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Fractionation of nitrogenous compounds from water extracts of doenjang and their physiological activities

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Abstract

Nitrogenous compounds were separated from a water extract of doenjang (WED), and the distribution of their molecular weights, and anti-inflammatory and angiotensin-I converting enzyme (ACE) inhibitory activities were investigated. WED was fractionated by ultrafiltration into 5 individual groups. The nitrogenous compound content in the 5 fractions were 1,062.1, 34.5, 24.2, and 29.9 mg% for WED I, WED II, WED III, and WED V, respectively. ACE inhibitory activity was widely observed in all the fractions, which suggest that many ACE inhibitory peptides with various molecular weights were present. However, the most potent ACE inhibition was observed in WED I with an IC50 value of 116.90±2.25 μg/mL. Potent inhibitory effects on the production of inflammatory mediators were observed in the extracts of doenjang with molecular weights >1 kDa (WED II, WED III, and WED V), as demonstrated by the inhibition of lipopolysaccharide induced NO production in macrophages.

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Authors and Affiliations

  1. Department of Smart Foods and Drugs, Inje University, Gimhae, Gyeongnam, 621-749, Korea

    Sang Hyun Lee & Jae Han Jung

  2. Department of Food and Life Science, Inje University, Gimhae, Gyeongnam, 621-749, Korea

    Hyun A. Lee & Jae Cherl

  3. Ocular Neovascular Disease Research Center, Inje University Busan Paik Hospital, Busan, 614-735, Korea

    Hye-Sook Lee

  4. Department of Aquatic Life Medicine, Pukyong National University, Busan, 608-737, Korea

    Hyun Do Jeong

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  1. Sang Hyun Lee
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Correspondence to Jae Cherl.

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Lee, S.H., Jung, J.H., Lee, H.A. et al. Fractionation of nitrogenous compounds from water extracts of doenjang and their physiological activities. Food Sci Biotechnol 23, 523–529 (2014). https://doi.org/10.1007/s10068-014-0072-x

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  • DOI: https://doi.org/10.1007/s10068-014-0072-x

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