Skip to main content

Advertisement

Log in

Polyphenolic contents and the effects of methanol extracts from mango varieties on breast cancer cells

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

Bioactivities of peel and flesh extracts of 3 genetically diverse mango (Mangifera indica L.) varieties were studied. Nam Doc Mai peel extracts, containing the largest amounts of polyphenols, were associated with an effect on MCF-7 viable cell numbers with an IC50 (dose required for 50% inhibition of cell viability) of 56 μg/mL and significantly (p<0.01) induced cell death in MDA-MB-231 cells, compared with other varieties. Hydrophilic fractions of Nam Doc Mai peel extracts had the highest bioactivity values against both MCF-7 and MDA-MB-231 cells. Soluble polyphenols were present in the largest amounts in most hydrophilic fractions. The Nam Doc Mai mango variety contains high levels of fruit peel bioactivity, which appears to be related to the nature of the polyphenol composition.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Peer WA, Murphy AS. Flavonoids as signal molecules: Targets of flavonoid action. pp. 239–268. In: The Science of Flavonoids. Grotewold E (ed). Springer, New York, NY, USA (2006)

    Chapter  Google Scholar 

  2. Szajdek A, Borowska EJ. Bioactive compounds and health-promoting properties of berry fruits: A review. Plant Food. Hum. Nutr. 63: 147–156 (2008)

    Article  CAS  Google Scholar 

  3. Hollman P, Katan M. Absorption, metabolism, and health effects of dietary flavonoids in man. Biomed. Pharmacother. 51: 305–310 (1997)

    Article  CAS  Google Scholar 

  4. Liu RH. Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. Am. J. Clin. Nutr. 78: 517S–520S (2003)

    CAS  Google Scholar 

  5. Smith-Warner SA, Genkinger J, Giovannucci E. Fruit and vegetable consumption and cancer. pp. 97–174. In: Nutritional Oncology. Heber D, Blackburn GL, Go CLW, Milner JA (eds). Elsevier, Inc., San Diego, CA, USA (2006)

    Chapter  Google Scholar 

  6. Bettuzzi S, Brausi M, Rizzi F, Castagnetti G, Peracchia G, Corti A. Chemoprevention of human prostate cancer by oral administration of green tea catechins in volunteers with high-grade prostate intraepithelial neoplasia: A preliminary report from a one-year proof-of-principle study. Cancer Res. 66: 1234–1240 (2006)

    Article  CAS  Google Scholar 

  7. Russo M, Palumbo R, Tedesco I, Mazzarella G, Russo P, Iacomino G, Russo GL. Quercetin and anti-CD95 (Fas/Apo1) enhance apoptosis in HPB-ALL cell line. FEBS Lett. 462: 322–328 (1999)

    Article  CAS  Google Scholar 

  8. Arai Y, Watanabe S, Kimira M, Shimoi K, Mochizuki R, Kinae N. Dietary intakes of flavonols, flavones and isoflavones by japanese women and the inverse correlation between quercetin intake and plasma ldl cholesterol concentration. J. Nutr. 130: 2243–2250 (2000)

    CAS  Google Scholar 

  9. Molina M, Sanchez-Reus I, Iglesias I, Benedi J. Quercetin, a flavonoid antioxidant, prevents and protects against ethanol-induced oxidative stress in mouse liver. Biol. Pharm. Bull. 26: 1398–1402 (2003)

    Article  CAS  Google Scholar 

  10. Wang SY, Lin HS. Antioxidant activity in fruits and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage. J. Agr. Food Chem. 48: 140–146 (2000)

    Article  CAS  Google Scholar 

  11. Bally ISE. Mangifera indica (mango). pp. 1–26. In: Specialty Crops for Pacific Islands. Elevitch CR (ed). Permanent Agriculture Resources, Holualoa, HI, USA (2006)

    Google Scholar 

  12. Masibo M, He Q. Major mango polyphenols and their potential significance to human health. Compr. Rev. Food Sci. F. 7: 309–319 (2008)

    Article  Google Scholar 

  13. Ajila CM, Bhat SG, Rao UJSP. Valuable components of raw and ripe peels from two Indian mango varieties. Food Chem. 102: 1006–1011 (2007)

