Skip to main content
SpringerLink
Log in

Modification of methods for detection of Escherichia coli O157:H7 on produce

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

To establish the best detection method for Escherichia coli O157:H7 on produce, the sensitivity, specificity, and recovery values of 3 selective media [sorbitol MacConkey agar containing cefixime and tellurite (CTSMAC), sorbitol MacConkey agar containing cefixime, tellurite, and 5-bromo-4-chloro-3-indoxyl-β-glucuronide (CTB-SMAC), and CHROMagar O157 were compared. Sample preparation methods (pummeling, sonication, and hand-shaking) were compared to determine the optimum procedure for qualitative analysis from cherry tomato. The selectivity and recovery rates of stressed cell of the 3 tested media were in the order of CHROMagar O157>CTBSMAC>CT-SMAC. The recovery rates of hand-shaken tomatoes (10/10) were higher than rates of sonicated (8/10) and pummeled tomatoes (6/10) for inoculated cherry tomatoes in modified E. coli (mEC) broth enriched with novobicin. CHROMagar O157 was the optimum agar medium for detection of E. coli O157:H7. Cherry tomatoes should be treated with hand-shaking to enhance recovery for qualitative analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Griffin PM, Tauxe RV. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli and the associated hemolytic uremic syndrome. Epidemiol. Rev. 13: 60–98 (1991)

    CAS  Google Scholar 

  2. Karmalli MA. Infection by verotoxin-producing Escherichia coli. Clin. Microbiol. Rev. 2: 15–38 (1989)

    Google Scholar 

  3. Centers for Disease Control. Update on multi-state outbreak of E. coli O157:H7 infections from fresh spinach, October 6, 2006. Available from: http://www.cdc.gov/ecoli/2006/september/updates/100606.html. Accessed Feb. 20, 2010.

    Google Scholar 

  4. Itoh Y, Sugita-Konishi Y, Kasuga F, Iwaki M, Hara-Kuda Y, Saito N, Noguchi Y, Konuma H, Kumagai S. Enterohemorrhagic Escherichia coli O157:H7 present in radish sprouts. Appl. Environ. Microbiol. 64: 1532–1535 (1998)

    CAS  Google Scholar 

  5. Michino H, Araki K, Minami S, Takaya S, Sakai N, Miyazaki M, Ono A, Yanagawa H. Massive outbreak of Escherichia coli O157:H7 infection in school children in Sakai City, Japan, associated with consumption of white radish sprout. Am. J. Epidemiol. 150: 787–796 (1999)

    Article  CAS  Google Scholar 

  6. Onoue Y, Konuma H, Nakagawa H, Hara-Kudo Y, Fujita T, Kumagai S. Collaborative evaluation of detection methods for Escherichia coli O157:H7 from radish sprouts and ground beef. Int. J. Food Microbiol. 46: 27–36 (1999)

    Article  CAS  Google Scholar 

  7. Weagant SD, Feng PCH. Comparative analysis of a modified rapid presence/absence test and the standard MPN method for detecting Escherichia coli in orange juice. Food Microbiol. 19: 111–115 (2002)

    Article  Google Scholar 

  8. Weagant SD, Jinneman KC, Yoshitomi KJ, Zapata R, Fedio WM. Optimization and evaluation of modified enrichment procedure combined with immunomagnetic separation for detection of E. coli O157:H7 from artificially contaminated alfalfa sprouts. Int. J. Food Microbiol. 149: 209–217 (2011)

    Article  Google Scholar 

  9. Weagant SD, Bound AJ. Evaluation of techniques for enrichment and isolation of Eschericha coli O157:H7 from artificially contaminated sprouts. Int. J. Food Microbiol. 71: 87–92 (2001)

    Article  CAS  Google Scholar 

  10. March SB, Ratnam S. Sorbitol-MacConkey medium for detection of Escherichia coli O157:H7 associated with hemorrhagic colitis. J. Clin. Microbiol. 23: 869–872 (1986)

    CAS  Google Scholar 

  11. Fujisawa T, Sata S, Aikawa K, Takahashi T, Yamai S, Shimada T. Modification of sorbitol MacConkey medium containing cefixime and tellurite for isolation of Eshcherichia coli O157:H7 from radish sprouts. Appl. Environ. Microbiol. 66: 3117–3118 (2000)

    Article  CAS  Google Scholar 

  12. Fujisawa T, Sata S, Aikawa K, Takahashi T, Yamai S, Shimada T. Evaluation of sorbitol-salicin MacConkey medium containing cefixime and tellurite (CT-SSMAC medium) for isolation of Escherichia coli O157:H7 from raw vegetables. Int. J. Food Microbiol. 74: 161–163 (2002)

    Article  CAS  Google Scholar 

  13. Okrend AJG, Rose BE, Lattuada CP. Use of 5-bromo-4-chloro-3-indoxyl-β-d-glucuronide in MacConkey sorbitol agar to aid in the isolation of E. coli O157:H7 from ground beef. J. Food Prot. 53: 941–943 (1990)

    CAS  Google Scholar 

  14. Zadik PM, Chapman PA, Siddons CA. Use of tellurite for the selection of verocytoxigenic E. coli O157. J. Med. Microbiol. 39: 155–158 (1993)

