Growth-inhibiting effects and chemical composition of essential oils extracted from Platycladus orientalis leaves and stems toward human intestinal bacteria

Abstract

The growth-inhibiting activities of the essential oils extracted from Platycladus orientalis leaves and stems were examined against intestinal bacteria. The leaf oil exhibited a strong response against Clostridium difficile and C. perfringens at 5.0 mg/disc, but the stem oil showed no inhibitory activity against intestinal bacteria. The oils from P. orientalis leaves and stems were analyzed by GC-Mass spectroscopy. The main constituents of the essential oil in P. orientalis leaves: α-pinene (18.5%), cedrene (13.6%), α-cedrol (11.3%), β-caryophyllene (10.2%), 3-carene (8.2%), and α-terpinyl acetate (5.1%), and in the stems: α-pinene (17.7%), 3-carene (16.3%), β-caryophyllene (13.4%), β-phellandrene (9.5%), β-selinene (6.5%), and α-cedrol (6.1%). The cedrene isomers showed significant (33.1–34.7 mm) inhibitory effect against C. difficile and C. perfringens and moderate inhibitory effect against Escherichia coli at 2.0 mg/disc. The oil of P. orientalis leaves, in which cedrene isomers are the major component, could be used as a selective inhibitor against harmful bacteria.

This is a preview of subscription content, access via your institution.

References

  1. 1.

    Guarner F, Malagelada JR. Gut flora in health and disease. Lancet 361: 512–519 (2003)

    Article  Google Scholar 

  2. 2.

    Conte MP, Schippa S, Zamboni I, Penta M, Chiarini F, Seganti L, Osborrn J, Falconieri P, Borrelli O, Cucchiara S. Gut-associated bacterial microbiota in paediatric patients with inflammatory bowel disease. Gut 55: 1760–1767 (2006)

    Article  CAS  Google Scholar 

  3. 3.

    Nord CE, Rasmanis G, Wahlund E. Effect of dalbavancin on the normal intestinal microflora. J. Antimicrob. Chemoth. 58: 627–631 (2006)

    Article  CAS  Google Scholar 

  4. 4.

    Beaugerie L, Petit JC. Antibiotic-associated diarrhea. Best Pract. Res. Cl. Ga. 18: 337–352 (2004)

    Article  CAS  Google Scholar 

  5. 5.

    Kim MG, Lee HS. Growth-inhibiting activities of phenethyl isothiocyante and its derivatives against intestinal bacteria. J. Food Sci. 74: M467–471 (2009)

    Article  CAS  Google Scholar 

  6. 6.

    Jeong EY, Jeon JH, Kim HW, Kim MG, Lee HS. Antimicrobial activity of leptospermone and its derivatives against human intestinal bacteria. Food Chem. 115: 1401–1404 (2009)

    Article  CAS  Google Scholar 

  7. 7.

    Lee HS, Ahn YJ. Growth-inhibiting effects of Cinnamomum cassia bark-derived materials on human intestinal bacteria. J. Agr. Food Chem. 46: 8–12 (1998)

    Article  CAS  Google Scholar 

  8. 8.

    Choi JW, Cho EJ, Lee DG, Choi K, Ku JJ, Park KW, Lee HS. Antibacterial activity of triterpenoids from Clerodendron trichotomum. J. Appl. Biol. Chem. 55: 169–172 (2012)

    Article  CAS  Google Scholar 

  9. 9.

    Lee YJ, Hwang SM, Yoon JJ, Lee SM, Kyung EH, Kim JS, Kang DG, Lee HS. Inhibitory effect of Thuja orientalis on TNF-α-induced vascular inflammation. Phytother. Res. 24: 1489–1495 (2010)

    Article  Google Scholar 

  10. 10.

    Lee HS. Tyrosinase inbibitors of Pulsatilla cernua root-derived materials. J. Agr. Food Chem. 50: 1400–1403 (2002)

    Article  CAS  Google Scholar 

  11. 11.

    Lee HS. Acaricidal activity of constituents identified in Foeniculum vulgare fruit oil against Dermatophagoides spp. (Acari: Pytoglyphidae). J. Agr. Food Chem. 52: 2887–2889 (2004)

    Article  CAS  Google Scholar 

  12. 12.

    Lei H, Wang Y, Liang F, Su W, Feng Y, Guo X, Wang N. Composition and variability of essential oils of Platycladus orientalis growing in China. Biochem. Syst. Ecol. 38: 1000–1006 (2010)

    Article  CAS  Google Scholar 

  13. 13.

    Guleria S, Kumar A, Tiku AK. Chemical composition and fungitoxic activity of essential oil of Thuja orientalis L. grown in the north-western Himalaya. Z. Naturforsch. C 63: 211–214 (2008)

    Article  CAS  Google Scholar 

  14. 14.

    Jeon JH, Lee SH, Kim MK, Lee HS. Larvicidal activity of Chamaecyparis obtuse and Thuja orientalis leaf oils against two mosquito species. J. Korean Soc. Appl. Biol. Chem. 48: 26–28 (2005)

    CAS  Google Scholar 

  15. 15.

