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Extensive host range determination and improved efficacy of the bacteriophage JBP901 in the presence of divalent cations for control of Bacillus cereus in Cheonggukjang

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Abstract

The Bacillus cereus specific bacteriophage JBP901 has a broad host range spectrum among B. cereus isolates. JBP901, which belongs to the SPO1 group in the family Myoviridae, lyses 279 of 344 (81%) food and human isolates. B. subtilis (50 food isolates) and B. licheniformis (12 food isolates) were both insenstive to JBP901. JBP901 completely eradicated B. cereus in the fast fermented soybean product cheonggukjang with addition of a divalent cation in a food application study. Divalent and monovalent cations play essential roles in adsorption and post-adsorption phases of JBP901 infection, respectively.

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Authors and Affiliations

  1. Department of Food Science and Technology, College of Agriculture and Life Sciences, Jeonbuk National University, Jeonju, Jeonbuk, 561-756, Korea

    Nadeeka Bandara & Kwang-Pyo Kim

  2. Jeollabukdo Institute of Health & Environment Research, Jeonbuk, 566-806, Korea

    Chyeon Hyeon Kim

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  1. Nadeeka Bandara
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  2. Chyeon Hyeon Kim
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  3. Kwang-Pyo Kim
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Correspondence to Kwang-Pyo Kim.

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Bandara, N., Kim, C.H. & Kim, KP. Extensive host range determination and improved efficacy of the bacteriophage JBP901 in the presence of divalent cations for control of Bacillus cereus in Cheonggukjang . Food Sci Biotechnol 23, 499–504 (2014). https://doi.org/10.1007/s10068-014-0068-6

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  • DOI: https://doi.org/10.1007/s10068-014-0068-6

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