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Effect of ionic strength on soluble protein removal from giant squid mantle (Dosidicus gigas) and functional evaluation of protein recovery

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An Erratum to this article was published on 21 May 2015

Abstract

Myofibrillar protein are the principally responsible of gelling properties in fishery resource, hence, during protein concentrate or isolated proteins preparation, sarcoplasmic protein are discarded; however, myofibrillar protein can support low levels of sarcoplasmic proteins without affecting the gelling property. Therefore, the aim of this study was to gradually remove sarcoplasmic proteins from giant squid mantle by means of different ionic strengths (I). Solutions of NaCl with different ionic strengths (I=0.0, 0.1, and 0.3) were used to obtain 3 protein concentrates. The electrophoretic profile in SDS-PAGE showed differences in protein removal with a high solubility of mantle proteins. The texture profile analysis showed that hardness increased in mantle protein washed with higher I. The total reactive sulfhydryls showed significant changes (p<0.05) detecting major formation of S-S bonds with protein removal at an I of 0.3. Differential scanning calorimetry showed a minor denaturation temperature of the actomyosin complex when protein removal was performed with an I of 0.3. The present study indicates that removal of sarcoplasmic protein as a function of I results in better quality gels.

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Authors and Affiliations

  1. Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Encinas y Rosales s/n. Hermosillo, Sonora, 83000, México

    Jose de Jesus Encinas-Arzate & Victor Manuel Ocaño-Higuera

  2. Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Encinas y Rosales s/n. Hermosillo, Sonora, 83000, México

    Josafat Marina Ezquerra-Brauer, Benjamin Ramirez-Wong, Wilfrido Torres-Arreaola & Enrique Marquez-Rios

  3. Departamento de Polímeros., Universidad de Sonora, Encinas y Rosales s/n. Hermosillo, Sonora, 83000, México

    Lorena Armenta-Villegas

Authors
  1. Jose de Jesus Encinas-Arzate
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  2. Josafat Marina Ezquerra-Brauer
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  3. Victor Manuel Ocaño-Higuera
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  4. Benjamin Ramirez-Wong
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  5. Lorena Armenta-Villegas
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  6. Wilfrido Torres-Arreaola
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  7. Enrique Marquez-Rios
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Correspondence to Enrique Marquez-Rios.

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Encinas-Arzate, J.d.J., Ezquerra-Brauer, J.M., Ocaño-Higuera, V.M. et al. Effect of ionic strength on soluble protein removal from giant squid mantle (Dosidicus gigas) and functional evaluation of protein recovery. Food Sci Biotechnol 23, 401–407 (2014). https://doi.org/10.1007/s10068-014-0055-y

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  • DOI: https://doi.org/10.1007/s10068-014-0055-y

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