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Effects of fucoidan on cell morphology and migration in osteoblasts

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Abstract

Fucoidan has been reported to induce the differentiation and mineralization of osteoblastic cells. Here we investigated the effects of fucoidan on the morphology and migration of osteoblasts and found that the effects of fucoidan on the migration of osteoblasts vary depending on the conditions under which the cells are treated. A migration assay using cells treated with fucoidan before stable attachment on plates showed slightly increased migration of fucoidan-treated cells compared to control cells, whereas wound healing experiments using stably attached cells exhibited a slightly decreased migration. The fucoidan-treated cells became shrunk and rounded and showed significantly decreased spreading and adhesion compared to control cells. F-actin was highly accumulated at the rounded cell cortex by fucoidan treatment. These studies suggest that the slightly increased migration of cells in the presence of fucoidan might be correlated to the morphological changes and decreased adhesion.

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References

  1. Zapopozhets TS, Besednova NN, Loenko IN. Antibacterial and immunomodulating activity of fucoidan. Antibiot. Khimioterap. 40: 9–13 (1995)

    CAS  Google Scholar 

  2. Wang J, Zhang Q, Zhang Z, Song H, Li P. Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica. Int. J. Biol. Macromol. 46: 6–12 (2010)

    Article  CAS  Google Scholar 

  3. Durig J, Bruhn T, Zurborn KH, Gutensohn K, Bruhn HD, Beress L. Anticoagulant fucoidan fractions from Fucus vesiculosus induce platelet activation in vitro. Thromb. Res. 85: 479–491 (1997)

    Article  CAS  Google Scholar 

  4. Nakamura T, Suzuki H, Wada Y, Kodama T, Doi T. Fucoidan induces nitric oxide production via p38 mitogen-activated protein kinase and NF-κB-dependent signaling pathways through macrophage scavenger receptors. Biochem. Bioph. Res. Co. 343: 286–294 (2006)

    Article  CAS  Google Scholar 

  5. Xue M, Ge Y, Zhang J, Liu Y, Wang Q, Hou L, Zheng Z. Fucoidan inhibited 4T1 mouse breast cancer cell growth in vivo and in vitro via downregulation of Wnt/β-catenin signaling. Nutr. Cancer 65: 460–468 (2013)

    Article  CAS  Google Scholar 

  6. Lee H, Kim JS, Kim E. Fucoidan from seaweed Fucus vesiculosus inhibits migration and invasion of human lung cancer cell via PI3K-Akt-mTOR pathways. PLoS ONE 7: e50624 (2012)

    Article  CAS  Google Scholar 

  7. Senthilkumar K, Manivasagan P, Venkatesan J, Kim SK. Brown seaweed fucoidan: Biological activity and apoptosis, growth signaling mechanism in cancer. Int. J. Biol. Macromol. 60: 366–374 (2013)

    Article  CAS  Google Scholar 

  8. Park SJ, Lee KW, Lim DS, Lee S. The sulfated polysaccharide fucoidan stimulates osteogenic differentiation of human adiposederived stem cells. Stem Cells Dev. 21: 2204–2211 (2012)

    Article  CAS  Google Scholar 

  9. Changotade SI, Korb G, Bassil J, Barroukh B, Willig C, Colliec-Jouault S, Durand P, Godeau G, Senni K. Potential effects of a low-molecular-weight fucoidan extracted from brown algae on bone biomaterial osteoconductive properties. J. Biomed. Mater. Res. A 87: 666–675 (2008)

    Article  Google Scholar 

  10. Cho YS, Jung WK, Kim JA, Choi IW, Kim SK. Beneficial effects of fucoidan on osteoblastic MG-63 cell differentiation. Food Chem. 116: 990–994 (2009)

    Article  CAS  Google Scholar 

  11. Jeong HS, Venkatesan J, Kim SK. Hydroxyapatite-fucoidan nanocomposites for bone tissue engineering. Int. J. Biol. Macromol. 57: 138–141 (2013)

