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Anticancer activity of 28-Oxoallobetulin on HT-29 human colon cancer cells

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Abstract

Many successful therapeutic drug, including several anticancer agents, have been isolated for natural products. In a recent screen of methanolic extracts from several natural products, we isolated an active compound, from the methanolic extract of persimmon calyx, which promoted cytotoxicity and apoptosis in HT-29 human colon cancer cells. This compound was identified as 28-oxoallobetulin, a prototypical compound, by UV-MS analysis and various NMR spectroscopic methods. The highly cytotoxic effects of 28-oxoallobetulin on HT-29 cells were demonstrated using MTT reduction, LDH release, and colony formation assays. Furthermore, we were able to detect apoptotic bodies in HT-29 cells by Hoechst staining as well as flow cytometric analysis for the presence of sub-G1 DNA peaks, indicative of apoptotic cells. Finally apoptosis in HT-29 cells following 28-oxoallobetulin treatment was also confirmed by the presence of activated caspase-3 and specific proteolytic cleavage of poly (ADP-ribose) polymerase.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Kyungnam University, Masan, Gyeongnam, 631-701, Korea

    Jeung-Min Lee, Dahee Koo & Hae-Ryong Park

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  1. Jeung-Min Lee
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  2. Dahee Koo
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  3. Hae-Ryong Park
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Correspondence to Hae-Ryong Park.

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Lee, JM., Koo, D. & Park, HR. Anticancer activity of 28-Oxoallobetulin on HT-29 human colon cancer cells. Food Sci Biotechnol 23, 1321–1325 (2014). https://doi.org/10.1007/s10068-014-0181-6

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  • DOI: https://doi.org/10.1007/s10068-014-0181-6

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