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Angiotensin-I converting enzyme fatty acid inhibitory fractions from the Korean melania snail Semisulcospira coreana

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Abstract

The angiotensin-I converting enzyme (ACE) inhibitory activity of Semisulcospira coreana was investigated. Crude CH2Cl2 and MeOH extracts showed 59.3 and 25.6% ACE-inhibitory activities at 5 mg/mL, respectively. After 2 phase separations including n-hexane, 85% aqueous MeOH, n-BuOH, and H2O, the 85% aq. MeOH fraction exhibited the highest ACE-inhibitory activity of 62.1%. The inhibitory activity of 85% aq. EtOH subfraciton 5 (merf5) partially purified using C18 reversed-phase flash chromatography was increased up to 94.6% at 0.5 mg/mL. Typical fatty acids signals observed in 1H NMR and gas chromatography analysis of merf5 and n-BuOH subfractions 6 (burf6) indicated that components of merf5 and burf6 included fatty acids. The fatty acid fractions from S. coreana act as novel ACE inhibitors.

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Author notes

  1. You Ah Kim

    Present address: Bio Materials R&D Center, Kolmar Korea Co. Ltd., Cheongju, Chungbuk, 363-883, Korea

Authors and Affiliations

  1. Aquaculture Industry Division, SSFRI, National Fisheries Research & Development Institute (NFRDI), Yeosu, Jeonnam, 556-823, Korea

    Yeon-Kye Kim, Soon Bum Shin & Tae Seek Lee

  2. Food Safety Research Division, National Fisheries Research & Development Institute (NFRDI), Busan, 619-705, Korea

    You Ah Kim & Ho-Dong Yoon

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  1. Yeon-Kye Kim
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  2. You Ah Kim
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Correspondence to Yeon-Kye Kim.

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Kim, YK., Kim, Y.A., Shin, S.B. et al. Angiotensin-I converting enzyme fatty acid inhibitory fractions from the Korean melania snail Semisulcospira coreana . Food Sci Biotechnol 24, 681–688 (2015). https://doi.org/10.1007/s10068-015-0089-9

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  • DOI: https://doi.org/10.1007/s10068-015-0089-9

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