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Enhancement of microbial oil production by alpha-linolenic acid producing Yarrowia lipolytica strains QU22 and QU137

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Abstract

Microbial oils enriched in essential polyunsaturated fatty acids can be used as nutritional complements. In order to improve the lipid yields and lipid profiles of 2 Yarrowia lipolytica strains, the effects of medium components and culture conditions were investigated using shake flasks. Under optimized conditions (cultivation for 3 days at 25oC and 150 rpm, using peptone as a nitrogen source and glucose as a carbon source), the biomass, lipid yield, and lipid content reached 6.75 g/L, 3.48 g/L, and 51.55% for Y. lipolytica QU22, and 6.85 g/L, 3.52 g/L, and 51.38% for Y. lipolytica QU137, respectively. Using a shaking speed of 150 rpm, unsaturated fatty acids constituted 87.96% (QU22) and 87.08% (QU137) of the total lipids produced by these strains, with 9.52 and 7.86% of alpha-linolenic acid, respectively. The strains are suitable candidates for fermentation processes involving essential polyunsaturated fatty acid (PUFA) production.

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Authors and Affiliations

  1. Department of Food Science and Technology, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, 97105-900, Brazil

    Paula Mattanna, Ana Paula Gusso & Neila S. P. S. Richards

  2. Department of Microbiology, Immunology and Parasitology, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, 90050-170, Brazil

    Priscila Dallé da Rosa & Patrícia Valente

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  1. Paula Mattanna
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  2. Priscila Dallé da Rosa
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Correspondence to Paula Mattanna.

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Mattanna, P., Dallé da Rosa, P., Gusso, A.P. et al. Enhancement of microbial oil production by alpha-linolenic acid producing Yarrowia lipolytica strains QU22 and QU137. Food Sci Biotechnol 23, 1929–1934 (2014). https://doi.org/10.1007/s10068-014-0263-5

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  • DOI: https://doi.org/10.1007/s10068-014-0263-5

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