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Extracts of the seaweed Sargassum macrocarpum inhibit the CpG-induced inflammatory response by attenuating the NF-κB pathway

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Abstract

The anti-inflammatory effects of extracts of the seaweed Sargassum macrocarpum (SME) in bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs) were investigated. Primary BMDMs and BMDCs were used for cytokine production and western blot analysis. SME (0–50 μg/mL) pre-treatment resulted in a strong inhibitory effect against interleukin (IL)-12 p40, IL-6, and tumor necrosis factor (TNF)-α production in CpG-stimulated BMDMs and BMDCs. SME pre-treatment caused a strong inhibitory effect against NF-κB activation, as evaluated using degradation of IκBα and the NF-κB reporter gene assay. SME has significant anti-inflammatory properties and warrants further study regarding potential use as a medicinal food.

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References

  1. Kawai T, Akira S. The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nat. Immunol. 11: 373–384 (2010)

    Article  CAS  Google Scholar 

  2. Efron PA, Tsujimoto H, Bahjat FR, Ungaro R, Debernardis J, Tannahill C, Baker HV, Edwards CK, Moldawer LL. Differential maturation of murine bone-marrow derived dendritic cells with lipopolysaccharide and tumor necrosis factor-α. J. Endotoxin Res. 11: 145–160 (2005)

    CAS  Google Scholar 

  3. Yuk JM, Jo EK. Toll-like receptors and innate immunity. J. Bacteriol. Virol. 41: 225–235 (2011)

    Article  CAS  Google Scholar 

  4. Manzoor Z, Koh YS. Mitogen-activated protein kinases in inflammation. J. Bacteriol. Virol. 42: 189–195 (2012)

    Article  CAS  Google Scholar 

  5. Koh YS. Nucleic acid recognition and signaling by Toll-like receptor 9: compartment-dependent regulation. J. Bacteriol. Virol. 41: 131–132 (2011)

    Article  Google Scholar 

  6. Gilliet M, Cao W, Liu YJ. Plasmacytoid dendritic cells: Sensing nucleic acids in viral infection and autoimmune diseases. Nat. Rev. Immunol. 8: 594–606 (2008)

    Article  CAS  Google Scholar 

  7. Tsang CK, Ina A, Goto T, Kamei Y. Sargachromenol, a novel nerve growth factor-potentiating substance isolated from Sargassum macrocarpum, promotes neurite outgrowth and survival via distinct signaling pathways in PC12D cells. Neuroscience 132: 633–643 (2005)

    Article  CAS  Google Scholar 

  8. Kelman D, Posner EK, McDermid KJ, Tabandera NK, Wright PR, Wright AD. Antioxidant activity of Hawaiian marine algae. Mar. Drugs 10: 403–416 (2012)

    Article  CAS  Google Scholar 

  9. Yoon WJ, Heo SJ, Han SC, Lee HJ, Kang GJ, Kang HK, Hyun JW, Koh YS, Yoo ES. Anti-inflammatory effect of sargachromanol G isolated from Sargassum siliquastrum in RAW 264.7 cells. Arch. Pharm. Res. 35: 1421–1430 (2012)

    Article  CAS  Google Scholar 

  10. Lee OH, Yoon KY, Kim KJ, You SG, Lee BY. Seaweed extracts as a potential tool for the attenuation of oxidative damage in obesity-related pathologies. J. Phycol. 47: 548–556 (2011)

    Article  CAS  Google Scholar 

  11. Tsang CK, Kamei Y. Sargaquinoic acid supports the survival of neuronal PC12D cells in a nerve growth factor-independent manner. Eur. J. Pharmacol. 488: 11–18 (2004)

    Article  CAS  Google Scholar 

  12. Kamei Y, Sueyoshi M, Hayashi KI, Terada R, Hiroshi N. The novel anti-Propionibacterium acnes compound, Sargafuran, found in the marine brown alga Sargassum macrocarpum. J. Antibiot. 62: 259–263 (2009)

