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Examination of the effects of temperature and pressure on lipoxygenase activities in pork using response surface methodology

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Abstract

To investigate the effects of pressure and temperature on lipoxygenase (LOX) in meat, changes in LOX activities in minced pork and in crude enzyme extracts under different conditions of pressure (131.82–468.18 MPa), temperature (23.18–56.82°C), and duration (6.59–23.41 min) were evaluated using response surface methodology. LOX activities in extracts exhibited a decrease with an increasing treatment temperature, pressure, or duration. Each of these factors alone had a significant (p<0.05) effect on LOX activities. Activation of LOX in most minced pork treated at less than 400 MPa was observed. Treatment pressure and temperature, and interactions between pressure and temperature and between duration and pressure, significantly (p<0.05) affected LOX activities. LOX showed higher stability in muscles than in extracts during combined pressure-heat treatments. The most important factor affecting LOX was treatment temperature for extracts and treatment pressure for muscles.

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References

  1. Fu X, Xu S, Wang Z. Kinetics of lipid oxidation and off-odor formation in silver carp mince: The effect of lipoxygenase and hemoglobin. Food Res. Int. 42: 85–90 (2009)

    Article  CAS  Google Scholar 

  2. Gata JL, Pinto MC, Macías P. Lipoxygenase activity in pig muscle: Purification and partial characterization. J. Agr. Food Chem. 44: 2573–2577 (1996)

    Article  CAS  Google Scholar 

  3. German JB, Creveling RK. Identification and characterization of a 15-lipoxygenase from fish gills. J. Agr. Food Chem. 38: 2144–2147 (1990)

    Article  CAS  Google Scholar 

  4. Grossman S, Bergman M, Sklan D. Lipoxygenase in chicken muscle. J. Agr. Food Chem. 36: 1268–1270 (1988)

    Article  CAS  Google Scholar 

  5. Hsieh RJ, German JB, Kinsella JE. Lipoxygenase in fish tissue: Some properties of the 12-lipoxygenase from trout gill. J. Agr. Food Chem. 36: 680–685 (1988)

    Article  CAS  Google Scholar 

  6. Hsu HH, Pan BS. Effects of protector and hydroxyapatite partial purification on stability of lipoxygenase from gray mullet gill. J. Agr. Food Chem. 44: 741–745 (1996)

    Article  CAS  Google Scholar 

  7. Kuo J, Pan BS, Zhang H, German JB. Identification of 12-lipoxygenase in the hemolymph of tiger shrimp (Penaeus japonicus Bate). J. Agr. Food Chem. 42: 1620–1623 (1994)

    Article  CAS  Google Scholar 

  8. Saeed S, Howell NK. 12-Lipoxygenase activity in the muscle tissue of Atlantic mackerel (Scomber scombrus) and its prevention by antioxidants. J. Sci. Food Agr. 81: 745–750 (2001)

    Article  CAS  Google Scholar 

  9. Martn L, Córdoba JJ, Ventanas J, Antequera T. Changes in intramuscular lipids during ripening of Iberian dry-cured ham. Meat Sci. 51: 129–134 (1999)

    Article  Google Scholar 

  10. Toldrá F. Proteolysis and lipolysis in flavour development of drycured meat products. Meat Sci. 49: S101–S110 (1998)

    Article  Google Scholar 

  11. Yang H, Ma C, Qiao F, Song Y, Du M. Lipolysis in intramuscular lipids during processing of traditional Xuanwei ham. Meat Sci. 71: 670–675 (2005)

    Article  CAS  Google Scholar 

  12. Cheftel JC, Culioli J. Effects of high pressure on meat: A review. Meat Sci. 46: 211–236 (1997)

    Article  CAS  Google Scholar 

  13. Orlien V, Hansen E, Skibsted LH. Lipid oxidation in high-pressure processed chicken breast muscle during chill storage: Critical working pressure in relation to oxidation mechanism. Eur. Food Res. Technol. 211: 99–104 (2000)

    Article  CAS  Google Scholar 

  14. Torres JA, Velazquez G. Commercial opportunities and research challenges in the high pressure processing of foods. J. Food Eng. 67: 95–112 (2005)

    Article  Google Scholar 

  15. Seyderhelm I, Boguslawski S, Michaelis G, Knorr D. Pressure induced inactivation of selected food enzymes. J. Food Sci. 61: 308–310 (1996)

    Article  CAS  Google Scholar 

  16. Indrawati, Van Loey AM, Ludikhuyze LR, Hendrickx ME. Soybean lipoxygenase inactivation by pressure at subzero and elevated temperatures. J. Agr. Food Chem. 47: 2468–2474 (1999)

