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Protective effects of dehydroglyasperin c against carbon tetrachloride-induced liver damage in mice

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Abstract

Dehydroglyasperin C (DGC) isolated from licorice has been shown to exhibit antioxidant activity as well as induce phase 2 detoxifying enzymes in mouse hepatoma cells. This study investigated whether or not DGC exerts hepatoprotective effects through modulation of phase 1 and 2 detoxifying enzymes. ICR mice were divided into five groups with 10 mice per group: (1) negative control, (2) positive control injected with carbon tetrachloride (CCl4, 0.6 mL/kg BW) alone, (3) ICR mice injected with DGC (5 mg/kg BW) alone, (4) ICR mice injected with DGC followed by CCl4, and (5) ICR mice injected with DGC and CCl4 simultaneously. Mice were adapted for 1 week, followed by injection with DGC on day 7, CCl4 on day 8, and sacrifice on day 9 of the experiment. Treatment with DGC induced NQO1 activity in kidney only, but not in the other tissues of mice. Compared to mice injected with CCl4 alone, mice simultaneously injected with both DGC and CCl4 showed reduced lipid droplet formation in liver tissue, as assessed by histological examination. Further, DGC demonstrated a slight protective effect against centrilobular injury caused by CCl4 injection, perhaps through suppression of CYP2E1 expression. In conclusion, DGC possesses hepatoprotective effects against CCl4-induced injury.

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Authors and Affiliations

  1. School of Applied Biosciences and Food Science and Biotechnology, Kyungpook National University, Daegu, 702-701, Korea

    Ji Yeon Seo & Jong-Sang Kim

  2. Department of Pharmacology, College of Medicine, Yeungnam University, Daegu, 705-717, Korea

    Jung Hwa Han

  3. Department of Biotechnology, the Graduate School of Biotechnology, Korea University, Seoul, 136-713, Korea

    Yeon Ji Kim

  4. Department of Food Science and Nutrition, Hallym University, Gangwon, 200-702, Korea

    Soon Sung Lim

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  1. Ji Yeon Seo
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  2. Jung Hwa Han
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  4. Soon Sung Lim
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  5. Jong-Sang Kim
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Correspondence to Jong-Sang Kim.

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Seo, J.Y., Han, J.H., Kim, Y.J. et al. Protective effects of dehydroglyasperin c against carbon tetrachloride-induced liver damage in mice. Food Sci Biotechnol 23, 547–553 (2014). https://doi.org/10.1007/s10068-014-0075-7

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  • DOI: https://doi.org/10.1007/s10068-014-0075-7

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