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Protective effects of new blackberry cultivar MNU-32 extracts against H2O2-induced oxidative stress in HepG2 cells

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Abstract

Protective effects of extracts of the new cultivar MNU-32 developed from blackberry (Rubus fruticosus) cultivar V-9 against H2O2-induced oxidative stress in HepG2 cells were investigated. DPPH radical scavenging activities of MNU-32 and V-9 extracts were 570.00 μg/mL (IC50) and 644.55 μg/mL (IC50), respectively. Inhibition effects of MNU-32 extracts against intracellular ROS generation, cell cytotoxicity, and lactate dehydrogenase release were higher than for the V-9 extract in H2O2-treated HepG2 cells. Compared with the V-9 extract, MNU-32 extract treatments produced higher levels of lipid peroxidation attenuation and greater protection against DNA damage, restored the activity of superoxide dismutase in H2O2-treated HepG2 cells, and increased protein expression levels of superoxide dismutase in HepG2 cells in a dose and time-dependent manner. MNU-32 blackberry extracts exerted protective effects against H2O2-induced cell damage due to higher anthocyanin contents than the V-9 blackberry extract.

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Authors and Affiliations

  1. Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, 580-185, Korea

    Byoung-Ok Cho, Chang-Hyun Jin, Jaihyunk Ryu, Dong-Sub Kim, Si Yong Kang & Il-Yun Jeong

  2. Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang, Chungbuk, 363-883, Korea

    Hyung-Won Ryu

  3. Department of Food Science and Human Nutrition, Chungnam National University, Daejeon, 305-764, Korea

    Chang-Wook Lee & Hong-Sun Yook

  4. Functional Crop Resource Development Division, National Institute of Crop Science, RDA, Miryang, Gyeongnam, 627-803, Korea

    Woo-Duck Seo

Authors
  1. Byoung-Ok Cho
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  2. Hyung-Won Ryu
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  3. Chang-Wook Lee
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  4. Chang-Hyun Jin
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  6. Jaihyunk Ryu
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  7. Dong-Sub Kim
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  8. Si Yong Kang
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  10. Il-Yun Jeong
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Correspondence to Il-Yun Jeong.

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Cho, BO., Ryu, HW., Lee, CW. et al. Protective effects of new blackberry cultivar MNU-32 extracts against H2O2-induced oxidative stress in HepG2 cells. Food Sci Biotechnol 24, 643–650 (2015). https://doi.org/10.1007/s10068-015-0084-1

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  • DOI: https://doi.org/10.1007/s10068-015-0084-1

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