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Growth-inhibiting effects and chemical composition of essential oils extracted from Platycladus orientalis leaves and stems toward human intestinal bacteria

Food Science and Biotechnology volume 24, pages 427–431 (2015)Cite this article

Abstract

The growth-inhibiting activities of the essential oils extracted from Platycladus orientalis leaves and stems were examined against intestinal bacteria. The leaf oil exhibited a strong response against Clostridium difficile and C. perfringens at 5.0 mg/disc, but the stem oil showed no inhibitory activity against intestinal bacteria. The oils from P. orientalis leaves and stems were analyzed by GC-Mass spectroscopy. The main constituents of the essential oil in P. orientalis leaves: α-pinene (18.5%), cedrene (13.6%), α-cedrol (11.3%), β-caryophyllene (10.2%), 3-carene (8.2%), and α-terpinyl acetate (5.1%), and in the stems: α-pinene (17.7%), 3-carene (16.3%), β-caryophyllene (13.4%), β-phellandrene (9.5%), β-selinene (6.5%), and α-cedrol (6.1%). The cedrene isomers showed significant (33.1–34.7 mm) inhibitory effect against C. difficile and C. perfringens and moderate inhibitory effect against Escherichia coli at 2.0 mg/disc. The oil of P. orientalis leaves, in which cedrene isomers are the major component, could be used as a selective inhibitor against harmful bacteria.

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Authors and Affiliations

  1. Department of Bioenvironmental Chemistry and Institute of Agricultural Science & Technology, College of Agriculture & Life Science, Chonbuk National University, Jeonju, Jeonbuk, 561-756, Korea

    Min-Gi Kim & Hoi-Seon Lee

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  1. Min-Gi Kim
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  2. Hoi-Seon Lee
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Correspondence to Hoi-Seon Lee.

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Kim, MG., Lee, HS. Growth-inhibiting effects and chemical composition of essential oils extracted from Platycladus orientalis leaves and stems toward human intestinal bacteria. Food Sci Biotechnol 24, 427–431 (2015). https://doi.org/10.1007/s10068-015-0056-5

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  • DOI: https://doi.org/10.1007/s10068-015-0056-5

Keywords

  • α-cedrene
  • β-cedrene
  • growth inhibition
  • intestinal bacteria
  • Platycladus orientalis