Skip to main content

Protective effect of polyamine extract of salt stressed and sprouted soybean seeds against ethanol-induced gastric ulcer in rats

Abstract

The anti-ulcer activity of polyamine extract (PAE) of salt stressed and sprouted soybean seeds against ethanol-induced gastric damage was investigated in a rat model. The contents of the polyamines putrescine (20.11%), spermidine (9.46%), and spermine (2.79%) in PAE were determined using HPLC. The anti-ulcer activity of the extract was compared with the effects of the reference drug omeprazole. Pre- and post-administration of PAE at doses of 10+10, 20, and 20+20mg/kg of body weight in conjunction with ethanol administration significantly protected ethanolinduced gastric damage. The levels of superoxide dismutase and glutathione in stomach tissues were significantly changed, when compared with an ethanol control group. Polyamine extract exhibited high protective effect against ulcer lesions and could be used to develop new anti-ulcer drugs.

This is a preview of subscription content, access via your institution.

Refrerences

  1. 1.

    Steiner N, Santa-Catarina C, Silveira V, Floh EIS, Guerra MP. Polyamine effects on growth and endogenous hormones levels in Araucaria angustifolia embryogenic cultures. Plant Cell Tiss. Org. Cult. 89: 55–62 (2007)

    CAS  Article  Google Scholar 

  2. 2.

    Kaur-Sawhney R, Tiburcio AF, Altabella T, Galston AW. Polyamines in plants: An overview. J. Cell Mol. Biol. 2: 1–12 (2003)

    Google Scholar 

  3. 3.

    Brzozowski T, Drozdowicz D, Majka J, Polonczyk-Pytko J, Konturek SJ. Role of polyamines in gastroprotection induced by epidermal growth factor. J. Physiol. Pharmacol. 42: 181–193 (1991)

    CAS  Google Scholar 

  4. 4.

    Konturek SJ, Brzozowski T, Kontureck PK, Majka J, Dembinski A. Role of salivary glands and epidermal growth factor (EGF) in gastric secretion and mucosal integrity in rats exposed to stress. Regul. Pept. 32: 203–215 (1991)

    CAS  Article  Google Scholar 

  5. 5.

    Fujisawa S, Kadoma Y. Kinetic evaluation of polyamines as radical scavengers. Anticancer Res. 25: 965–969 (2005)

    CAS  Google Scholar 

  6. 6.

    Soda K, Kano Y, Nakamura T, Kasono K, Kawakami M, Konishi F. Spermine, a natural polyamine, suppresses LFA-1 expression on human lymphocyte. J. Immunol. 175: 237–245 (2005)

    CAS  Article  Google Scholar 

  7. 7.

    Soda K. Polyamine intake, dietary pattern, and cardiovascular disease. Med. Hypotheses 75: 299–301 (2010)

    CAS  Article  Google Scholar 

  8. 8.

    Ramot Y, Pietila M, Giuliani G, Rinaldi F, Alhonen L, Paus R. Polyamines and hair: A couple in search of perfection. Exp. Dermatol. 19: 784–790 (2010)

    CAS  Article  Google Scholar 

  9. 9.

    Amal BK, Aurang Z, Nizakat B, Shahid AK, Mohammad SK. Influence of germination techniques on phytic acid and polyphenols content of chickpea (Cicer arietinum L.) sprouts. Food Chem. 104: 1074–1079 (2007)

    Article  Google Scholar 

  10. 10.

    Lintschinger J, Fuchs N, Moser H, Jager R, Hlebeina T, Markolin G, Gossler W. Uptake of various trace elements during germination of wheat, buckwheat and quinoa. Plant Foods Hum. Nutr. 50: 223–237 (1997)

    CAS  Article  Google Scholar 

  11. 11.

    Rubio LA, Muzquiz M, Burbano C, Cuadrado C, Pedrosa MM, High apparent leal digestibility of amino acids in raw and germinated faba bean (Vicia faba) and chickpea (Cicer arietinum) based diets for rats. J. Sci. Food Agr. 82: 1710–1717 (2002)

    CAS  Article  Google Scholar 

  12. 12.

    Munns R, Termatt A. Whole plant response to salinity. pp. 143-160. In: Plant Growth, Drought, and Salinity. Turner NC, Passioura I (eds). CSIRO Publishing, Canberra, Australia (1986)

    Google Scholar 

  13. 13.

    Sannazzaro AI, Echeverria M, Alberto EO, Ruiz OA, Menendez AB. Modulation of polyamine balance in Lotus glaber by salinity and arbuscular mycorrhiza. Plant Physiol. Biochem. 45: 39–46 (2007)

    CAS  Article  Google Scholar 

  14. 14.

    Campestre MP, Bordenave CD, Origone AC, Menendez AB, Ruiz OA, Rodriguez AA, Maiale SJ. Polyamine catabolism is involved in response to salt stress in soybean hypocotyls. J. Plant Physiol. 168: 1234–1240 (2011)

    CAS  Article  Google Scholar 

  15. 15.