    Article  CAS  Google Scholar 

  14. Masibo M, He Q. Mango bioactive compounds and related nutraceutical properties. Food Rev. Int. 25: 346–370 (2009)

    Article  CAS  Google Scholar 

  15. Gorinstein S, Zemser M, Haruenkit R, Chuthakorn R, Grauer F, Martin-Belloso O, Trakhtenberg S. Comparative content of total polyphenols and dietary fiber in tropical fruits and persimmon. J. Nutr. Biochem. 10: 367–371 (1999)

    Article  CAS  Google Scholar 

  16. Berardini N, Knodler M, Schieber A, Carle R. Utilization of mango peels as a source of pectin and polyphenolics. Innov. Food. Sci. Emerg. 6: 442–452 (2005)

    Article  CAS  Google Scholar 

  17. Ribeiro SMR, Barbosa LCA, Queiroz JH, Knödler M, Schieber A. Phenolic compounds and antioxidant capacity of Brazilian mango (Mangifera indica L.) varieties. Food Chem. 110: 620–626 (2008)

    Article  CAS  Google Scholar 

  18. Rocha RSM, Queiroz JH, Lopes RdQME, Campos FM, Pinheiro SaHM. Antioxidant in mango (Mangifera indica L.) pulp. Plant Food. Hum. Nutr. 62: 13–17 (2007)

    Article  Google Scholar 

  19. Kang TB, Liang NC. Studies on the inhibitory effects of quercetin on the growth of HL-60 leukemia cells. Biochem. Pharmacol. 54: 1013–1018 (1997)

    Article  CAS  Google Scholar 

  20. Rajendran P, Ekambaram G, Sakthisekaran D. Effect of mangiferin on benzo(a)pyrene induced lung carcinogenesis in experimental Swiss albino mice. Nat. Prod. Res. 22: 672–680 (2008)

    Article  CAS  Google Scholar 

  21. Percival SS, Talcott ST, Chin ST, Mallak AC, Lounds-Singleton A, Pettit-Moore J. Neoplastic transformation of BALB/3T3 cells and cell cycle of HL-60 cells are inhibited by mango (Mangifera indica L.) juice and mango juice extracts. J. Nutr. 136: 1300–1304 (2006)

    CAS  Google Scholar 

  22. Prasad S, Madan E, Nigam N, Roy P, George J, Shukla Y. Induction of apoptosis by lupeol in human epidermoid carcinoma A431 cells through regulation of mitochondrial, Akt/PKB and NFkappaB signaling pathways. Cancer Biol. Ther. 8: 1632–1639 (2009)

    Article  CAS  Google Scholar 

  23. Mertens-Talcott SU, Talcott ST, Percival SS. Low concentrations of quercetin and ellagic acid synergistically influence proliferation, cytotoxicity and apoptosis in MOLT-4 human leukemia cells. J. Nutr. 133: 2669–2674 (2003)

    CAS  Google Scholar 

  24. Dillon NL, Bally ISE, Wright CL, Hucks L, Innes DJ, Dietzgen RG. Genetic diversity of the Australian National Mango Genebank. Sci. Hortic.-Amsterdam 50: 213–226 (2013)

    Article  Google Scholar 

  25. Malik AU, Singh Z. Pre-storage application of polyamines improves shelf-life and fruit quality in mango. J. Hortic. Sci. Biotech. 80: 363–369 (2005)

    CAS  Google Scholar 

  26. Taing MW, Pierson JT, Hoang VLT, Shaw PN, Dietzgen RG, Gidley MJ, Roberts-Thomson SJ, Monteith GR. Mango fruit peel and flesh extracts affect adipogenesis in 3T3-L1 cells. Food Funct. 3: 828–836 (2012)

    Article  CAS  Google Scholar 

  27. Wilkinson AS, Flanagan BM, Pierson JT, Hewavitharana AK, Dietzgen RG, Shaw PN, Roberts-Thomson SJ, Monteith GR, Gidley MJ. Bioactivity of mango flesh and peel extracts on peroxisome proliferator-activated receptor gamma (PPARgamma) activation and MCF-7 cell proliferation: Fraction and fruit variability. J. Food Sci. 76: H11–H18 (2011)

