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Antimutagenicity activity of the putative probiotic strain Lactobacillus paracasei subsp. tolerans JG22 isolated from pepper leaves Jangajji

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Abstract

The aim of this study was to evaluate the probiotic nature, antimutagenicity, and mutagen-binding ability of the bacterial strain JG22 isolated from pepper-leaf jangajji. The strain, which was identified as Lactobacillus paracasei subsp. tolerans JG22, retained a high survival rate when exposed to pepsin-containing gastric juice and intestinal fluid supplemented with oxgall. This strain adhered to Caco-2 cells and exhibited resistance to ciprofloxacin, rifampicin, erythromycin, and cephalothin. Furthermore, against the 3 mutagens tested, live cells of strain JG22 displayed the greatest antimutagenicity against 2-nitroflourene (2-NF), with 51.37% inhibition, but exhibited relatively low antimutagenicity (11.07%) against 4-nitroquinoline-1-oxide (4-NQO). The antimutagenic effect of this strain was somewhat reduced after heat treatment for 30 min at 80°C. The most efficient mutagen-binding was observed with live cells of strain JG22, which bound 69.5% of the 2-NF. Thus, the putative probiotic strain JG22 could play a vital role in reducing the risk of cancer by absorbing mutagens and suppressing mutagenesis. Therefore, we consider the strain a good candidate for functional cultures and food system development.

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  1. Department of Food Nutrition and Science, Tongmyong University, Busan, 608-711, Korea

    Sung-Mee Lim

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Lim, SM. Antimutagenicity activity of the putative probiotic strain Lactobacillus paracasei subsp. tolerans JG22 isolated from pepper leaves Jangajji. Food Sci Biotechnol 23, 141–150 (2014). https://doi.org/10.1007/s10068-014-0019-2

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