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Optimization of omega-3 enriched-diacylglycerol production by enzymatic esterification using a response surface methodology

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Abstract

Incorporation of an eicosapentaenoic acid/docosahexaenoic acid (EPA/DHA) moiety into diacylglycerol (DAG) oil using lipase-catalyzed esterification was optimized using an ethyl ester form of EPA/DHA. A response surface methodology (RSM) was used to optimize reaction parameters (time, temperature, and substrate mole ratio) for incorporation of DHA and EPA into DAG oil. Predictive models for DHA+EPA contents of DAG and the amount of DAG produced after esterification were adequate and reproducible. DHA+EPA contents of DAG significantly increased with reaction time and substrate mole ratio (p<0.05). In contrast, the reaction temperature negatively affected the amount of DAG after esterification. Synthesis of DHA+EPA-enriched DAG was optimized for a maximum DAG content with the highest DHA+EPA content, in which 630.0 mg of DAG containing 34.8% DHA and EPA was predicted using the RSM model. The optimal reaction conditions were predicted at 20.6 h, 57.9 and a DHA/EPAenriched ethyl ester: DAG oil ratio of 2.5:1.

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Authors and Affiliations

  1. Department of Food Science and Technology, Chungnam National University, Daejeon, 305-764, Korea

    Jin-Feng Qi, Xiang-Yu Wang & Hua Zhang

  2. Department of Food and Nutrition, Daegu University, Gyeongsan, Gyeongbuk, 712-714, Korea

    Jeung Hee Lee

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  1. Jin-Feng Qi
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  2. Xiang-Yu Wang
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Correspondence to Jeung Hee Lee.

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Qi, JF., Wang, XY., Zhang, H. et al. Optimization of omega-3 enriched-diacylglycerol production by enzymatic esterification using a response surface methodology. Food Sci Biotechnol 23, 1129–1136 (2014). https://doi.org/10.1007/s10068-014-0154-9

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  • DOI: https://doi.org/10.1007/s10068-014-0154-9

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