Beneficial effect of persimmon leaves and bioactive compounds on thrombosis
- 114 Downloads
The effects of ethanol extracts of persimmon leaves (EPL) and the major flavonoids catechin, epicatechin, and epicatechin gallate on blood coagulation and platelet aggregation were evaluated. Anticoagulant activities were examined by monitoring the activated partial thromboplastin time (aPTT) and the prothrombin time (PT). EPL significantly prolonged PT and aPTT. The aPTT was significantly increased in the presence of catechin, epicatechin, and epicatechin gallate, compared with controls. In order to determine the antiplatelet activity, arachidonic acid (AA), collagen, and adenosine diphosphate (ADP)-induced platelet aggregation were examined. PL and the major flavonoids significantly reduced AA-induced platelet aggregation, although they did not significantly affect ADP and collagen-induced platelet aggregation, compared with controls. EPL and the major flavonoids significantly inhibited serotonin, thromboxane A2, and soluble P-selectin generation, compared with controls. PL has potential for prevention and improvement of thrombosis by inhibiting blood coagulation and platelet activation.
Keywordspersimmon leaf anticoagulation antiplatelet
Unable to display preview. Download preview PDF.
- 3.Gopinath SCB, Shikamoto Y, Mizuno H, Kumar PK. A potent anti-coagulant RNA aptamer inhibits blood coagulation by specifically blocking the extrinsic clotting pathway. Thromb. Haemostasis 95: 767–771 (2006)Google Scholar
- 8.Jennings LK. Mechanisms of platelet activation: Need for new strategies to protect against platelet-mediated atherothrombosis. Thromb. Haemostasis 102: 248–257 (2009)Google Scholar
- 10.Konkle BA. Bleeding and thrombosis. 17th ed. pp. 369–369. In: Harrison’s Principles of Internal Medicine. Fauci AS, Braunwald E, Kasper DL. Hauser SL, Longo DL, Jameson JL, Loscalzo J (eds). McGraw Hill, New York, NY, USA (2008)Google Scholar
- 11.Badimon JJ, Weng D, Chesebro JH, Fuster V, Badimon L. Platelet deposition induced by severely damaged vessel wall is inhibited by a boroarginine synthetic peptide with antithrombin activity. Thromb. Haemostasis 71: 511–516 (1994)Google Scholar
- 17.Hu FB. Plant-based foods and prevention of cardiovascular disease: An overview. Am. J. Clin. Nutr. 78: 544S–551S (2003)Google Scholar
- 22.Miao M, Zhang X, Wang L. Persimmon leaf flavonoid induces brain ischemic tolerance in mice. Neural. Regen. Res. 8: 1376–1382 (2013)Google Scholar
- 25.World Health Organization. The top 10 causes of death. Available from: http://www.who.int/mediacentre/factsheets/fs310/en/. Accessed May 31, 2013.Google Scholar
- 28.Heemskerk JWM, Bevers EM, Lindhout T. Platelet acitivation and blood coagulation. Thromb. Haemostasis 88: 196–193 (2002)Google Scholar
- 29.Gratacap MP, Hérault JP, Viala C, Ragab A, Savi P, Herbert JM, Chap H, Plantavid M, Payrastre B. FcγRIIA requires a Gi-dependent pathway for an efficient stimulation of phosphoinositide 3-kinase, calcium mobilization, and platelet aggregation. Blood 96: 3439–3446 (2000)Google Scholar
- 35.Jin YR, Im JH, Park ES, Cho MR, Han XH, Lee JJ, Lim Y, Kim TJ, Yun YP. Antiplatelet activity of epigallocatechin gallate is mediated by the inhibition of PLCgamma2 phosphorylation, elevation of PGD2 production, and maintaining calcium-ATPase activity. J. Cardiovasc. Pharm. 51: 45–54 (2008)CrossRefGoogle Scholar