Abstract
A novel polysaccharide (AGP-32) from the gonad of Haliotis discus hannai Ino was isolated using a protease-assisted process and successive ion-exchange and gel-filtration chromatography. The backbone of AGP-32 was determined using hydrolysis with trifluoroacetic acid. FTIR, NMR, and methylation analysis, and periodate oxidation and Smith degradation analysis revealed that the AGP-32 backbone mainly consisted of (1→6)-linked mannose, (1→3)-linked galactose, and (1→3)-linked glucose in a proportion of 2:3:1. An in vitro cell assay indicated that AGP-32 promoted mice splenic lymphocyte proliferation by 26% at a concentration of 50 μg/mL. AGP-32 had an effect on immune protection and is a candidate for consideration as a functional food.
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Yang, Jf., Li, Yh., Zhao, J. et al. Isolation, structural characterization, and lymphopoiesis stimulant activity of a polysaccharide from the abalone gonad. Food Sci Biotechnol 24, 23–30 (2015). https://doi.org/10.1007/s10068-015-0004-4
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DOI: https://doi.org/10.1007/s10068-015-0004-4