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Growth-inhibiting effects and chemical composition of essential oils extracted from Platycladus orientalis leaves and stems toward human intestinal bacteria

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Abstract

The growth-inhibiting activities of the essential oils extracted from Platycladus orientalis leaves and stems were examined against intestinal bacteria. The leaf oil exhibited a strong response against Clostridium difficile and C. perfringens at 5.0 mg/disc, but the stem oil showed no inhibitory activity against intestinal bacteria. The oils from P. orientalis leaves and stems were analyzed by GC-Mass spectroscopy. The main constituents of the essential oil in P. orientalis leaves: α-pinene (18.5%), cedrene (13.6%), α-cedrol (11.3%), β-caryophyllene (10.2%), 3-carene (8.2%), and α-terpinyl acetate (5.1%), and in the stems: α-pinene (17.7%), 3-carene (16.3%), β-caryophyllene (13.4%), β-phellandrene (9.5%), β-selinene (6.5%), and α-cedrol (6.1%). The cedrene isomers showed significant (33.1–34.7 mm) inhibitory effect against C. difficile and C. perfringens and moderate inhibitory effect against Escherichia coli at 2.0 mg/disc. The oil of P. orientalis leaves, in which cedrene isomers are the major component, could be used as a selective inhibitor against harmful bacteria.

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  1. Department of Bioenvironmental Chemistry and Institute of Agricultural Science & Technology, College of Agriculture & Life Science, Chonbuk National University, Jeonju, Jeonbuk, 561-756, Korea

    Min-Gi Kim & Hoi-Seon Lee

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  1. Min-Gi Kim
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  2. Hoi-Seon Lee
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Correspondence to Hoi-Seon Lee.

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Kim, MG., Lee, HS. Growth-inhibiting effects and chemical composition of essential oils extracted from Platycladus orientalis leaves and stems toward human intestinal bacteria. Food Sci Biotechnol 24, 427–431 (2015). https://doi.org/10.1007/s10068-015-0056-5

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  • DOI: https://doi.org/10.1007/s10068-015-0056-5

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