Skip to main content
SpringerLink
Log in

Chemical characterization and antitumor activity of an exopolysaccharide from Pholiota Squarrosa Quel. AS 5.245

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

A water-soluble exopolysaccharide (PESP-1) was extracted and purified using DEAE Sephadex A-50 and Sephadex G-100 columns from a submerged culture broth of Pholiota squarrosa Quel. AS 5.245. PEPS-1 was investigated for antitumor activity against Heps tumors implanted in mice. An inhibition rate of 78.46% at a dosage of 100 mg/kg was observed. PEPS-1 significantly (p<0.05) increased the relative spleen/thymus indices of Heps tumor-bearing mice at a dosage of 100 mg/kg, compared with controls. Antitumor properties were probably related to stimulation of the immune response. Preliminary physicochemical analysis identified PEPS-1 as a heteropolysaccharide mainly containing d-mannose, d-glucose and d-galactose at molar ratios of 50:33:18. Small amounts of d-rhamnose and d-xylose were also detected. The average Mw of PEPS-1 was 3.26×104 Da. Structural features probably played an important role in the antitumor activity of PEPS-1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. World Health Organization Media Center. Fact sheet N° 297 on cancer. Available from: http://www.who.int/mediacentre/factsheets/fs297/en/. Accessed Nov. 1, 2014.

    Google Scholar 

  2. Suárez ER, Kralovec JA. Grindley TB. Isolation of phosphorylated polysaccharides from algae: The immunostimulatory principle of Chlorella pyrenoidosa. Carbohyd. Res. 345: 1190–1204 (2010)

    Article  Google Scholar 

  3. Cai ZB, Li W, Wang HT, Yan WQ, Zhou YL, Wang GJ, Cui JW, Wang F. Antitumor effects of a purified polysaccharide from Rhodiola rosea and its action mechanism. Carbohyd. Polym. 90: 296–300 (2012)

    Article  CAS  Google Scholar 

  4. Ahrazem O, Prieto A, Giméńez-Abián MI, Leal JA, Jiménez-Barbero J, Bernabé M. Structural elucidation of fungal polysaccharides isolated from the cell wall of Plectosphaerella cucumerina and Verticillium spp. Carbohyd. Res. 341: 246–252 (2006)

    Article  CAS  Google Scholar 

  5. Wasser SP. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl. Microbiol. Biot. 60: 258–274 (2002)

    Article  CAS  Google Scholar 

  6. Kües U, Liu Y. Fruiting body production in basidiomycetes. Appl. Microbiol. Biot. 54: 141–152 (2000)

    Article  Google Scholar 

  7. Yu CH, Kan SF, Shu CH, Lu TJ, Sun-Hwang L, Wang PS. Inhibitory mechanisms of Agaricus blazei Murill on the growth of prostate cancer in vitro and in vivo. J. Nutr. Biochem. 20: 753–764 (2009)

    Article  CAS  Google Scholar 

  8. Guo L, Xie JH, Ruan YY, Zhou L, Zhu HY, Yun XJ, Jiang Y, Lü L, Chen KL, Min ZH, Wen YM, Gu JX. Characterization and immunostimulatory activity of a polysaccharide from the spores of Ganoderma lucidum. Int. Immunopharmacol. 9: 1175–1182 (2009)

    Article  CAS  Google Scholar 

  9. Yu ZH, Yin LH, Yang Q, Liu Y. Effect of Lentinus edodes polysaccharide on oxidative stress, immunity activity and oral ulceration of rats stimulated by phenol. Carbohyd. Polym. 75: 115–118 (2009)

    Article  CAS  Google Scholar 

  10. Tian EJ. Studies on taxonomy of the genus Pholiota from China. MS thesis, Jilin Agricultural University, Jinlin, China (2006)

    Google Scholar 

  11. JT Xu. Chinese Medical Mycology. China Medical University-Peking Union Medical Joint Press, Beijing, China. pp. 436–437 (1997)

    Google Scholar 

  12. Staub AM. Removal of proteins: Sevag method. pp. 5–6. In: Methods in Carbohydrate Chemistry. Whistler RL (ed). Academic Press, Inc., New York, NY, USA (1965)

    Google Scholar 

  13. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350–356 (1956)

    Article  CAS  Google Scholar 

  14. Committee for the Update of the Guide for the Care and Use of Laboratory Animals; National Research Council. Guide for the Care and Use of Laboratory Animals. 8th ed. The National Academies Press, Washington DC, USA. pp. 41–75 (2011)

    Google Scholar 

  15. Shi L, Fu YL. Isolation, purification, and immunomodulatory activity in vitro of three polysaccharides from roots of Cudrania tricuspidata. Acta Bioch. Bioph. Sin. 43: 418–424 (2011)

