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Selective cytotoxicity of Saururus chinensis in glucose-deprived HT-29 human colon cancer cells

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Abstract

In this study, the selective cytotoxic activity of methanolic extracts of Saururus chinensis (SCE) was examined on glucose-deprived HT-29 cells. The effects of SCE on 2-deoxy-glucose (2DG) or glucose-free stressed HT-29 cells were evaluated through the MTT reduction assay, morphological observations, and the colony formation assay, in which SCE (5, 10 μg/mL) was found to be highly toxic to HT-29 cells only during glucose-deprived conditions. In addition, the mechanism of selective cytotoxic effects on SCE was assessed by Western blot analysis; SCE suppressed the accumulation of GRP78. Furthermore, apoptotic effects of SCE on glucose-deprived HT-29 cells were identified by Hoechst 33342 staining and flow cytometric analysis. In conclusion, SCE induced apoptosis in HT-29 cells under glucose-deprived conditions by down-regulation of GRP78. Cytotoxic effects on HT-29 cells were only observed during glucose deprivation, suggesting that SCE may be a target for novel therapeutic agents for treating colon cancer under glucose-deprived stress.

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References

  1. Acker T, Plate KH. A role for hypoxia and hypoxia-inducible transcription factors in tumor physiology. J. Mol. Med. 80: 562–575 (2002)

    Article  CAS  Google Scholar 

  2. Hiroyasu Y. Solid tumor physiology and hypoxia-induced chemo/radio-resistance: Novel strategy for cancer therapy: Nitric oxide donor as a therapeutic enhancer. Nitric Oxide 19: 205–216 (2008)

    Article  Google Scholar 

  3. Hwang JH, Kim JY, Cha MR, Park HR. Effect of methanolic extract from silkworm droppings on proliferation and caspase activity in HT-29 human colon cancer cells. J. Med. Food. 10: 467–472 (2007)

    Article  CAS  Google Scholar 

  4. Semenza GL. Angiogenesis in ischemic and neoplastic disorders. Annu. Rev. Med. 54: 17–28 (2003)

    Article  CAS  Google Scholar 

  5. Harding HP, Calfor M, Urano F, Novoa I, Ron D. Transcriptional and translational control in the mammalian unfolded protein response. Annu. Rev. Cell Dev. Biol. 18: 575–599 (2002)

    Article  CAS  Google Scholar 

  6. Park HR, Tomida A, Sato S, Tsukumo Y, Yun J, Yamori T, Hayakawa Y, Tsuruo T, Shin-ya K. Effect on tumor cells of blocking survival response to glucose deprivation. J. Natl. Cancer Inst. 96: 1300–1310 (2004)

    Article  CAS  Google Scholar 

  7. Hwang JH, Kim JY, Cha MR, Ryoo IJ, Choo SJ, Cho SM, Tsukumo Y, Tomida A, Shin-ya K, Hwang YI, Yoo ID, Park HR. Etoposideresistant HT-29 human colon carcinoma cells during glucose deprivation are sensitive to piericidin A, a GRP78 down-regulator. J. Cell. Physiol. 215: 243–250 (2008)

    Article  CAS  Google Scholar 

  8. Kim R, Emi M, Tanabe K, Murakami S. Role of the unfolded protein response in cell death. Apoptosis 11: 5–13 (2006)

    Article  CAS  Google Scholar 

  9. Park HR, Ryoo IJ, Choo SJ, Hwang JH, Kim JY, Cha MR, Shin-ya K, Yoo ID. Glucose-deprived HT-29 human colon carcinoma cells are sensitive to verrucosidin as a GRP78 down-regulator. Toxicology 229: 253–261 (2007)

    Article  CAS  Google Scholar 

  10. Philip LM, Ekaterina VBM, Steve FA. Expression of the proangiogenic factors vascular endothelial growth factor and interleukin-8/CXCL8 by human breast carcinomas is responsive to nutrient deprivation and endoplasmic reticulum stress. Mol. Cancer 3: 1–12 (2004)

    Article  Google Scholar 

  11. Suzuki H, Tomida A, Tsuruo T. Dephosphorylated hypoxiainducible factor 1α as a mediator of p53-dependent apoptosis during hypoxia. Oncogene 20: 5779–5788 (2001)

    Article  CAS  Google Scholar 

  12. Park HR, Furihata K, Hayakawa Y, Shin-ya K. Versipelostatin, a novel GRP78/Bip molecular chaperone down-regulator of microbial origin. Tetrahedron Lett. 43: 6941–6945 (2002)

    Article  CAS  Google Scholar 

  13. Gazit G, Hung G, Chen X, Anderson WF, Lee SA. Use of the glucose starvation-inducible glucose-regulated protein 78 promoter in suicide gene therapy of murine fibrosarcoma. Cancer Res. 59: 3100–3106 (1999)

    CAS  Google Scholar 

  14. Lee EJ, Nichols P, Spicer D, Groshen S, Yu MC, Lee AS. GRP78 as a novel predictor of responsiveness to chemotherapy in breast cancer. Cancer Res. 66: 7849–7853 (2006)

    Article  CAS  Google Scholar 

  15. Ryoo IJ, Park HR, Choo SJ, Hwang JH, Park YM, Bae KH, Shin-ya K, Yoo ID. Selective cytotoxic activity of Valinomycin against HT-29 human colon carcinoma cells via down-regulation of GRP78. Biol. Pharm. Bull. 29: 817–820 (2006)

    Article  CAS  Google Scholar 

  16. Choo SJ, Park HR, Ryoo IJ, Kim JP, Yun BS, Kim CJ, Shin-ya K, Yoo ID. Deoxyverrucosidin, a novel GRP78/BiP down-regulator, produced by Penicillium sp. J. Antibiot. 58: 210–213 (2005)

    Article  CAS  Google Scholar 

  17. Ogiso Y, Tomida A, Lei S, Omura S, Tsuruo T, Proteasome inhibition circumvents solid tumor resistance to topoisomerase II-directed drugs. Cancer Res. 60: 2429–2434 (2000)

    CAS  Google Scholar 

  18. Ogiso Y, Tomida A, Tsuruo T. Nuclear localization of proteasomes participates in stress-inducible resistance of solid tumor cells to topoisomerase II-directed drugs. Cancer Res. 62: 5008–5012 (2002)

    CAS  Google Scholar 

  19. Suzuki H, Tomida A, Tsuruo T. A novel mutant from apoptosisresistant colon cancer HT-29 cells showing hyper-apoptotic response to hypoxia, low glucose and cisplatin. Jpn. J. Cancer Res. 89: 1169–1178 (1998)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Kyungnam University, Gyeongnam, 631-701, Korea

    Eun-Soon Son, Jeong-Un Choi, Kyung-Jin Lee & Hae-Ryong Park

  2. International Tuberculosis Research Center, Changwon, Gyeongnam, 631-710, Korea

    Eun-Soon Son

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  1. Eun-Soon Son
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  2. Jeong-Un Choi
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Correspondence to Hae-Ryong Park.

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Son, ES., Choi, JU., Lee, KJ. et al. Selective cytotoxicity of Saururus chinensis in glucose-deprived HT-29 human colon cancer cells. Food Sci Biotechnol 23, 609–613 (2014). https://doi.org/10.1007/s10068-014-0083-7

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  • DOI: https://doi.org/10.1007/s10068-014-0083-7

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