    Wang LC. Polyamines in soybeans. Plant Physiol. 50: 152–156 (1972)

    CAS  Article  Google Scholar 

  16. 16.

    Tan PV, Nditafon GN, Yewah MP, Dimo T, Ayafor JF. Eremomastax speciosa: Effects of leaf aqueous extract on ulcer formation and gastric secretion in rats. J. Ethnopharmacol. 54: 139–142 (1996)

    CAS  Article  Google Scholar 

  17. 17.

    Nishimura K, Shiina R, Kashiwagi K, Igarashi K. Decrease of polyamines with ageing and their ingestion from food and drink. J. Biochem. 139: 81–90 (2006)

    CAS  Article  Google Scholar 

  18. 18.

    Okamoto A, Sugi E, Koizumi Y, Yanagida F, Udaka S. Polyamine content of ordinary foodstuffs and various fermented foods. Biosci. Biotech. Bioch. 61: 1582–1584 (1997)

    CAS  Article  Google Scholar 

  19. 19.

    Kalac P, Krizek M, Pelikanova T, Langova M, Veskrna O. Contents of polyamines in selected foods. Food Chem. 90: 561–564 (2005)

    CAS  Article  Google Scholar 

  20. 20.

    Righetti L, Tassoni A, Bagni N. Polyamines content in plant derived food: A comparison between soybean and Jerusalem artichoke. Food Chem. 111: 852–856 (2008)

    CAS  Article  Google Scholar 

  21. 21.

    Choi E, Hwang H, Kim I, Nam T. Protective effects of a polysaccharide from Hizikia fusiformis against ethanol toxicity in rats. Food Chem. Toxicol. 47: 134–139 (2009)

    CAS  Article  Google Scholar 

  22. 22.

    Ineu RP, Pereira ME, Aschner M, Nogueira CW, Zeni G, Rocha JBT. Diphenyl diselenide reverses gastric lesions in rats: involvement of oxidative stress. Food Chem. Toxicol. 46: 3023–3029 (2008)

    CAS  Article  Google Scholar 

  23. 23.

    Hirokawa M, Miura S, Yoshida H, Kurose I, Shigematsu T, Hokari R, Higuchi H, Watanabe N, Yokoyama Y, Kimura H, Kato S, Ishii H. Oxidative stress and mitochondrial damage precedes gastric mucosal cell death induced by ethanol administration. Alcohol Clin. Exp. Res. 22: 111S–114S (1998)

    CAS  Article  Google Scholar 

  24. 24.

    Singh S, Khajuria A, Taneja SC, Khajuria RK, Singh J, Johri RK, Qazi GN. The gastric ulcer protective effect of boswellic acids, a leukotriene inhibitor from Boswellia serrata, in rats. Phytomedicine 15: 408–415 (2008)

    CAS  Article  Google Scholar 

  25. 25.

    Devi RS, Narayan S, Vani G, Devi CSS. Gastroprotective effect of Terminalia arjuna bark on diclofenac sodium induced gastric ulcer. Chem. Biol. Interact. 67: 71–83 (2007)

    Article  Google Scholar 

  26. 26.

    Mizui T, Doteuchi M. Effect of polyamines on acidified ethanolinduced gastric lesions in rats. Jpn. J. Pharmacol. 33: 939–945 (1983)

    CAS  Article  Google Scholar 

  27. 27.

    Arun M, Asha VV. Gastroprotective effect of Dodonaea viscosa on various experimental ulcer models. J Ethnopharmacol. 118: 460–465 (2008)

    CAS  Article  Google Scholar 

  28. 28.

    Liu Y, Tian X, Gou L, Fu X, Li S, Lan N, Yin X. Protective effect of l-citrulline against ethanol-induced gastric ulcer in rats. Environ. Toxicol. Pharmacol. 3: 280–287 (2012)

    Article  Google Scholar 

  29. 29.

    Alimi H, Hfaiedh N, Bouoni Z, Sakly M, Rhouma KB. Evaluation of antioxidant and antiulcerogenic activities of Opuntia ficus indica f. inermis flowers extract in rats. Environ. Toxicol. Pharmacol. 32: 406–416 (2011)

    CAS  Article  Google Scholar 

  30. 30.

    Sehirli O, Tatlidede E, Yuksel M, Erzik C, Cetinel S, Yegen BC, Sener G. Antioxidant effect of alpha-lipoic acid against ethanolinduced gastric mucosal erosion in rats. Pharmacology 81: 173–180 (2008)

    CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Sun Chul Kang.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Sowndhararajan, K., Paul, S., Kwon, G.S. et al. Protective effect of polyamine extract of salt stressed and sprouted soybean seeds against ethanol-induced gastric ulcer in rats. Food Sci Biotechnol 23, 711–716 (2014). https://doi.org/10.1007/s10068-014-0096-2

Download citation

Keywords

  • soybean
  • polyamine
  • sprouted
  • anti-ulcer
  • gastroprotective