    Article  CAS  Google Scholar 

  12. Dirckx N, Van Hul M, Maes C. Osteoblast recruitment to sites of bone formation in skeletal development, homeostasis, and regeneration. Birth Defects Res. C 99: 170–191 (2013)

    CAS  Google Scholar 

  13. Pignolo RJ, Kassem M. Circulating osteogenic cells: Implications for injury, repair, and regeneration. J. Bone Miner. Res. 26: 1685–1693 (2011)

    Article  CAS  Google Scholar 

  14. Nakahama K. Cellular communications in bone homeostasis and repair. Cell. Mol. Life Sci. 67: 4001–4009 (2010)

    Article  CAS  Google Scholar 

  15. Cho TM, Kim WJ, Moon SK. AKT signaling is involved in fucoidan-induced inhibition of growth and migration of human bladder cancer cells. Food Chem. Toxicol. 64: 344–352 (2014)

    Article  CAS  Google Scholar 

  16. Kim SH, Kang JH, Yang MP. Fucoidan directly regulates the chemotaxis of canine peripheral blood polymorphonuclear cells by activating F-actin polymerization. Vet. Immunol. Immunop. 151: 124–131 (2013)

    Article  CAS  Google Scholar 

  17. Kucik DF, Wu C. Cell-adhesion assays. Methods Mol. Biol. 294: 43–54 (2005)

    CAS  Google Scholar 

  18. Jeon TJ, Lee DJ, Merlot S, Weeks G, Firtel RA. Rap1 controls cell adhesion and cell motility through the regulation of myosin II. J. Cell Biol. 176: 1021–1033 (2007)

    Article  CAS  Google Scholar 

  19. Boo HJ, Hong JY, Kim SC, Kang JI, Kim MK, Kim EJ, Hyun JW, Koh YS, Yoo ES, Kwon JM, Kang HK. The anticancer effect of fucoidan in PC-3 prostate cancer cells. Mar. Drugs 11: 2982–2999 (2013)

    Article  Google Scholar 

  20. Park HS, Hwang HJ, Kim GY, Cha HJ, Kim WJ, Kim ND, Yoo YH, Choi YH. Induction of apoptosis by fucoidan in human leukemia U937 cells through activation of p38 MAPK and modulation of Bcl-2 family. Mar. Drugs 11: 2347–2364 (2013)

    Article  Google Scholar 

  21. Insall R. The interaction between pseudopods and extracellular signalling during chemotaxis and directed migration. Curr. Opin. Cell Biol. 25: 526–531 (2013)

    Article  CAS  Google Scholar 

  22. Lee MR, Jeon TJ. Cell migration: Regulation of cytoskeleton by Rap1 in Dictyostelium discoideum. J. Microbiol. 50: 555–561 (2012)

    Article  Google Scholar 

  23. Ridley AJ, Schwartz MA, Burridge K, Firtel RA, Ginsberg MH, Borisy G, Parsons JT, Horwitz AR. Cell migration: Integrating signals from front to back. Science 302: 1704–1709 (2003)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Life Science & BK21- Plus Research Team for Bioactive Control Technology, College of Natural Sciences, Chosun University, Gwangju, 501-759, Korea

    Hyeseon Kim, Ara Lee & Taeck J. Jeon

  2. Department of Biomedical Engineering, & BK21-Plus Research Center for Marine-Integrated Biomedical Technology, Pukyong National University, Busan, 608-737, Korea

    Won-Kyo Jung

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  1. Hyeseon Kim
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  2. Ara Lee
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  3. Won-Kyo Jung
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  4. Taeck J. Jeon
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Correspondence to Taeck J. Jeon.

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Kim, H., Lee, A., Jung, WK. et al. Effects of fucoidan on cell morphology and migration in osteoblasts. Food Sci Biotechnol 24, 699–704 (2015). https://doi.org/10.1007/s10068-015-0091-2

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  • DOI: https://doi.org/10.1007/s10068-015-0091-2

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