    Article  CAS  Google Scholar 

  17. Ludikhuyze L, Indrawati, Van den Broeck I, Weemaes C, Hendrickx M. Effect of combined pressure and temperature on soybean lipoxygenase. 1. Influence of extrinsic and intrinsic factors on isobaric-isothermal inactivation kinetics. J. Agr. Food Chem. 46: 4074–4080 (1998)

    CAS  Google Scholar 

  18. Heinisch O, Kowalski E, Goossens K, Frank J, Heremans K, Ludwig H, Tauscher B. Pressure effects on the stability of lipoxygenase: Fourier transform-infrared spectroscopy (FT-IR) and enzyme activity studies. Z. Lebensm Unters For. 201: 562–565 (1995)

    Article  CAS  Google Scholar 

  19. Tedjo W, Eshtiaghi MN, Knorr D. Impact of supercritical carbon dioxide and high pressure on lipoxygenase and peroxidase activity. J. Food Sci. 65: 1284–1287 (2000)

    Article  CAS  Google Scholar 

  20. Anese M, Sovrano S. Kinetics of thermal inactivation of tomato lipoxygenase. Food Chem. 95: 131–137 (2006)

    Article  CAS  Google Scholar 

  21. Indrawati, Van Loey AM, Ludikhuyze LR, Hendrickx ME. Pressure-temperature inactivation of lipoxygenase in green peas (Pisum sativum): A kinetic study. J. Food Sci. 66: 686–693 (2001)

    Article  CAS  Google Scholar 

  22. Wang R, Zhou X, Chen Z. High pressure inactivation of lipoxygenase in soy milk and crude soybean extract. Food Chem. 106: 603–611 (2008)

    Article  CAS  Google Scholar 

  23. Indrawati I, Ludikhuyze LR, Van Loey AM, Hendrickx ME. Lipoxygenase inactivation in green beans (Phaseolus vulgaris L.) due to high pressure treatment at subzero and elevated temperatures. J. Agr. Food Chem. 48: 1850–1859 (2000)

    Article  CAS  Google Scholar 

  24. Jin G, Zhang J, Yu X, Lei Y, Wang J. Crude lipoxygenase from pig muscle: Partial characterization and interactions of temperature, NaCl and pH on its activity. Meat Sci. 87: 257–263 (2011)

    Article  CAS  Google Scholar 

  25. Homma N, Ikeuchi Y, Suzuki A. Effect of high pressure treatment on proteolytic system in meat. Progr. Biotechnol. 13: 327–330 (1996)

    Article  CAS  Google Scholar 

  26. Lakshmanan R, Patterson MF, Piggott JR. Effects of high-pressure processing on proteolytic enzymes and proteins in cold-smoked salmon during refrigerated storage. Food Chem. 90: 541–548 (2005)

    Article  CAS  Google Scholar 

  27. Terefe NS, Yang YH, Knoerzer K, Buckow R, Versteeg C. High pressure and thermal inactivation kinetics of polyphenol oxidase and peroxidase in strawberry puree. Innov. Food Sci. Emerg. 11: 52–60 (2010)

    Article  CAS  Google Scholar 

  28. Jung S, Ghoul M, de Lamballerie-Anton M. Changes in lysosomal enzyme activities and shear values of high pressure treated meat during ageing. Meat Sci. 56: 239–246 (2000)

    Article  CAS  Google Scholar 

  29. Rodrigo D, Jolie R, Van Loey A, Hendrickx M. Thermal and high pressure stability of tomato lipoxygenase and hydroperoxide lyase. J. Food Eng. 79: 423–429 (2007)

    Article  CAS  Google Scholar 

  30. Bayindirli A, Alpas H, Bozoglu F, Hizal M. Efficiency of high pressure treatment on inactivation of pathogenic microorganisms and enzymes in apple, orange, apricot and sour cherry juices. Food Control 17: 52–58 (2006)

    Article  CAS  Google Scholar 

  31. He Z, Huang Y, Li H, Qin G, Wang T, Yang J. Effect of highpressure treatment on the fatty acid composition of intramuscular lipid in pork. Meat Sci. 90: 170–175 (2012)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China

    Yechuan Huang, Zhaomin Wu, Yanrong Wang & Feng Li

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  1. Yechuan Huang
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  2. Zhaomin Wu
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  3. Yanrong Wang
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  4. Feng Li
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Correspondence to Yechuan Huang.

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Huang, Y., Wu, Z., Wang, Y. et al. Examination of the effects of temperature and pressure on lipoxygenase activities in pork using response surface methodology. Food Sci Biotechnol 24, 1257–1263 (2015). https://doi.org/10.1007/s10068-015-0161-5

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  • DOI: https://doi.org/10.1007/s10068-015-0161-5

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