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Effect of matrix metalloproteinase on autolysis of sea cucumber Stichopus japonicus

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Abstract

To investigate the relationship between matrix metalloproteinase (MMPs) and autolysis of sea cucumber Stichopus japonicus, the dermis homogenate was incubated at 25°C to induce autolysis. EDTA Na2 and 1,10-phenanthroline were used to verify the effect of MMPs on autolysis, which was measured by soluble protein and protein pattern. Soluble protein level increased during a 6-h autolysis process. SDS-PAGE demonstrated obvious protein degradation with the concomitant occurrence of degradation products. The above two indicators could be inhibited significantly by EDTA Na2 and 1,10-phenanthroline, indicating that MMPs might play a significant role in autolysis of sea cucumber.

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Authors and Affiliations

  1. School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116034, China

    Li-Ming Sun, Ting-Ting Wang, Bei-Wei Zhu, Hai-Ling Niu, Rui Zhang, Hong-Man Hou & Gong-Liang Zhang

  2. Department of Biological Resources Chemistry, Faculty of Agriculture, Okayama University, Okayama, 700-8530, Japan

    Yoshiyuki Murata

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  1. Li-Ming Sun
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  2. Ting-Ting Wang
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  4. Hai-Ling Niu
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  5. Rui Zhang
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  6. Hong-Man Hou
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  7. Gong-Liang Zhang
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  8. Yoshiyuki Murata
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Correspondence to Bei-Wei Zhu.

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Sun, LM., Wang, TT., Zhu, BW. et al. Effect of matrix metalloproteinase on autolysis of sea cucumber Stichopus japonicus . Food Sci Biotechnol 22, 1–3 (2013). https://doi.org/10.1007/s10068-013-0210-x

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  • DOI: https://doi.org/10.1007/s10068-013-0210-x

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