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Production of palm oil-based diacylglycerol using Lecitase Ultra-catalyzed glycerolysis and molecular distillation

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Abstract

Diacylglycerol (DAG) was prepared via glycerolysis of palm oil catalyzed by Lecitase Ultra (LU), a novel phospholipase from the fusion of lipase genes from Thermomyces lanuginose and phospholipase genes from Fusarium oxysporum. Glycerolysis was performed in a solvent-free system. The optimized reaction conditions were: a glycerol/palm oil mole ratio of 7.5:1, initial substrate water content of 5%, substrate enzyme load of 2%, reaction temperature of 40°C, and reaction time of 8 h. In a scale-up reaction, a DAG content of 59.5% in the lipid layer was achieved. Through a two-step molecular distillation, the composition of the target product was 88.1% DAG, 2.8% TAG, 9.0% MAG, and 0.1% FFA. The fatty acid composition of the DAG oil, determined using GC-MS, was enriched compared with the original palm oil.

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Authors and Affiliations

  1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, 510640, China

    Ning Liu, Qiangzhong Zhao & Mouming Zhao

  2. Department of Food Science and Engineering, Jinan University, Guangzhou, 510632, China

    Yong Wang

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  1. Ning Liu
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  2. Yong Wang
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  3. Qiangzhong Zhao
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  4. Mouming Zhao
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Correspondence to Mouming Zhao.

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Liu, N., Wang, Y., Zhao, Q. et al. Production of palm oil-based diacylglycerol using Lecitase Ultra-catalyzed glycerolysis and molecular distillation. Food Sci Biotechnol 23, 365–371 (2014). https://doi.org/10.1007/s10068-014-0051-2

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  • DOI: https://doi.org/10.1007/s10068-014-0051-2

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