    Article  CAS  Google Scholar 

  15. Blackburn CW, McCarthy JD. Modifications to methods for the enumeration and detection of injured Escherichia coli O157:H7 in foods. Int. J. Food Microbiol. 55: 285–290 (2000)

    Article  CAS  Google Scholar 

  16. Conner DE, Hall GS. Efficacy of selected media for recovery of Escherichia coli O157:H7 from frozen chicken meat containing sodium chloride, sodium lactate or polyphosphate. Food Microbiol. 11: 337–344 (1994)

    Article  CAS  Google Scholar 

  17. McCarthy J, Holbrook R, Stephens PJ. An improved direct plate method for the enumeration of stressed Escherichia coli O157:H7 from food. J. Food Prot. 61: 1093–1097 (1998)

    CAS  Google Scholar 

  18. Taormina PJ, Clavero MRS, Beuchat LR. Comparison of selective agar media and enrichment broths for recovering heat-stressed E. coli O157:H7 from ground beef. Food Microbiol. 15: 631–638 (1998)

    Article  Google Scholar 

  19. Manafi M, Kremsmaier B. Comparative evaluation of different chromogenic/fluorogenic media for detecting Escherichia coli O157:H7 in food. Int. J. Food Microbiol. 71: 257–262 (2001)

    Article  CAS  Google Scholar 

  20. Hepbrun NF, MacRae M, Johnston M, Mooney J, Ogden ID. Optimizing enrichment conditions for the isolation of Escherichia coli O157 in soils by immunomagnetic separation. Lett. Appl. Microbiol. 34: 365–369 (2002)

    Article  Google Scholar 

  21. Kim SR, Yoon Y, Kim WI, Park KH, Yun HJ, Chung DH, Yun JC, Ryu KY. Comparison of sample preparation methods for the recovery of food-borne pathogens from fresh produce. J. Food Prot. 75: 1213–1218 (2012)

    Article  Google Scholar 

  22. Burnett AB, Beuchat LR. Comparison of sample preparation methods for recovering Salmonella from raw fruits, vegetables, and herbs. J. Food Prot. 64: 1459–1465 (2001)

    CAS  Google Scholar 

  23. Park SH, Ryu S, Kang DH. Improved selective and differential medium for isolation of Escherichia coli O157:H7. J. Clin. Microbiol. 49: 405–408 (2011)

    Article  Google Scholar 

  24. Jasson V, Uyttendaele M, Rajkovic A, Debevere J. Establishment of procedures provoking sub-lethal injury of Listeria monocytogenes, Campylobacter jejuni and Escherichia coli O157 to serve method performance testing. Int. J. Food Microbiol. 118: 241–249 (2007)

    Article  CAS  Google Scholar 

  25. Food and Drug Administration. Analysis and evaluation of preventive control measures for the control and reduction/elimination of microbial hazards on fresh and fresh-cut produce. Available from: http://www.fda.gov/Food/ScienceResearch/ResearchAreas/SafePracticesforFoodProcesses/ucm090977.html. Accessed Jun. 5, 2009.

    Google Scholar 

  26. Fujisawa T, Mori M. Species of coliform bacteria isolated from market samples of raw vegetables. J. Antibact. Antifungal Agent. Jpn. 20: 251–253 (1992)

    Google Scholar 

  27. Dehghani MH. Effectiveness of ultrasound on the destruction of E. coli. Am. J. Environ. Sci. 1: 187–189 (2005)

    Article  Google Scholar 

  28. Charkowski, AO, Barak, JD, Sarreal, CZ, Mandrell, RE. Differences in growth of Salmonella enterica and Escherichia coli O157:H7 on alfalfa sprouts. Appl. Environ. Microbiol. 68: 3114–3120 (2002)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Microbial Safety Division, Department of Crop Life Safety, National Academy of Agricultural Science, Rural Development Administration, Suwon, Gyeonggi, 441-707, Korea

    Se-Ri Kim, Min-Kyoung Seo, Won-Il Kim, Jong Chul Yun & Byung-Seok Kim

  2. Department of Food and Nutrition, Sookmyung Women’s University, Seoul, 140-742, Korea

    Yohan Yoon

  3. Division of Applied Life Science, Graduate School of Gyeongsang National University, Jinju, Gyeongnam, 660-701, Korea

    Won-Bo Shim & Duck Hwa Chung

  4. R&D Evaluation Division, Research Policy Bureau, Rural Development Administration, Suwon, Gyeonggi, 441-707, Korea

    Kyoung Yul Ryu

Authors
  1. Se-Ri Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yohan Yoon
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Min-Kyoung Seo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Won-Il Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Won-Bo Shim
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Duck Hwa Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jong Chul Yun
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Kyoung Yul Ryu
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Byung-Seok Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Byung-Seok Kim.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kim, SR., Yoon, Y., Seo, MK. et al. Modification of methods for detection of Escherichia coli O157:H7 on produce. Food Sci Biotechnol 23, 1349–1356 (2014). https://doi.org/10.1007/s10068-014-0185-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-014-0185-2

Keywords

Search

Navigation