifert
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  4. Alexander Hanak
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  7. Achim Bub
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  9. Charles M. A. P. Franz
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Correspondence to Gyu-Sung Cho.

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Cho, GS., König, A., Seifert, S. et al. Comparative study of fecal microbiota in patients with type II diabetes after consumption of apple juice for 4 weeks. Food Sci Biotechnol 24, 2083–2094 (2015). https://doi.org/10.1007/s10068-015-0277-7

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  • DOI: https://doi.org/10.1007/s10068-015-0277-7

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