    Article  CAS  Google Scholar 

  28. Singleton VL, Orthofer R, Lamuela-Raventós RM, Lester P. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Vol. 299, pp. 152–178. In: Methods in Enzymology. Packer L (ed). Academic Press, San Diego, CA, USA (1999)

    Google Scholar 

  29. Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well at their radical scavenging activity. Food Chem. 91: 571–577 (2005)

    Article  CAS  Google Scholar 

  30. Farmaceutyczne PT. Polish Pharmacopoeia VI. Polskie Towarzystwo Famaceutyczne. Warszawa, Poland. p. 150 (2002)

    Google Scholar 

  31. Willis RB, Allen PR. Improved method for measuring hydrolyzable tannins using potassium iodate. Analyst 123: 435–439 (1998)

    Article  CAS  Google Scholar 

  32. Curry MC, Luk NA, Kenny PA, Roberts-Thomson SJ, Monteith GR. Distinct regulation of cytoplasmic calcium signals and cell death pathways by different plasma membrane calcium ATPase isoforms in MDA-MB-231 breast cancer cells. J. Biol. Chem. 287: 28598–28608 (2012)

    Article  CAS  Google Scholar 

  33. Holiday DL, Speirs V. Choosing the right cell line for breast cancer research. Breast Cancer Res. 13: 215–222 (2011)

    Article  Google Scholar 

  34. Jänicke RU. MCF-7 breast carcinoma cells do not express caspase-3. Breast Cancer Res. Tr. 117: 219–221 (2009)

    Article  Google Scholar 

  35. Noratto GD, Bertoldi MC, Krenek K, Talcott ST, Stringheta PC, Mertens-Talcott SU. Anticarcinogenic effects of polyphenolics from mango (Mangifera indica) varieties. J. Agr. Food Chem. 58: 4104–4112 (2010)

    Article  CAS  Google Scholar 

  36. Pierson JT, Monteith GR, Roberts-Thomson SJ, Dietzgen RG, Gidley MJ, Shaw PN. Phytochemical extraction, characterisation, and comparative distribution across four mango (Mangifera indica L.) fruit varieties. Food Chem. 149: 253–263 (2014)

    Article  CAS  Google Scholar 

  37. Vergote D, Cren-Olive C, Chopin V, Toillon RA, Rolando C, Hondermarck H, Le Bourhis X. (-)-Epigallocatechin (EGC) of green tea induces apoptosis of human breast cancer cells but not of their normal counterparts. Breast Cancer Res. Tr. 76: 195–201 (2002)

    Article  CAS  Google Scholar 

  38. Hsu JD, Kao SH, Ou TT, Chen YJ, Li YJ, Wang CJ. Gallic acid induces G2/M phase arrest of breast cancer cell MCF-7 through stabilization of p27Kip1 attributed to disruption of p27Kip1/Skp2 complex. J. Agr. Food. Chem. 59: 1996–2003 (2011)

    Article  CAS  Google Scholar 

  39. Chien SY, Wu YC, Chung JG, Yang JS, Lu HF, Tsou MF, Wood WG, Kuo SJ, Chen DR. Quercetin-induced apoptosis acts through mitochondrial- and caspase-3-dependent pathways in human breast cancer MDA-MB-231 cells. Hum. Exp. Toxicol. 28: 493–503 (2009)

    Article  CAS  Google Scholar 

  40. Seeram NP, Adams LS, Hardy ML, Heber D. Total cranberry extract versus its phytochemical constituents: antiproliferative and synergistic effects against human tumor cell lines. J. Agr. Food Chem. 52: 2512–2517 (2004)

    Article  CAS  Google Scholar 

  41. Seeram NP, Adams LS, Henning SM, Niu Y, Zhang Y, Nair MG, Heber D. In vitro antiproliferative, apoptotic, and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. J. Nutr. Biochem. 16: 360–367 (2005)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Gregory Raymond Monteith.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hoang, V.L.T., Pierson, JT., Curry, M.C. et al. Polyphenolic contents and the effects of methanol extracts from mango varieties on breast cancer cells. Food Sci Biotechnol 24, 265–271 (2015). https://doi.org/10.1007/s10068-015-0035-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-015-0035-x

Keywords

Navigation