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A new RAPD-PCR based analytical assay for detection of sea bass and sea bream treated with ionizing radiation

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Abstract

Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to evaluate the effects of irradiation on the DNA of sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata). Meats of sea bass and sea bream were exposed to 0.25, 0.5, 1, 3, 5, and 7 kGy of irradiation (dosage rate 1.31 kGy/h) in a gamma cell at room temperature of approximately 10°C. RAPD-PCR was carried out using DNA samples of both control and irradiated groups. Gel electrophoresis showed loss of some amplicons, especially in the upper long bands of irradiated samples. Due to DNA damage caused by irradiation, the visibility of bands decreased as the radiation dosage increased. This study can be used as a basis for screening of irradiated samples based on loss of specific bands for a specific amount of irradiation.

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References

  1. Osterholm MT, Norgan AP. The role of irradiation in food safety. New Engl. J. Med. 350: 1898–1901 (2004)

    Article  CAS  Google Scholar 

  2. Parnes RB, Lichtenstein AH. Food irradiation: A safe and useful technology. J. Clin. Nutr. 7: 149–155 (2004)

    Google Scholar 

  3. Lee JL, Levin RE. New approach for discrimination of Vibrio vulnificus by real-time PCR before and after γ-irradiation. J. Microbiol. Meth. 73: 1–6 (2008)

    Article  CAS  Google Scholar 

  4. Goulas AE, Stahl M, Riganakos KA. Effect of different parameters on detection of irradiated fish and oregano using the ESR and PSL methods. Food Control 19: 1076–1085 (2008)

    Article  CAS  Google Scholar 

  5. Chung HW, Delincee H, Kwon JH. The application of different detection methods for irradiated dried anchovy and shrimp. Radiat. Phys. Chem. 63: 411–414 (2002)

    Article  CAS  Google Scholar 

  6. Kaya A. Biological effects of ionizing radiation. Dicle Univ. Med. J. 29: 3 (2002)

    Google Scholar 

  7. Villavicencio ALCH, Araujo MM, Marín-Huachaca NS, Mancini-Fiho J, Delincée H. Identification of irradiated refrigerated poultry with the DNA comet assay. Radiat. Phys. Chem. 71: 189–191 (2004)

    Article  CAS  Google Scholar 

  8. Lim S, Jung J, Kim M, Ryu S, Kim D. Effect of ionizing radiation on the quantitative detection of Salmonella using real-time PCR. Radiat. Phys. Chem. 77: 1112–1117 (2008)

    Article  CAS  Google Scholar 

  9. Arvanitoyannis IS. Irradiation of Commodities: Techniques, Applications, Detection, Legislation, Safety, and Consumer Opinion. Elsevier, Burlington, MA, USA. p. 710 (2010)

    Google Scholar 

  10. Arvanitoyannis IS, Stratakos A, Mante E. Impact of irradiation on fish seafood shelf life: A comprehensive review of application and irradiation detection. Crit. Rev. Food Sci. 49: 68–112 (2009)

    Article  Google Scholar 

  11. Duarte RC, Mozeika MA, Fanaro GB, Marchioni E, Villavicencio ALCH. DNA comet assay to identify different freezing temperatures of irradiated liver chicken. In: International Nuclear Atlantic Conference-INAC 2009. September 27 to October 2, Windsor Barra Hotel Convention Center, Rio de Janeiro, Brazil (2009)

    Google Scholar 

  12. Govan HL, Valles-Ayoub Y, Braun J. Fine mapping of DNA damage and repair in specific genomic segments. Nucleic Acids Res. 18: 3823–3830 (1990)

    Article  CAS  Google Scholar 

  13. Jennerwein MM, Eastman A. A polymerase chain reaction based method to detect cisplat in adducts in specific genes. Nucleic Acids Res. 19: 6209–6214 (1991)

    Article  CAS  Google Scholar 

  14. Theodorakis CW, Shugart LR. Genetic ecotoxicology II: Population genetic structure in mosquito fish exposed in situ to radionuclides. Ecotoxicology 6: 335–354 (1997)

    Article  CAS  Google Scholar 

  15. Becerril C, Ferrero, M, Sanz F, Castano A. Detection of mitomycin C-induced genetic damage in fish cells by use of RAPD. Mutagenesis 14: 449–456 (1999)

    Article  CAS  Google Scholar 

  16. Castano A, Becerril C. In vitro assessment of DNA damage after short-and long-term exposure to benzo(a)pyrene using RAPD and the RTG-2 fish cell line. Mutat. Res. 552: 141–151 (2004)

    Article  CAS  Google Scholar 

  17. Hagger JA, Atienzar FA, Jha AN. Genotoxic, cytotoxic, developmental, and survival effects of tritiated water in the early life stages of the marine mollusc, Mytilus edulis. Aquat. Toxicol. 74: 205–217 (2005)

    Article  CAS  Google Scholar 

  18. Atienzar FA, Cheung VV, Jha AN, Depledge MH. Fitness parameters and DNA effects are sensitive indicators of copper induced toxicity in Daphnia magna. Toxicol. Sci. 59: 241–250 (2001)

    Article  CAS  Google Scholar 

  19. Enan MR. Application of random amplified polymorphic DNA to detect genotoxic effect of heavy metals. Biotechnol. Appl. Bioc. 43: 147–154 (2006)

    Article  CAS  Google Scholar 

  20. Sutherland BM, Bennett PV, Sidorkina O, Laval J. Clustered DNA damages induced in isolated DNA and in human cells by low doses of ionizing radiation. P. Natl. Acad. Sci. 97: 103–108 (2000)

    Article  CAS  Google Scholar 

  21. Khan HM, Delincee H. Detection of irradiation treatment of foods using DNA ‘Comet Assay’. Radiat. Phys. Chem. 52: 141–144 (1998)

    Article  CAS  Google Scholar 

  22. Marín-Huachaca N, Delincée H, Mancini-Fiho J, Villavicencio ALCH. Use of the DNA comet assay to detect beef meat treated by ionizing radiation. Meat Sci. 71: 446–450 (2005)

    Article  Google Scholar 

  23. Erel Y, Yazici N, Ozvatan S, Ercin D, Cetinkaya N. Detection of irradiated quail meat by using DNA comet assays and evaluation of comets by image analysis. Radiat. Phys. Chem. 78: 776–781 (2009)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Bioengineering, Faculty of Engineering, Çanakkale Onsekiz Mart University, Çanakkale, Turkey

    Ergün Şakalar

  2. Department of Medical Biology, Faculty of Medicine, Gaziantep University, Gaziantep, Turkey

    Kaifee Arman

Authors
  1. Ergün Şakalar
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  2. Kaifee Arman
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Correspondence to Ergün Şakalar.

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Şakalar, E., Arman, K. A new RAPD-PCR based analytical assay for detection of sea bass and sea bream treated with ionizing radiation. Food Sci Biotechnol 24, 1233–1237 (2015). https://doi.org/10.1007/s10068-015-0158-0

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  • DOI: https://doi.org/10.1007/s10068-015-0158-0

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