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Effect of UV on stability of collagen with consideration of hydratation and fibrillogenesis

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Abstract

Collagen is an abundant extracellular matrix protein that is widely used in the biomaterial, food, and medical fields. Most extracted collagen is soluble in acids and assembles into fibrils under neutral pH conditions. In order to study the effect of UV irradiation on the stability of collagen with consideration of hydratation and fibrillogenesis, UV irradiation of collagen under acid and neutral pH conditions was investigated using electrophoresis, turbidity analysis, and Fourier transform infrared spectroscopy. The relationship between the collagen structure and sensitivity to UV radiation was assessed by studying the effect of UV on collagen under different pH conditions. Results showed that structural changes in collagen caused by UV depend on the degree of hydratation. Soluble collagen completely loses fibrillogeneic ability after exposure to UV for 40 min. Fibrillogenesis of collagen can relieve the UV effect to some degree.

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Authors and Affiliations

  1. School of Environmental Science and Engineering, Chang’an University, Xi’an, Shaanxi, 710054, China

    Jian-Yu Xing, Bo Bai & Ming-Yan Yang

  2. Shaanxi Provincial Institute of Microbiology, Xi’an, Shaanxi, 710043, China

    Wen-Jiao Xue

  3. Opening Research Laboratory of Water Resources and Land Environment in Arid and Semiarid Regions of Ministry of Land Resources, Chang’an University, Xi’an, Shaanxi, 710054, China

    Jian-Yu Xing

Authors
  1. Jian-Yu Xing
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  2. Bo Bai
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  3. Wen-Jiao Xue
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  4. Ming-Yan Yang
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Correspondence to Jian-Yu Xing.

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Xing, JY., Bai, B., Xue, WJ. et al. Effect of UV on stability of collagen with consideration of hydratation and fibrillogenesis. Food Sci Biotechnol 22, 1–5 (2013). https://doi.org/10.1007/s10068-013-0204-8

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  • DOI: https://doi.org/10.1007/s10068-013-0204-8

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