Abstract
Presence of β-amylase in ramie leaf and its anti-staling effect on starch-based foods were assessed. The ammonium sulfate fractionate (80% saturation) of the ramie leaf extracts showed a β-amylase activity, giving maltose (Glc2) as a major product, exclusively, when incubating with maltopentaose (Glc5) or soluble starch at 45°C, pH 6.0. The starch-based food product (rice cake) prepared with freeze-dried ramie leaf enzyme revealed that the linear maltooligosaccharides ranging from Glc2 to Glc6 significantly increased and the shorter branch chains (DP<15) of amylopectin increased whereas the longer branch chains (DP>16) decreased in the product. These results demonstrated that maltosyl residue was released from the non-reducing end of the longer branch chains of amylopectin by β-amylase. The ramie leaf-treatment sample significantly reduced the retrogradation rate during 48 h storage at 4°C. As an alternative plant-origin enzyme, the ramie leaf β-amylase has potential for a novel anti-staling additive.
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Nguyen, D.H.D., Tran, P.L., Ha, H.S. et al. Presence of β-amylase in ramie leaf and its anti-staling effect on rice cake. Food Sci Biotechnol 24, 37–40 (2015). https://doi.org/10.1007/s10068-015-0006-2
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DOI: https://doi.org/10.1007/s10068-015-0006-2