    Yang JY, Cho KS, Chung NH, Kim CH, Suh JW, Lee HS. Constituents of volatile compounds derived from Melaleuca alternifolia leaf oil and acaricidal toxicities against house dust mites. J. Korean Soc. Appl. Biol. Chem. 56: 91–94 (2013)

    Article  CAS  Google Scholar 

  16. 16.

    Zhou Z, Liu X, Yang F, Wang L. Comparison of chemical composition of essential oil from Platycladus orientalis by supercritical fluid extraction and steam distillation extraction. Med. Eng. Bioinform. 19: 219–224 (2014)

    Article  Google Scholar 

  17. 17.

    Hashemi SM, Safavi SA. Chemical constituents and toxicity of essential oils of oriental arborvitae, Platycladus orientalis (L.) Franco, against three stored-product beetles. Chil. J. Agr. Res. 72: 188–194 (2012)

    Article  Google Scholar 

  18. 18.

    Afsharypuor S, Nayebzadeh B. Essential oil constituents of young stem, leaf and fruit of Platycladus orientalis (L.) Franco grown in Isfahan (Iran). J. Essent. Oil Res. 21: 525–528 (2009)

    Article  CAS  Google Scholar 

  19. 19.

    Valentines MC, Vilaplana R, Torres R, Usall J, Larrigaudiere C. Specific roles of enzymatic browning and lignification in apple disease resistance. Postharvest Biol. Tec. 36: 227–234 (2005)

    Article  CAS  Google Scholar 

  20. 20.

    Hassanzadeh MK, Rahimizadeh M, Bazzaz BSF, Emami SA, Assili J. Chemical and antimicrobial studies of Platycladus orientalis essential oils. Pharm Biol. 39: 388–390 (2001)

    Article  CAS  Google Scholar 

  21. 21.

    Ngan LTM, Moon JK, Kim JH, Shibamoto T, Ahn YJ. Growth-inhibiting effects of Paeonia lactiflora root steam distillate constituents and structurally related compounds on human intestinal bacteria. World J. Microb. Biot. 28: 1575–1583 (2012)

    Article  CAS  Google Scholar 

  22. 22.

    Carson CF, Hammer KA, Riley TV. Melaleuca alternifolia (Tea tree) oil: A review of antimicrobial and other medicinal properties. Clin. Microbiol. Rev. 19: 50–62 (2006)

    Article  CAS  Google Scholar 

  23. 23.

    Asili J, Rard MR, Ahi A, Emami SA. Chemical composition of the essential oil from aerial parts of Haplophyllum acutifolium (DC.) G. Don from Iran. J. Essent. Oil Bear Pl. 14: 201–207 (2011)

    Article  CAS  Google Scholar 

  24. 24.

    Kim MK, Kim YM, Lee HS. Growth-inhibiting effects of Juniperus virginiana leaf-extracted components toward human intestinal bacteria. Food Sci. Biotechnol. 14: 164–167 (2005)

    CAS  Google Scholar 

  25. 25.

    Murugesan S, Rajeshkannan C, Sumathi R, Manivachakam P, Babu DS. Bioactivity of root hexane extract of Coleus forskohlii Briq. Laviatae: GC/MS/MS characterization and identification. Eur. J. Exp. Biol. 2: 1469–1473 (2012)

    CAS  Google Scholar 

  26. 26.

    Sankahya NB, Kayce P, Halay E, Göktürk RS, Sümbül H, Kirmizigül S. Phytochemical analysis of the essential oils of 10 endemic Cephalaria species from Turkey. Nat. Prod. Res. 27: 830–833 (2013)

    Article  Google Scholar 

  27. 27.

    Su YC, Hsu KP, Wang EI, Ho CL. Composition, anticancer, and antimicrobial activities in vitro of the heartwood essential oil of Cunninghamia lanceolata var. konishii from Taiwan. Nat. Prod. Commun. 7: 1245–1247 (2012)

    CAS  Google Scholar 

  28. 28.

    Mastelic J, Politeo O, Jerkovic I, Radosevic N. Composition and antimicrobial activity of Helichrysum italicum essential oil and its terpene and terpenoid fractions. Chem. Nat. Compd. 41: 35–40 (2005)

    Article  CAS  Google Scholar 

  29. 29.

    Nibret E, Wink M. Trypanocidal and antileukaemic effects of the essential oils of Hagenia abyssinica, Leonotis ocymifolia, Moringa stenopetala, and their main individual constituents. Phytomedicine 17: 911–920 (2010)

    Article  CAS  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Hoi-Seon Lee.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Kim, MG., Lee, HS. Growth-inhibiting effects and chemical composition of essential oils extracted from Platycladus orientalis leaves and stems toward human intestinal bacteria. Food Sci Biotechnol 24, 427–431 (2015). https://doi.org/10.1007/s10068-015-0056-5

Download citation

Keywords

  • α-cedrene
  • β-cedrene
  • growth inhibition
  • intestinal bacteria
  • Platycladus orientalis