Food Science and Biotechnology

, Volume 23, Issue 4, pp 1067–1072 | Cite as

Improving mineral availability in soymilk by dephosphorylation of phytic acid using an alkaline phytase from Bacillus amyloliquefaciens DS11

  • Da-Ae Kwon
  • Sunghoon Park
  • Dodan Kwon
  • Kyung-Heon Kim
  • Byung-Chul Oh
  • Joong-Hyuck AuhEmail author
Research Article


To enhance mineral availability in soymilk, the alkaline phytase from Bacillus amyloliquefaciens DS11 was applied to degrade the phytic acid in soymilk, and resulting effects on mineral profiles were assessed. Nearly 60% of phytic acid was degraded within 25 min by treatment with 0.090 unit/mg of phytate, whereas most phytic acid was removed in 125 min using 0.18 unit/mg of phytate. Compared to a control, the free calcium, magnesium, phosphorus, and iron contents significantly (p<0.05) increased by 1.9, 1.8, 4.0, and 4.0×, respectively, after 125 min of reaction using 0.18 unit/mg of phytate. Free minerals were released from the phytate complex by enzymatic dephosphorylation, resulting in enhancement of mineral availability in soymilk. Alkaline phytase DS11 has a great potential for enhancing the mineral availability in neutral pH phytate-rich foods, like soymilk.


soymilk alkaline phytase phytic acid mineral 


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Copyright information

© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Da-Ae Kwon
    • 1
  • Sunghoon Park
    • 2
  • Dodan Kwon
    • 1
  • Kyung-Heon Kim
    • 1
  • Byung-Chul Oh
    • 2
  • Joong-Hyuck Auh
    • 1
    Email author
  1. 1.Department of Food Science & TechnologyChung-Ang UniversityAnseong, GyeonggiKorea
  2. 2.Lee Gil Ya Cancer and Diabetes InstituteGachon University of Medicine & ScienceIncheonKorea

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