Food Science and Biotechnology

, Volume 24, Issue 5, pp 1887–1892 | Cite as

Effect of pomegranate peel polyphenols on human prostate cancer PC-3 cells in vivo

  • Gui-Zhi Ma
  • Chun-Mei Wang
  • Li Li
  • Nan Ding
  • Xiao-Li GaoEmail author


Punica granatum possesses strong antitumor properties. In this study, a nude mouse model with a subcutaneous xenograft of human prostate cancer cells (PC-3) was established to observe the in vivo antiproliferative and apoptotic effects of pomegranate peel polyphenols (PP). Levels of the cytokines tumor necrosis factor α (TNF-α) and vascular endothelial growth factor (VEGF) were detected by enzyme-linked immunosorbent assay. Quantitative analysis of ellagic acid, gallic acid, and punicalagin in active fraction was conducted using reverse phase high-performance liquid chromatography with a photo-diode array (HPLC/PDA). PP decreased tumor volume and weight in tumor-bearing nude mice, and significantly increased the rate of apoptosis (p<0.05). In addition, PP increased TNF-α and decreased VEGF in the serum (p<0.05). Ellagic acid (201.3±3.544 mg/g), gallic acid (8.917±0.274 mg/g), and punicalagin (407.0±12.05 mg/g) were the main effectors of the anti-tumor activity.


pomegranate peel polyphenols prostate cancer TNF-α VEGF HPLC/PDA 


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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Gui-Zhi Ma
    • 1
  • Chun-Mei Wang
    • 2
  • Li Li
    • 3
  • Nan Ding
    • 2
  • Xiao-Li Gao
    • 1
    Email author
  1. 1.College of PharmacyXinjiang Medical UniversityUrumqiChina
  2. 2.The First Teaching Hospital of Xingjiang Medical UniversityUrumqiChina
  3. 3.Beijing Key Laboratory of Plant Resources Research and DevelopmentBeijing Technology and Business UniversityBeijingChina

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