    Article  CAS  Google Scholar 

  16. Li H, Wang S. Kinetics of inhibition of ribonuclease A by Pholiota Nameko polysaccharide. Int. J. Biol. Macromol. 40: 134–138 (2007)

    Article  CAS  Google Scholar 

  17. Ren L, Perera C, Hemar Y. Antitumor activity of mushroom polysaccharides: A review. Food Funct. 3: 1118–1130 (2012)

    Article  CAS  Google Scholar 

  18. Pang X, Chen Z, Gao X, Liu W, Slavin M, Yao W, Yu LL. Potential of a novel polysaccharide preparation (GLPP) from Anhui-grown Ganoderma lucidum in tumor treatment and immunostimulation. J. Food Sci. 72: S435–S442 (2007)

    Article  CAS  Google Scholar 

  19. Surenjav U, Zhang LN, Xu XJ, Zhang XF, Zeng FB. Effects of molecular structure on antitumor activities of (1/3)-β-d-glucans from different Lentinus Edodes. Carbohyd. Polym. 63: 97–104 (2006)

    Article  CAS  Google Scholar 

  20. Chen X, Nie W, Yu G, Li Y, Hu Y, Lu J, Jin L. Antitumor and immunomodulatory activity of polysaccharides from Sargassum fusiforme. Food Chem. Toxicol. 50: 695–700 (2012)

    Article  CAS  Google Scholar 

  21. Wang Y, Zhao H, Miao XL, Liu D, Jiang HX, Liu PP, Wang YF, Yin HP. Structural determination and antitumor activities of a watersoluble polysaccharide from Mortierella hepialid. Fitoterapia 86: 13–18 (2013)

    Article  CAS  Google Scholar 

  22. Wu X, Mao G, Fan Q, Zhao T, Zhao J, Li F, Yang L. Isolation, purification, immunological, and anti-tumor activities of polysaccharides from Gymnema sylvestre. Food Res. Int. 48: 935–939 (2012)

    Article  CAS  Google Scholar 

  23. Wang JG, Wang YT, Liu XB, Yuan YH, Yue TL. Free radical scavenging and immunomodulatory activities of Ganoderma lucidum polysaccharides derivatives. Carbohyd. Polym. 91: 33–38 (2013)

    Article  CAS  Google Scholar 

  24. Wang ZH, Wu BJ, Zhang XH, Xu M, Chang HM, Lu XY, Ren XY. Purification of a polysaccharide from Boschniakia rossica and its synergistic antitumor effect combined with 5-fluorouracil. Carbohyd. Polym. 89: 31–35 (2012)

    Article  Google Scholar 

  25. Longley DB, Harkin DP, Johnston PG. 5-Fluorouracil: Mechanisms of action and clinical strategies. Nat. Rev. Cancer 3: 330–338 (2003)

    Article  CAS  Google Scholar 

  26. Ooi VE, Liu F. Immunomodulation and anti-cancer activity of polysaccharide-protein complexes. Curr. Med. Chem. 7: 715–729 (2000)

    Article  CAS  Google Scholar 

  27. Shi X, Zhao Y, Jiao Y, Shi T, Yang X. XROS-Dependent mitochondria molecular mechanisms underlying antitumor activity of Pleurotus abalonus acidic polysaccharides in human breast cancer MCF-7 cells. PLoS ONE 8: e64266 (2013)

    Article  CAS  Google Scholar 

  28. Mizuno T. A development of antitumor polysaccharides from mushroom fungi. Food Ingred. J. Jpn. 167: 69–85 (1996)

    CAS  Google Scholar 

  29. Tsukagoshi S, Hashimoto Y, Fujii G, Kobayashi H, Nomoto K, Orita K. Krestin (PSK). Cancer Treat. Rev. 11: 131–155 (1984)

    Article  CAS  Google Scholar 

  30. Gao QP, Seljelid R, Chen HQ, Jiang R. Characterization of acidic heteroglycans from Tremella fuciformis Berk with cytokine stimulating activity. Carbohyd. Res. 288: 135–142 (1996)

    Article  CAS  Google Scholar 

  31. Wang JM, Jiao Y, Lu FX, Bie XM, Wang Y, Lu ZX. Study on activity of mycelium proteins from Pholiota squarrosa. J. Acta Agric. Jiangxi 20: 103–105 (2008)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China

    Haizhen Zhao, Juan Wang, Fengxia Lv, Xiaomei Bie & Zhaoxin Lu

Authors
  1. Haizhen Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Juan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fengxia Lv
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiaomei Bie
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhaoxin Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhaoxin Lu.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhao, H., Wang, J., Lv, F. et al. Chemical characterization and antitumor activity of an exopolysaccharide from Pholiota Squarrosa Quel. AS 5.245. Food Sci Biotechnol 24, 659–664 (2015). https://doi.org/10.1007/s10068-015-0086-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-015-0086-z

Keywords

Search

Navigation