    Article  CAS  Google Scholar 

  13. Koo JE, Hong HJ, Dearth A, Kobayashi KS, Koh YS. Intracellular invasion of Orientia tsutsugamushi activates inflammasome in ASC-dependent manner. PLoS One 7: e39042 (2012)

    Article  CAS  Google Scholar 

  14. Koo JE, Hong HJ, Mathema VB, Kang HK, Hyun JW, Kim GY, Kim YR, Maeng YH, Hyun CL, Chang WY, Koh YS. Inhibitory effects of Carpinus tschonoskii leaves extract on CpG-stimulated pro-inflammatory cytokine production in murine bone marrow-derived macrophages and dendritic cells. In Vitro Cell. Dev. Biol. Anim. 4: 197–202 (2012)

    Article  Google Scholar 

  15. Feldmann M, Maini RN. Anti-TNF α therapy of rheumatoid arthritis: What have we learned? Annu. Rev. Immunol. 19: 163–196 (2001)

    Article  CAS  Google Scholar 

  16. Bromberg J, Wang TC, Inflammation and cancer: IL-6 and STAT3 complete the link. Cancer Cell 15: 79–80 (2009)

    Article  CAS  Google Scholar 

  17. Lee JC, Laydon JT, McDonnell PC, Gallagher TF, Kumar S, Green D, McNulty D, Blumenthal MJ, Keys JR, Landvatter SW, Strickler JE, McLaughlin MM, Siemens IR, Fisher SM, Livi GP, White JR, Adams JL, Young PR. A protein kinase involved in the regulation of inflammatory cytokine biosynthesis. Nature 372: 739–746 (1994)

    Article  CAS  Google Scholar 

  18. Bao L, Lindgren JU, van der Meide P, Zhu S, Ljunggren HG, Zhu J. The critical role of IL-12 p40 in initiating, enhancing, and perpetuating pathogenic events in murine experimental autoimmune neuritis. Brain Pathol. 12: 420–429 (2002)

    Article  CAS  Google Scholar 

  19. Ishii KJ, Koyama S, Nakagawa A, Coban C, Akira S. Host innate immune receptors and beyond: Making sense of microbial infections. Cell Host Microbe 3: 352–363 (2008)

    Article  CAS  Google Scholar 

  20. Trikha M, Corringham R, Klein B, Rossi JF. Targeted antiinterleukin-6 monoclonal antibody therapy for cancer: A review of the rationale and clinical evidence. Clin. Cancer Res. 9: 4653–4665 (2003)

    CAS  Google Scholar 

  21. McInnes IB, Leung BP, Sturrock RD, Field M, Liew FY. Interleukin-15 mediates T cell-dependent regulation of tumor necrosis factor-alpha production in rheumatoid arthritis. Nat. Med. 3: 189–195 (1997)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. School of Medicine, Jeju National University, Jeju, 690-756, Korea

    Zahid Manzoor, Vivek Bhakta Mathema, Doobyeong Chae, Eun-Sook Yoo, Hee-Kyoung Kang, Jin-Won Hyun & Young-Sang Koh

  2. Institute of Medical Science, Jeju National University, Jeju, 690-756, Korea

    Zahid Manzoor, Vivek Bhakta Mathema, Doobyeong Chae, Eun-Sook Yoo, Hee-Kyoung Kang, Jin-Won Hyun & Young-Sang Koh

  3. Department of Chemistry, College of Natural Sciences, Jeju National University, Jeju, 690-756, Korea

    Nam Ho Lee

  4. Jeju Biodiversity Research Institute, Jeju Technopark, Jeju, 699-943, Korea

    Mi-Hee Ko

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  1. Zahid Manzoor
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  2. Vivek Bhakta Mathema
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  9. Young-Sang Koh
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Correspondence to Young-Sang Koh.

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Manzoor, Z., Mathema, V.B., Chae, D. et al. Extracts of the seaweed Sargassum macrocarpum inhibit the CpG-induced inflammatory response by attenuating the NF-κB pathway. Food Sci Biotechnol 23, 293–297 (2014). https://doi.org/10.1007/s10068-014-0041-4

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  • DOI: https://doi.org/10.1007/s10068-014-0041-4

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