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Characteristics and growth inhibition of isolated bacteriophages for Enterococcus faecalis

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Abstract

Enterococcus faecalis has been used as a starter for fermented food and probiotics, but it can produce biogenic amine in foods and also cause infectious diseases in humans. The antibiotic resistance acquired by E. faecalis restricts antibiotic prescriptions in clinical settings. Bacteriophages EFP4 and EFP7 for E. faecalis, isolated from soil samples, impeded E. faecalis growth. Morphological analysis showed that bacteriophages EFP4 and EFP7 belonged to the Siphoviridae. Bacteriophages EFP4 and EFP7 were susceptible to temperatures above 70°C; however, stability was slightly reduced to 2–3 log PFU/mL after 30 min in 70% ethanol. Two bacteriophages did not suffer phage resistant in E. faecalis for 24 h. Therefore, bacteriophages EFP4 and EFP7, after more study, are candidates for use in biocontrol and controlled fermentation of E. faecalis through food applications.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Gachon University, Seongnam, Gyeonggi, 461-701, Korea

    Young-Duck Lee & Jong-Hyun Park

  2. Food Science and Engineering, School of Convergence Bioscience and Technology, Seowon University, Cheongju, Chungbuk, 361-742, Korea

    Young-Duck Lee & Honam Chun

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  1. Young-Duck Lee
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  2. Honam Chun
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  3. Jong-Hyun Park
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Correspondence to Jong-Hyun Park.

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Lee, YD., Chun, H. & Park, JH. Characteristics and growth inhibition of isolated bacteriophages for Enterococcus faecalis . Food Sci Biotechnol 23, 1357–1361 (2014). https://doi.org/10.1007/s10068-014-0186-1

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  • DOI: https://doi.org/10.1007/s10068-014-0186-1

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